Weitreichender Elektronentransfer durch DNA-Filme

Sämtliche menschlichen Gene (ca. 100 000) sowie auch das ganze Spektrum der biologischen Vielfalt werden demnächst durch die Verwendung von DNA‐Mikroarrays simultan analysierbar sein, wenn die rasante Technikentwicklung auf diesem Gebiet weiterhin anhält. Die besondere Stärke der Array‐Analytik, die typischerweise auf der Hybridisierung Mikrochip‐gebundener Nucleinsäure‐Sonden mit markierter Proben‐RNA oder ‐DNA beruht, liegt dabei in der hochredundanten Messung vieler paralleler Hybridisierungsereignisse (siehe Bild), was eine hohe Nachweissicherheit gewährleistet.

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DNA-Mikroarrays

Niemeyer

Blohm

1999

Angewandte Chemie

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Wie bewegen sich Ladungen durch DNA? – Ein Zwischenbericht

Grinstaff¹

1999

Angewandte Chemie

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Der Einfluß von Oligonucleotidsequenz, Basenpaarung, Basenstapelung und Mikrostruktur des Doppelstranges ebenso wie der Donor‐Acceptor‐Abstand, die Markierungsposition und die energetischen Verhältnisse sind bei der Untersuchung von Ladungstransferreaktionen in DNA von Interesse. Bisher wurden allerdings nur wenige Experimente durchgeführt, die sich mit diesen Aspekten befassen. In diesem Beitrag werden aktuelle Ergebnisse auf dem Gebiet der DNA‐vermittelten Ladungsübertragung zusammengefaßt und Fragen zu Experiment und Theorie herausgearbeitet, die bislang noch unbeantwortet sind.

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DNA On Silicon Devices: On-Chip Synthesis, Hybridization, and Charge Transfer

et al. 2002

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Surface-immobilized DNA has an increasing number of roles in science and technology because of the ease with which it can be manipulated chemically and enzymatically in a highly controlled manner. Complex DNA architectures [1] and the use of DNA as a scaffold for making electronic connections [2] have been reported, and the use of DNA as a component in so-called molecular electronics has been frequently advocated. [3] Applications also include DNA microarrays [4] for sequencing through hybridization, the study of gene expression, and even for prototype computing devices which utilize the fidelity of the hybridization reaction. [5±7] In most cases, the DNA is immobilized on glass, oxidized silicon wafers, or other insulating supports. Methods for combining DNA with electronic materials are anticipated to be increasingly important in light of the considerable interest in DNAbased nanotechnology, [1] DNA-mediated charge transfer, [8] and DNA-based wires. [2] For substrates such as gold [9] or Si, [10] where there is the possibility of charge transfer through the DNA to the surface, immobilization has relied upon the attachment of presynthesized strands. However, to date there has been no demonstration of the use of automated solidphase DNA synthesis nor charge transfer through DNAbased assemblies at a semiconductor. For many proposed applications the ability to combine microelectronic processing techniques, [11] for example, photolithography and micromachining, with automated chemical synthesis has clear advantages. Herein we outline a method that enables the straightforward integration of DNA technology with microelectronics.Hydrogen-terminated surfaces represent the initial state from which silicon semiconductor devices are fabricated. [11] Chidsey and co-workers first demonstrated that such singlecrystal surfaces [12] react with alkenes to give chemically robust Si À C-bonded monolayers, and further advances have shown the applicability of this chemistry to porous silicon. [13±15] The reaction may be driven by thermal, catalytic, electrochemical, and photochemical methods, [13,16] and patterning of the monolayers, for example, by using photolithographic techniques, is possible. We have used a simple protecting group method to produce monolayers with terminal OH groups ( Figure 1); hydrogen-terminated silicon surfaces of oriented (111) single-crystal and porous silicon were alkylated with 4,4'-dimethoxytrityl-protected w-undecenol (trityl triphenylmethyl) by refluxing it in toluene; this is a previously

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Weitreichender Elektronentransfer durch DNA-Filme

Cited by 43 publications

References 53 publications

DNA-Mikroarrays

DNA-Mikroarrays

Wie bewegen sich Ladungen durch DNA? – Ein Zwischenbericht

DNA On Silicon Devices: On-Chip Synthesis, Hybridization, and Charge Transfer

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