An experiment was conducted to assess the impact of flucetosulfuron, a new generation sulfonylurea herbicide, on weed seed bank dynamics of wet land paddy field. Field experiments were carried out during two consecutive seasons (Kharif 2016 and Rabi 2016-'17), to assess the bio-efficacy of flucetosulfuron in wet seeded rice which was accompanied by weed seed bank assay. Weed seed bank assay of the soil was carried out before and after the field experiments in both the seasons by the seedling emergence method. The experiment on weed seed bank was laid out in Completely Randomised Block Design (CRD) with 12 treatments replicated thrice. Flucetosulfuron @ 20, 25, and 30 g ha -1 applied at 2-3, 10-12, and 18-20 days after sowing (DAS) along with two control treatments viz., hand weeding at 20 and 40 DAS and unweeded control comprised the treatments. The emerging weeds were uprooted at 14 days interval up to a period of two months; categorized as grasses, broad leaved weeds, and sedges and counted. Results revealed that non-herbicidal plots recorded significantly higher count of total emerged weeds compared to herbicide applied plots. In all the herbicide applied plots, effective reduction in weed seed bank could be obtained irrespective of the dose of the herbicide. Regarding the time of application, flucetosulfuron applied at 10-12 and 18-20 DAS recorded significantly lower weed seed bank during both the seasons, compared to its application at 2-3 DAS.