Wavefront coding using a spatial light modulator for extended depth of field microscopy

Gierlak, Michael; Albrecht, Stefanie; Kauer, Josef; Leverenz, Eric; Darvin, Maxim E.

doi:10.1117/12.2032618

Cited by 4 publications

(2 citation statements)

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“…After deriving the PSFs/OTFs, it is necessary to characterize their consistency to aberrations using a variety of metrics. The invariance of the PSF to aberrations can be characterized by Strehl ratio 89 , Fisher information 89,97 , mean square error 26,90,96,102 , and variance 103 . The consistency of MTF/OTF to aberrations can be characterized by Fisher information 64,72,73,85 , mean square error 74,80 , Hilbert space angle 68,69,72,76 , and standard deviation 104 .…”

Section: Phase Mask and Reconstruction Algorithmmentioning

confidence: 99%

Point spread function engineering based on wavefront phase modulation

Zhang,

Cui,

Yue

et al. 2023

Optoelectronic Imaging and Multimedia Technology X

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Section: Phase Mask and Reconstruction Algorithmmentioning

confidence: 99%

Point spread function engineering based on wavefront phase modulation

Zhang,

Cui,

Yue

et al. 2023

Optoelectronic Imaging and Multimedia Technology X

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“…Through use of the LC-SLM, different PSF parameters can be easily varied to extend the DOF by varying amounts. 28 The applicability of these results to live-cell microscopy was observed by imaging the distribution of MitoTracker-labeled mitochondria in a live epithelial lung-cell culture and in a relatively thick sample study by imaging the fine structure of fluorescently labeled brine shrimp anatomy. Previous EDF studies have shown 3-D structures that are relatively thin or sparsely distributed in the volume.…”

Section: Introductionmentioning

confidence: 97%

Performance evaluation of extended depth of field microscopy in the presence of spherical aberration and noise

2018

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Effectiveness of extended depth of field microscopy (EDFM) implementation with wavefront encoding methods is reduced by depth-induced spherical aberration (SA) due to reliance of this approach on a defined point spread function (PSF). Evaluation of the engineered PSF's robustness to SA, when a specific phase mask design is used, is presented in terms of the final restored image quality. Synthetic intermediate images were generated using selected generalized cubic and cubic phase mask designs. Experimental intermediate images were acquired using the same phase mask designs projected from a liquid crystal spatial light modulator. Intermediate images were restored using the penalized space-invariant expectation maximization and the regularized linear least squares algorithms. In the presence of depth-induced SA, systems characterized by radially symmetric PSFs, coupled with model-based computational methods, achieve microscope imaging performance with fewer deviations in structural fidelity (e.g., artifacts) in simulation and experiment and 50% more accurate positioning of 1-μm beads at 10-μm depth in simulation than those with radially asymmetric PSFs. Despite a drop in the signal-to-noise ratio after processing, EDFM is shown to achieve the conventional resolution limit when a model-based reconstruction algorithm with appropriate regularization is used. These trends are also found in images of fixed fluorescently labeled brine shrimp, not adjacent to the coverslip, and fluorescently labeled mitochondria in live cells.

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DL-EDOF: Novel Multi-Focus Image Data Set and Deep Learning-Based Approach for More Accurate and Specimen-Free Extended Depth of Focus

Dogan,

et al. 2024

J Digit Imaging. Inform. med.

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Wavefront coding using a spatial light modulator for extended depth of field microscopy

Cited by 4 publications

References 0 publications

Point spread function engineering based on wavefront phase modulation

Point spread function engineering based on wavefront phase modulation

Performance evaluation of extended depth of field microscopy in the presence of spherical aberration and noise

DL-EDOF: Novel Multi-Focus Image Data Set and Deep Learning-Based Approach for More Accurate and Specimen-Free Extended Depth of Focus

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