Wavefront sensorless adaptive optics optical coherence tomography for in vivo retinal imaging in mice

Jian, Yifan; Xu, Jing; Gradowski, Martin A.; Bonora, Stefano; Zawadzki, Robert J.; Sarunic, Marinko V.

doi:10.1364/boe.5.000547

Cited by 93 publications

(85 citation statements)

References 37 publications

(37 reference statements)

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“…As previously presented, a dynamic depth selection option and system controls for the DM were added to the software for enabling WSAO [36]. The GPU used during imaging was the Quadro K6000 (NVIDIA, Santa Clara, California) and was capable of achieving a 1024-point-A-scan processing rate of 1.9 MHz [43].…”

Section: Worktation and Software Specificationsmentioning

confidence: 99%

“…This resulted in an acquisition rate of 800 frames per second, equivalent to 10 volumes per second with processing and display in realtime. The WSAO optimization algorithm for human imaging was modified from our previous report on imaging mice [36]. For a pupil size of ~5 mm, correction of the fifth Zernike radial order has relatively low impact on the total RMS of the ocular aberrations for human imaging [44][45][46].…”

Section: Image Acquisition and Optimizationmentioning

confidence: 99%

“…In our previous report, we demonstrated a WSAO-OCT system for small animal imaging that was presented and evaluated on pigmented and albino mice [36]. We developed the WSAO-OCT technique using a modal control of the DM [37], which enabled a faster convergence rate in comparison to a stochastic approach.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography

Wong¹,

Jian²,

Cua³

et al. 2015

Biomed. Opt. Express

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Wavefront sensorless adaptive optics optical coherence tomography (WSAO-OCT) is a novel imaging technique for in vivo highresolution depth-resolved imaging that mitigates some of the challenges encountered with the use of sensor-based adaptive optics designs. This technique replaces the Hartmann Shack wavefront sensor used to measure aberrations with a depth-resolved image-driven optimization algorithm, with the metric based on the OCT volumes acquired in real-time. The custom-built ultrahigh-speed GPU processing platform and fast modal optimization algorithm presented in this paper was essential in enabling real-time, in vivo imaging of human retinas with wavefront sensorless AO correction. WSAO-OCT is especially advantageous for developing a clinical high-resolution retinal imaging system as it enables the use of a compact, low-cost and robust lens-based adaptive optics design. In this report, we describe our WSAO-OCT system for imaging the human photoreceptor mosaic in vivo. We validated our system performance by imaging the retina at several eccentricities, and demonstrated the improvement in photoreceptor visibility with WSAO compensation.

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Section: Worktation and Software Specificationsmentioning

confidence: 99%

Section: Image Acquisition and Optimizationmentioning

confidence: 99%

See 1 more Smart Citation

In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography

Wong¹,

Jian²,

Cua³

et al. 2015

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, reports of in vivo imaging of the mouse retina with AO-enhanced SLO and OCT have only recently been published [2][3][4][5][6][7][8][9]. AO imaging of the mouse retina has been delayed by the challenge of designing a system for an eye ten-fold smaller than that of the human, and by the availability of highly developed ex vivo histochemical retinal imaging methods.…”

Section: Introductionmentioning

confidence: 99%

Adaptive-optics SLO imaging combined with widefield OCT and SLO enables precise 3D localization of fluorescent cells in the mouse retina

Zawadzki

Zhang

Zam

et al. 2015

Biomed. Opt. Express

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Adaptive optics scanning laser ophthalmoscopy (AO-SLO) has recently been used to achieve exquisite subcellular resolution imaging of the mouse retina. Wavefront sensing-based AO typically restricts the field of view to a few degrees of visual angle. As a consequence the relationship between AO-SLO data and larger scale retinal structures and cellular patterns can be difficult to assess. The retinal vasculature affords a largescale 3D map on which cells and structures can be located during in vivo imaging. Phase-variance OCT (pv-OCT) can efficiently image the vasculature with near-infrared light in a label-free manner, allowing 3D vascular reconstruction with high precision. We combined widefield pv-OCT and SLO imaging with AO-SLO reflection and fluorescence imaging to localize two types of fluorescent cells within the retinal layers: GFPexpressing microglia, the resident macrophages of the retina, and GFPexpressing cone photoreceptor cells. We describe in detail a reflective afocal AO-SLO retinal imaging system designed for high resolution retinal imaging in mice. The optical performance of this instrument is compared to other state-of-the-art AO-based mouse retinal imaging systems. The spatial and temporal resolution of the new AO instrumentation was characterized with angiography of retinal capillaries, including blood-flow velocity analysis. Depth-resolved AO-SLO fluorescent images of microglia and cone photoreceptors are visualized in parallel with 469 nm and 663 nm reflectance images of the microvasculature and other structures. Additional applications of the new instrumentation are discussed.

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“…A notable development trend in current AO retinal imaging is the sensorless correction that based upon optimization of the retinal images brightness [51][52][53][54][55]. This mechanism is dictated by the imaging frame rate and requires long time (~10 seconds) to correct the wave aberration.…”

Section: Discussionmentioning

confidence: 99%

High-speed adaptive optics for imaging of the living human eye

Zhang

Meadway

et al. 2015

Opt. Express

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Abstract:The discovery of high frequency temporal fluctuation of human ocular wave aberration dictates the necessity of high speed adaptive optics (AO) correction for high resolution retinal imaging. We present a high speed AO system for an experimental adaptive optics scanning laser ophthalmoscope (AOSLO). We developed a custom high speed ShackHartmann wavefront sensor and maximized the wavefront detection speed based upon a trade-off among the wavefront spatial sampling density, the dynamic range, and the measurement sensitivity. We examined the temporal dynamic property of the ocular wavefront under the AOSLO imaging condition and improved the dual-thread AO control strategy. The high speed AO can be operated with a closed-loop frequency up to 110 Hz. Experiment results demonstrated that the high speed AO system can provide improved compensation for the wave aberration up to 30 Hz in the living human eye.

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Wavefront sensorless adaptive optics optical coherence tomography for in vivo retinal imaging in mice

Cited by 93 publications

References 37 publications

In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography

In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography

Adaptive-optics SLO imaging combined with widefield OCT and SLO enables precise 3D localization of fluorescent cells in the mouse retina

High-speed adaptive optics for imaging of the living human eye

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