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Tanihara, Isao; Ong, H. J.; Tamii, A.; Kishimoto, T.; Kajino, Toshitaka; Kubono, S.; Shima, T.

doi:10.1063/1.3485208

Cited by 2 publications

(4 citation statements)

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“…Some studies have defined the following electrospinning parameters for silk fibroin: flow rate of 0.5 mL/h [59], voltage of 15 kV [53], [59], and a distance of 15 cm between the needle and the collector [60]. The most widely employed collectors are rotating drum, flat sheet of aluminum foil, and spinneret submerged in a methanol bath.…”

Section: Electrospinning Parameters For Fibroinmentioning

confidence: 99%

“…The samples were carefully removed from the foil and cut into strips with the following sizes (length x width): 3 mm x 3 mm [83]; 0.5 cm x 4 cm [43], [53], [56]; 50 mm x 5 mm [41], [69]; and 50 mm x 12 mm with a thickness of 0.30 mm ± 0.01 mm [49]; 30 mm x 10 mm with a thickness of 0.8 mm [52], [64]; and 10 mm x 50 mm [66], [70], [74], [78] with a thickness of 0,16 mm ± 0,01 mm [70], (60 ~ 110 μm) [66], (170-370 nm) [74]. Then, they were pasted on a square paper template and tested in a universal machine at room temperature and a relative humidity of 65% using a load cell of 10 N at a constant extension rate of 20 mm/min [43], [49], [60], [74], [83], [84]; 5 mm/min [69], [76]; 10 mm/min [ [53], [66], [74]; and 50 mm/min [85]. The gauge length was 20 mm [66], [84].…”

Section: Mechanical Properties -Tensile Strength (Ts)mentioning

confidence: 99%

“…The gauge length was 20 mm [66], [84]. The maximum tensile strength (MPa), Young's modulus (MPa), and elongation percentage (%) were determined based on the recorded force-elongation curve [37], [52], [53], [60], [64], [74], [76], [78], [84]- [86].…”

Section: Mechanical Properties -Tensile Strength (Ts)mentioning

confidence: 99%

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Characterization of Electrospun Silk Fibroin Scaffolds for Bone Tissue Engineering: A Review

Suaza¹,

Moncada²,

Orozco³

2020

TecnoL.

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Silk Fibroin (SF) is a natural polymer obtained from the Bombyx mori silkworm. It has been used in bone tissue engineering thanks to its favorable biocompatibility, adhesion, low biodegradability, and tensile strength properties. Electrospinning is a technique to develop nanofibers. It uses high voltages to convert polymer solutions into porous nanostructured scaffolds with a good ratio between superficial area and volume. In this paper, we examine the effect of the electrospinning parameters on fiber morphology once the spun fibers have been treated. In addition, we present different physicochemical characterizations of electrospun SF scaffolds such as their morphology (via Scanning Electron Microscopic—SEM—), crystalline structure (via Fourier Transform Infrared—FTIR—spectroscopy and X-Ray Diffraction—XRD—), thermal characteristics (via Differential Scanning Calorimetry—DSC—and Thermogravimetric Analysis—TGA—), and mechanical properties (tensile strength). Finally, we discuss the potential applications and impacts of electrospun SF in bone tissue engineering and future research trends.

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Section: Electrospinning Parameters For Fibroinmentioning

confidence: 99%

Section: Mechanical Properties -Tensile Strength (Ts)mentioning

confidence: 99%

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Characterization of Electrospun Silk Fibroin Scaffolds for Bone Tissue Engineering: A Review

Suaza¹,

Moncada²,

Orozco³

2020

TecnoL.

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“…It should be noted that this direction of change is consistent with compensation for or responsiveness to accumulation of glycerolipids known to occur when saposins' function is compromised at the lysosome, and a reported ligand for both GPR37L1 and GPR37 is prosaptide, an active fragment of prosaposin, which is known to be involved in lysosomal trafficking and biology. 12,31,32,33,34,35,36 Additionally, a protein-protein interaction network was generated for the list of proteins that were significantly differentially expressed (both up-and downregulated) (Fig. 1B).…”

Section: Proteomic Analysis Of Global Protein Changes In the Gpr37/gpr37l1 Double Knockout Mouse Brain Vs Wild-type (Wt) Mouse Brainmentioning

confidence: 99%

Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1

et al. 2020

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GPR37 and GPR37L1 are glia-enriched GPCRs that have been implicated in several neurological and neurodegenerative diseases. To gain insight into the potential molecular mechanisms by which GPR37 and GPR37L1 regulate cellular physiology, proteomic analyses of whole mouse brain tissue from wild-type (WT) versus GPR37/GPR37L1 double knockout (DKO) mice were performed in order to identify proteins regulated by the absence versus presence of these receptors (data are available via ProteomeXchange with identifier PXD015202). These analyses revealed a number of proteins that were significantly increased or decreased by the absence of GPR37 and GPR37L1. One of the most decreased proteins in the DKO versus WT brain tissue was S100A5, a calcium-binding protein, and the reduction of S100A5 expression in KO brain tissue was validated via Western blot. Co-expression of S100A5 with either GPR37 or GPR37L1 in HEK293T cells did not result in any change in S100A5 expression but did robustly increase secretion of S100A5. To dissect the mechanism by which S100A5 secretion was enhanced, cells co-expressing S100A5 with the receptors were treated with different pharmacological reagents. These studies revealed that calcium is essential for the secretion of S100A5 downstream of GPR37 and GPR37L1 signaling, as treatment with BAPTA-AM, an intracellular Ca 2+ chelator, reduced S100A5 secretion from transfected HEK293T cells. Collectively these findings provide a panoramic view of proteomic changes resulting from loss of GPR37 and GPR37L1 and also impart mechanistic insight into the regulation of S100A5 by these receptors, thereby shedding light on the functions of GPR37 and GPR37L1 in brain tissue.

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Welcome Address

Cited by 2 publications

References 0 publications

Characterization of Electrospun Silk Fibroin Scaffolds for Bone Tissue Engineering: A Review

Characterization of Electrospun Silk Fibroin Scaffolds for Bone Tissue Engineering: A Review

Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1

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