Since the 21st century, attempts have been made to reform English teaching in colleges, but due to the influence of concepts such as exam oriented, utilitarian, and instrumental, English teaching in colleges still focuses on injection based teaching. Teaching does not place much emphasis on the use and improvement of sophisticated language abilities, which is not helpful to developing global capabilities. Therefore, the university English teaching mode needs innovation. This article would explore its innovation from a cultural perspective, first explaining the necessity of innovation in the university English teaching mode, as well as the existing problems, and then constructing a new university English teaching mode based on a cultural perspective. Finally, taking R University as an example, this article selects students from Class 1 and Class 2 of the 2018 English major at the university as the experimental subjects, with Class 1 as the experimental group, and applies a cultural perspective based college English teaching model. Class 2 as the control group, and for the comparative experimental analysis, it is taught using conventional methods. After comparing the experiments, it was found that the percentage of people who did well in Class 1 on the post-experimental mapping test increased by 40% compared to the pre-experimental period, while the proportion of people with excellent grades in the control group increased by 5%. In terms of the assessment of English comprehensive application ability before and after the experiment, the number of people in the experimental group with good and excellent English comprehensive application ability increased the most, while the control group showed changes, but the changes were not significant, and the improvement of learning ability was not fast. In summary, the college English teaching model based on cultural perspective is significantly superior to traditional teaching models, with high feasibility, and is conducive to improving students' cross-cultural communication ability and cultural quality.