Weak Chemiluminescence of Oxidation Reactions

Stauff, J.; Schmidkunz, H.; Hartmann, G.

doi:10.1038/198281a0

Cited by 78 publications

(17 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Allen, Stjernholm, and Steele have suggested that singlet oxygen (102) could be that agent (28). This electronically excited form of oxygen appears to participate in ordinary chemical reactions involving molecular oxygen (36,37) where its presence has been monitored by its generation of luminescence (36)(37)(38)(39)(40)(41)(42). To our knowledge no direct proof that this species is generated by cells has been presented.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.

Johnston¹,

Keele²,

Misra³

et al. 1975

J. Clin. Invest.

652

194

View full text Add to dashboard Cite

A B S T R A C T The capacity of human phagocytes to generate superoxide anion (02-), a free radical of oxygen, and a possible role for this radical or its derivatives in the killing of phagocytized bacteria were explored using leukocytes from normal individuals and patients with chronic granulomatous disease (CGD). Superoxide dismutase, which removes 02-, consistently inhibited phagocytosis-associated nitroblue tetrazolium (NBT) reduction indicating the involvement of O2 in this process. Similarly, superoxide dismutase inhibited the luminescence that occurs with phagocytosis, implicating 0-in this phenomenon, perhaps through its spontaneous dismutation into singlet oxygen. Subcellular fractions from homogenates of both normal and CGD leukocytes generated 02-effectively in the presence of NADH as substrate. However strating the interrelationship between NBT reduction and 02 generation in phagocytizing cells. Activity of superoxide dismutase, the enzyme responsible for protecting the cell from the damaging effects of 02-, was approximately equal in homogenates of normal and CGD granulocytes. Polyacrylamide electrophoresis separated this activity into a minor band that appeared to be the manganese-containing superoxide dismutase associated with mitochondria and a more concentrated, cyanide-sensitive, cytosol form of the enzyme with electrophoretic mobility that corresponded to that of erythrocyte cuprozinc superoxide dismutase.Superoxide dismutase inhibited the phagocytic killing of Escherichia coli, Staphylococcus aureus, and Streptococcus viridans. A similar inhibitory effect was noted with catalase which removes hydrogen peroxide. Neither enzyme inhibited the ingestion of bacteria. Peroxide and 02-are believed to interact to generate the potent oxidant, hydroxyl radical (-OH). A requirement for -OH in the phagocytic bactericidal event might explain the apparent requirement for both 02-and H202 for such activity. In agreement with this possibility, benzoate and mannitol, scavengers of -OH, inhibited phagocytic bactericidal activity. Generation of singlet oxygen from 02-and -OH also might explain these findings.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Several laboratories have reported that the aerobic xanthine-xanthine oxidase reaction, which generates Of, can generate chemiluminescence (38)(39)(40)(41)(42) (42). However, the enzyme-catalyzed dismutation does not give rise to '02 (41,42), which could explain the inhibition of chemiluminescence by SOD.…”

Section: Discussionmentioning

confidence: 99%

The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.

Johnston¹,

Keele²,

Misra³

et al. 1975

J. Clin. Invest.

652

194

View full text Add to dashboard Cite

show abstract

“…The molecular mechanism for the generation of excited species are apparently different from those described above, and seem to be multifaceted. This type of superoxide dismutase-sensitive photoemission can be partly explained as originating from the spontaneous disproportionation of 02-' (reaction 9), known to produce 102' (9) Although theoretical and experimental evidence is available to support this reaction (Stauff et al, 1963;Khan, 1981;Koppenol, 1976), evidence against this has been brought forward (Foote et al, 1981), and its likelihood in biological systems remains under debate.…”

Section: Chemiluminescence During Redox Transitions Of Quinonesmentioning

confidence: 96%

Biological Chemiluminescence

Cadenas¹

1988

Reactive Oxygen Species in Chemistry, Biology, and Medicine

View full text Add to dashboard Cite

“…2 owing to the formation of singlet oxygen molecular pair, ( 1 O 2 ) 2 * , which has higher energy than the ground state triplet oxygen. CL phenomena occur when the molecular pair lose excess energy and turn to the latter according to reactions (6)- (9) [19,20,23,24]. …”

Section: The Chemiluminescence Mechanism Of Pga In the Pms/co(ii) Systemmentioning

confidence: 99%

Determination of folic acid by chemiluminescence based on peroxomonosulfate-cobalt(II) system

Zhang

Zhao

Lin

2008

Talanta

119

View full text Add to dashboard Cite

Based on the chemiluminescence (CL) phenomena of folic acid in peroxomonosulfate-cobalt(II) system, a rapid and sensitive CL method was developed for determination of folic acid in pharmaceutical preparations and its urinary metabolism processes. Under the optimum conditions, the relative CL intensity was linear over the concentration ranging from 10 −9 to 8 × 10 −7 mol L −1 (R 2 = 0.9991) with a detection limit as low as 6 × 10 −10 mol L −1 (S/N = 3) and relative standard deviation was 2.63% for 2 × 10 −8 mol L −1 folic acid (n = 11). This method has been successfully applied to the determination of folic acid in tablets and human urine. The blank CL emission was yielded owing to the formation of singlet oxygen molecular pair from the quenching experiment of 1,4-diazabicyclo[2.2.2]octane, and pterine-6-carboxylic acid might be the degradation intermediate in this system and it also acts an energy acceptor and sensitizes the chemiluminescence based on the studies of the CL and fluorescence spectra.

show abstract

Weak Chemiluminescence of Oxidation Reactions

Cited by 78 publications

References 7 publications

The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.

The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.

Biological Chemiluminescence

Determination of folic acid by chemiluminescence based on peroxomonosulfate-cobalt(II) system

Contact Info

Product

Resources

About