The study of human cardiovascular activity is one of the main methods for assessing the health of the human. It is performed in clinical conditions via electrocardiographic devices and in the daily life of a individuals through Holter monitoring. An important diagnostic parameter that can be determined by an electrocardiogram, taking into account the difference between successive heartbeat is heart rate variability – a widely used non-invasive method of measuring heart rate. This parameter makes it possible to assess the risk of various cardiac diseases such as angina, cardiac infarction, life-threatening arrhythmias, etc. This chapter presents the morphological bases of the cardio records, heart rate variability, and its impact on the healthy status of the individual. It describes the created cardiology base of prolongated Holter recordings for the purposes of scientific research project. Presented are internationally approved standards to provide web accessibility to internet-based data bases and other resources for people with disabilities.