Western blot analysis of Leishmania infantum antigens using sera from pentamidine-treated dogs

Lasri, Sâadia; Sahibi, Hamid; Natami, Amale; Rhalem, Abdelkbir

doi:10.1016/s0165-2427(02)00262-3

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…Identification of cross‐reactive antigens using human sera from patients infected with several infectious diseases was previously described (Brito et al, 2000; Hoerauf et al, 1992; Isaza et al, 1997; Reed et al, 1987; Reed et al, 1990). However, the cross‐reactivity of antibodies from L. infantum‐ infected dog sera were tested only against homologous soluble antigens or L. donovani antigens, both from Old World Leishmania species (Carrera et al, 1996; Fernandez‐Perez et al, 2003; Lasri et al, 2003; Nieto et al, 1999; Rhalem et al, 1999; Vercammen et al, 2002). In this study, we demonstrated the cross‐reactivity of L. chagasi‐ infected dog sera with four different New World Leishmania antigenic panels.…”

Section: Discussionmentioning

confidence: 99%

Identification of Highly Specific and Cross‐Reactive Antigens of Leishmania Species by Antibodies from Leishmania (Leishmania) chagasi Naturally Infected Dogs

Vale

Fujiwara

Neto³

et al. 2009

Zoonoses and Public Health

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The Leishmania species present a genetic homology that ranges from 69 to 90%. Because of this homology, heterologous antigens have been used in the immunodiagnosis and vaccine development against Leishmania infections. In the current work, we describe the identification of species-specific and cross-reactive antigens among several New World Leishmania species, using symptomatic and asymptomatic naturally Leishmania chagasi-infected dog sera. Soluble antigens from five strains of New World Leishmania were separated by electrophoresis in SDS-PAGE and immunoblotted. Different proteins were uniquely recognized in the L. chagasi panel by either symptomatic or asymptomatic dog sera suggesting their use as markers for the progression of disease and diagnosis of the initial (sub-clinical) phase of the infection. Cross-reactive antigens were identified using heterologous antigenic panels (L. amazonensis strains PH8 and BH6, L. guyanensis and L. braziliensis). L. guyanensis panel showed the highest cross-reactivity against L. chagasi specific antibodies, suggesting that proteins from this extract might be suitable for the diagnosis of visceral canine leishmaniasis. Interestingly, the 51 and 97 kDa proteins of Leishmania were widely recognized (77.8% to 100%) among all antigenic panels tested, supporting their potential use for immunodiagnosis. Finally, we identified several leishmanial antigens that might be useful for routine diagnosis and seroepidemiological studies of the visceral canine leishmaniasis.

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Section: Discussionmentioning

confidence: 99%

Identification of Highly Specific and Cross‐Reactive Antigens of Leishmania Species by Antibodies from Leishmania (Leishmania) chagasi Naturally Infected Dogs

Vale

Fujiwara

Neto³

et al. 2009

Zoonoses and Public Health

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“…There has always been a drive to find other drugs as an alternative to antimonials in the treatment of canine visceral leishmaniasis. Pentamidine, buparvaquone, enrofloxacin, metronidazole, spiramycin and ketoconazole have been tried, 32,37,51–54 but as of yet nothing has proven as effective as meglumine antimoniate. In fact, from the data collected in this review, there is only fair evidence for recommending the use of pentamidine and insufficient evidence for recommending the use of buparvaquone, ketoconazole, enrofloxacin and the combinations metronidazole/spiramycin and metronidazole/enrofloxacin.…”

Section: Discussionmentioning

confidence: 99%

Treatment of canine Old World visceral leishmaniasis: a systematic review

Noli¹,

Auxilia

2005

Veterinary Dermatology

152

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Canine visceral leishmaniasis is a systemic disease caused by Leishmania infantum. The aim of this systematic review was to identify and evaluate the evidence of efficacy of interventions for treatment or prevention of canine visceral leishmaniasis, and to propose recommendations for or against their use. Forty-seven articles describing clinical trials published between 1980 and 2004 fulfilled selection criteria. The evaluation of clinical trials provided good evidence for recommending the use of meglumine antimoniate at a minimum dosage of 100 mg kg(-1) daily for at least 3-4 weeks, combined with allopurinol in order to obtain a good clinical efficacy and a reduced relapse rate. The evaluation of the articles also provided fair evidence for recommending the use of pentamidine (4 mg kg(-1) twice weekly) and aminosidine (5 mg kg(-1) twice daily) for 3-4 weeks. There was insufficient evidence for recommending the use of allopurinol alone, amphotericin B, buparvaquone, ketoconazole, enrofloxacin, and the combinations of metronidazole with spiramicyn or metronidazole with enrofloxacin. Fair evidence against the use of aminosidine at high dosages (20-80 mg kg(-1) per day) was proposed due to its side effects. Evaluation of articles on repellent measures against sand fly vectors of leishmaniasis provided good evidence for recommending deltamethrin collars and fair evidence for recommending spot-on permethrin.

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“…Susceptible dogs produce antibodies against a wide range of parasitic antigens such as K39, heat shock proteins (HSP‐70), histones (H‐2A, H‐2B, H‐3, and H‐4), cysteine proteinases (CPA and CPB), proteins gp63 and gp70, O ‐acetylated sialic acids and ribosomal P proteins (P0, P2a, P2b) 23,27,126,184–193 . The particular pathogenetic role of each of these antigen‐specific antibodies is not known, although seropositivity to K39 antigen and to protein gp63 have been linked to the appearance of clinical signs 137,192 …”

Section: Immunology Of Canine Leishmaniosismentioning

confidence: 99%

Advances in the pathogenesis of canine leishmaniosis: epidemiologic and diagnostic implications

Saridomichelakis

2009

Veterinary Dermatology

117

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Canine leishmaniosis caused by Leishmania infantum (Syn. L. chagasi) is an important zoonosis with a complex pathogenesis. Parasite transmission occurs via female sandflies that inject promastigotes into the skin of the host. The interaction between the parasite and skin immune system is influenced by the repeated infectious bites and the simultaneous intradermal injection of sandfly saliva. Amastigotes are transported via infected macrophages to the regional lymph nodes and finally dissemination may occur. The outcome of the infection depends on host factors (genetic background, cell-mediated and humoral immune response, cytokine milieu, concurrent diseases) and parasite virulence. Resistance may be breed-associated; it is characterized by low to undetectable antibody production and effective cell-mediated immunity, and is orchestrated by cytokines such as interleukin-2, interferon-gamma and tumour necrosis factor-alpha. Susceptibility may be genetically determined or acquired (advanced age, concurrent diseases); in these dogs, parasite multiplication goes unrestricted and overproduction of specific and nonspecific antibodies occurs, leading to multiple organ pathology. Resistance or susceptibility is not an all-or-nothing phenomenon and many intermediate phenotypes may be found. From a diagnostic point of view, although clinical cases are readily identified using microscopy and serology, investigation should not stop at this point and an extensive search for underlying diseases is advised, especially in aged dogs. Conversely, microscopy and conventional serology are frequently negative in asymptomatic infected dogs; to identify such dogs, polymerase chain reaction, evaluation of cutaneous delayed-type hypersensitivity, in vitro lymphocyte proliferation test to Leishmania antigen, and Western blotting may be employed.

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Western blot analysis of Leishmania infantum antigens using sera from pentamidine-treated dogs

Cited by 8 publications

References 15 publications

Identification of Highly Specific and Cross‐Reactive Antigens of Leishmania Species by Antibodies from Leishmania (Leishmania) chagasi Naturally Infected Dogs

Identification of Highly Specific and Cross‐Reactive Antigens of Leishmania Species by Antibodies from Leishmania (Leishmania) chagasi Naturally Infected Dogs

Treatment of canine Old World visceral leishmaniasis: a systematic review

Advances in the pathogenesis of canine leishmaniosis: epidemiologic and diagnostic implications

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