Waterborne outbreak of Escherichia coli 0157

Dev, Vikram J.; Main, Michael L.; Gould, I. M.

doi:10.1016/0140-6736(91)93092-n

Cited by 110 publications

(55 citation statements)

References 2 publications

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“…Although STEC prevalence in water samples was low, the importance of water as a vehicle of disease transmission cannot be disregarded. Many outbreaks of gastrointestinal diseases have been reported and evidences indicate the importance of water in STEC epidemiology (14,17,25,30,45). Moreover, the etiological agent is not recovered in most gastrointestinal disease outbreaks involving water (41).…”

Section: Resultsmentioning

confidence: 99%

Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms

Vicente¹,

Amaral²,

Cerqueira³

2005

Braz. J. Microbiol.

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The objective of this study was to determine the prevalence of Shigatoxigenic Escherichia coli (STEC) and STEC serogroups O157, O111 and O113 in feces, water and milk sampled in dairy farms in Jaboticabal, SP, Brazil. Feces (n=454), water (n=54) and milk samples (n=30) were collected from 10 herds and assessed for the presence of the virulence genes stx 1 , stx 2 and eae by polymerase chain reaction (PCR). All stx and eae positive samples were submitted to a second PCR reaction targeting the sequences rfb O157, rfb O111 and rfb O113. High prevalence of stx was detected (59.9%) in fecal samples, whereas the prevalence of sequences rfb O157, rfb O111 and rfb O113 was 18.9%, 3.3% and 30.4%, respectively. All sequences were detected more frequently in calves and heifers. Sequences stx2 and eae were prevalent in the fecal samples. stx sequences were detected in 1.9% and 3.3% of water and milk samples, respectively. Low prevalence of E. coli O113 was observed in water samples, whereas no E. coli O157 or E. coli O111 was detected. Furthermore, none of the serogroups were identified in milk samples. STEC was identified in all herds (100%), and serogroups O157, O111 and O113 were observed in 40%, 50% and 90% of the herds, respectively. In conclusion, the high STEC prevalence detected in dairy herds evidences that bovine feces might play an important role as a contamination source in the region of Jaboticabal, Brazil.

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Section: Resultsmentioning

confidence: 99%

Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms

Vicente¹,

Amaral²,

Cerqueira³

2005

Braz. J. Microbiol.

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“…and Campylobacter spp. amongst others [40][41][42]. Studies carried out in different regions of Asia, Africa and South America have frequently pointed the unavailability of potable water for various activities at the vending site as a major concern.…”

Section: Watermentioning

confidence: 99%

Street Vended Food in Developing World: Hazard Analyses

Rane¹

2011

Indian J Microbiol

259

203

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Street food vending has become an important public health issue and a great concern to everybody. This is due to widespread food borne diseases, due to the mushrooming of wayside food vendors who lack an adequate understanding of the basic food safety issues. Major sources contributing to microbial contamination are the place of preparation, utensils for cooking and serving, raw materials, time and temperature abuse of cooked foods and the personal hygiene of vendors. Various studies have identified the sources of food safety issues involved in street foods to be microorganism belonging to the genus Bacillus, Staphylococcus, Clostridium, Vibrio, Campylobacter, Listeria, Salmonella. Application of sound risk analysis policies is being advocated to provide a scientific base to the host of risk management option which India may need to explore to ensure public health and safety.

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“…These infections occur in all parts of the world including Middle East countries (Lerman et al, 1992;Adwan et al, 2002). Identified human infections were traced to eating undercooked beef (Chapman et al, 1993;Morgan et al, 1993;Bell et al, 1994), drinking contaminated water (Dev et al, 1991;Swerdlow et al, 1992;Danon-Schaffer, 2001), and, sporadically, to a direct contact with infected animals (Rice et al, 1996;Trevena et al, 1996;Chalmers et al, 1997;Chapman et al, 2000).…”

mentioning

confidence: 99%

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7

Novotná¹,

Alexa²,

Hamrik³

et al. 2005

Vet. Med. - Czech

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Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were taken and examined for Shiga toxin-producing Escherichia coli (STEC). The bacteriological examination included the preenrichment of rectal swabs in EC broth with novobiocin, and a subsequent parallel isolation on enterohemolysin agar and immunomagnetic separation with cultivation on CT-SMAC. The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1-to 3-weeks old in one sheep herd with intensive milk production. In the remaining three herds, serogroups O128, O78, O15 and serotype O128:K85 of STEC strains were the most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed by PCR. In two untypable STEC isolates, stx2 and ehlyA genes were detected. In other STEC non-O157 isolates, only stx1 a ehlyA genes were found. All STEC O157:H7 isolates were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicillin and streptomycin, one against co-trimoxazole. One isolate was resistant against ampicillin, ampicillin-sulbactam, cephalosporins (cefazolin, cefuroxime), monobactams (aztreonam), sulphonamides, co-trimoxazole, aminoglycosides, tetracycline and chloramphenicol. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD pattern. Of 36 STEC non-O157 isolates, one isolate was resistant against sulphonamides and co-trimoxazole, and another one against ampicillin, streptomycin, sulphonamides and co-trimoxazole. STEC isolates resistant against antimicrobial agents were demonstrated only in herds with intensive management.

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Waterborne outbreak of Escherichia coli 0157

Cited by 110 publications

References 2 publications

Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms

Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms

Street Vended Food in Developing World: Hazard Analyses

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7

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