Weighted Gene Co-expression Network Analysis Revealed That CircMARK3 Is a Potential CircRNA Affects Fat Deposition in Buffalo

Xue, Feng; Jian-Gao, Zhao; Li, Fen; Aloufi, Bandar Hamad; Alshammari, Ahmed Mohajja; Ma, Yun

doi:10.3389/fvets.2022.946447

Cited by 6 publications

(6 citation statements)

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“…The binding of plasma triglyceride fatty acids to white adipose tissue is LPL-dependent and the enzyme is regulated by angiopoietin-like protein-4 (ANGPTL-4), an unfolding molecular chaperone that converts active LPL dimers to inactive monomers [ 41 ]. Feng et al [ 24 ] reported that circMARK3 promoted the adipogenic differentiation of buffalo adipocytes and 3T3-L1 cells by up-regulating the expression levels of lipogenic marker genes PPARγ , C/EBPα , and FABP4 . FABP4 is involved in fatty acid transport and plays an important role in animal fat deposition [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…CircRNA binds miRNAs through a sponge, preventing miRNAs from bindings to their target genes to regulate mRNA expression by blocking their binding [ 21 ]. For instance, circSAMD4A promotes the differentiation of preadipocytes by acting as an miR-138-5p sponge in humans [ 22 ], while the sus_circPAPPA2 inhibited the fat deposition of ovariectomized pigs through the miR-2366/glycoprotein k (GK) pathway [ 23 , 24 ]. The circRNA/miRNA interaction reveals a new epigenetic regulatory layer.…”

Section: Introductionmentioning

confidence: 99%

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CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

Xue

et al. 2023

Cells

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Circular RNAs (CircRNAs) are covalently closed-loop non-coding RNA (ncRNA) molecules present in eukaryotes. Numerous studies have demonstrated that circRNAs are important regulators of bovine fat deposition, but their precise mechanisms remain unclear. Previous transcriptome sequencing studies have indicated that circADAMTS16, a circRNA derived from the a disintegrin-like metalloproteinases with the thrombospondin motif 16 (ADAMTS16) gene, is high expressed in bovine adipose tissue. This gives a hint that the circRNA may be involved in the process of bovine lipid metabolism. In this study, the targeting relationship between circADAMTS16 and miR-10167-3p was verified using a dual-luciferase reporter assay. Then, the functions of circADAMTS16 and miR-10167-3p in bovine adipocytes were explored through gain-of-function and lose-of-function. The mRNA expression levels of genes were detected by real-time quantitative PCR (qPCR), and lipid droplet formation was phenotypically evaluated by Oil Red O staining. Cell proliferation and apoptosis were detected using CCK-8, EdU, and flow cytometry. We demonstrated that circADAMTS16 targeted binding to miR-10167-3p. The up-regulation of circADAMTS16 inhibited the differentiation of bovine preadipocytes, and the overexpression of miR-10167-3p promoted the differentiation of bovine preadipocytes. Meanwhile, CCK-8 and EdU results indicated that circADAMTS16 promoted adipocyte proliferation. Subsequently, flow cytometry analysis showed that circADAMTS16 promoted cell transition from G0/G1 phase to S phase, and inhibited cell apoptosis. However, up-regulation of miR-10167-3p inhibited cell proliferation and promoted apoptosis. Briefly, circADAMTS16 inhibited the differentiation and promotes the proliferation of bovine adipocytes by targeting miR-10167-3p during bovine fat deposition, which provides new insights into the mechanism of circRNAs regulation of beef quality.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

Xue

et al. 2023

Cells

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“…The highest expression levels of circ‐0001122 and circ‐007367 are found in the bovine mammary gland, but they differ in other tissues (Feng, Cai, et al, 2022). The expression of circMARK3 in fat is higher than that of other tissues, and it aids in the differentiation of bovine adipocytes (Feng, Zhao, et al, 2022). The circTCF4 is expressed at a level that is highest in the gastrocnemius, followed by skeletal muscle, and at a level that is lowest in the liver.…”

Section: Basic Knowledge Of Circrnasmentioning

confidence: 99%

The emerging roles of circRNAs in traits associated with livestock breeding

et al. 2023

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Many indicators can be used to evaluate the productivity and quality of livestock, such as meat and milk production as well as fat deposition. Meat and milk production are measures of livestock performance, while fat deposition affects the taste and flavor of the meat. The circRNAs, are non-coding RNAs, that are involved in the regulation of all these three traits. We review the functions and mechanisms of circRNAs in muscle and fat development as well as lactation to provide a theoretical basis for circRNA research in animal husbandry. Various phenotypic changes presented in livestock may be produced by different circRNAs. Our current concern is how to use the roles played by circRNAs to our advantage to produce the best possible livestock. Hence, we describe the advantages and disadvantages of knockout techniques for cir-cRNAs. In addition, we also put forward our thoughts regarding the mechanism and network of circRNAs to provide researchers with novel ideas of how molecular biology can help us advance our goals in animal farming.

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“…However, the low IMF content of yak meat has become a major bottleneck, restricting high-quality meat development in the yak industry. Recent studies report that non-coding RNAs (ncRNAs) regulate key aspects of lipid metabolism [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

Qin,

Wang,

Zhong

et al. 2024

IJMS

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miR-129 plays a crucial role in regulating various cellular processes, including adipogenesis; however, its downstream molecular mechanisms remain unclear. In this study, we demonstrated that miR-129 promotes yak adipogenesis in vitro via the PI3K/AKT pathway. Overexpression and interference of miR-129 in yak intramuscular preadipocytes (YIMAs) enhanced and inhibited cell differentiation, respectively, with corresponding changes in cell proliferation. Further investigation revealed that miR-129 enhances AKT and p-AKT activity in the AKT pathway without affecting cell apoptosis, and a specific inhibitor (LY294002) was used to confirm that miR-129 regulates YIMAs proliferation and differentiation through the PI3K/AKT pathway. Our findings suggest that miR-129 promotes yak adipogenesis by enhancing PI3K/AKT pathway activity. This study provides the foundation to precisely elucidate the molecular mechanism of miR-129 in YIMAs adipogenesis and develop advanced miRNA-based strategies to improve meat nutrition and obesity-related ailments in beef production.

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Weighted Gene Co-expression Network Analysis Revealed That CircMARK3 Is a Potential CircRNA Affects Fat Deposition in Buffalo

Cited by 6 publications

References 61 publications

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

CircADAMTS16 Inhibits Differentiation and Promotes Proliferation of Bovine Adipocytes by Targeting miR-10167-3p

The emerging roles of circRNAs in traits associated with livestock breeding

miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

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