Wet-spun silk fibroin scaffold with hierarchical structure for ligament tissue engineering

Wu, Hui; Zhang, Feng; Yue, Xiao X.; Ming, Jin; Zuo, Baoqi

doi:10.1016/j.matlet.2014.07.115

Cited by 11 publications

(4 citation statements)

References 17 publications

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“…It is assumed that the first description of a wet spinning process of regenerated silk fibers dates back to 1960, 14 in which an aqueous dope of fibroin was coagulated into a fiber by using a concentrated ammonium sulfate solution. Subsequent attempts relied on dopes that contained relatively harsh chemicals, such as orthophosphoric acid (H 3 PO 4 ), 15 formic acid 16,17 or hexafluoroacetate hydrate, 18 among others. [19][20][21][22] Usage of these solvents allowed high concentrations of silk protein in the solutions 23 to be reached, which was considered suitable for the efficiency of the spinning process.…”

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confidence: 99%

Production of regenerated silkworm silk fibers from aqueous dopes through straining flow spinning

Madurga

Gañán‐Calvo

Mariscal

et al. 2019

Textile Research Journal

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The possibility of spinning regenerated silkworm ( Bombyx mori) fibers from a range of aqueous dopes by using a biomimetic approach based on straining flow spinning is explored in this work. It is found that spinning conditions can be established that allow fibers to be produced from environmentally friendly dopes with fibroin concentrations even one order of magnitude lower than that of the natural system. However, it is also found that the spinning process is favored and that the mechanical properties of the fibers are improved when dopes with higher fibroin concentration are employed. Since highly concentrated fibroin solutions in water are unstable, a stabilizing agent is required in order to obtain a spinnable dope at such large protein concentrations. CaCl2 is found to be an adequate stabilizing agent compatible with the straining flow spinning process. The optimization of the spinning parameters leads to the production of high-performance fibers with a work to fracture comparable to that of the natural material.

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mentioning

confidence: 99%

Production of regenerated silkworm silk fibers from aqueous dopes through straining flow spinning

Madurga

Gañán‐Calvo

Mariscal

et al. 2019

Textile Research Journal

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“…40 Silk scaffolds can support the proliferation and gene expression of chondrocytes, which have a promising application in ligament tissue engineering. 41,42 Finally, the apatite mineralization is probably able to adsorb serum proteins and growth factors, which in turn stimulates cell proliferation and activates cell differentiation according to previous studies. 43,44 In this study, our results show that pure silk fibroin itself has no obvious apatite-formation ability.…”

Section: Discussionmentioning

confidence: 73%

Hierarchically porous nagelschmidtite bioceramic–silk scaffolds for bone tissue engineering

Zhai

et al. 2015

J. Mater. Chem. B

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Bioactive three-dimensional (3D) scaffolds play a key role in the repair or regeneration of large bone defects.There are many methods to prepare 3D scaffolds, among which the 3D-plotting technique is a promising strategy as the scaffolds prepared by this method possess not only improved mechanical properties and interconnectivity, but also ordered large-pore structure. However, the low cell attachment rate in the interior of the 3D-plotted scaffolds, especially for 3D-plotted bioceramic scaffolds, inhibits the osteogenesis of stem cells in the scaffolds both in vitro and in vivo. The aim of this study is to prepare hierarchically porous composite scaffolds in order to improve the cell attachment, and further stimulate the in vitro and in vivo osteogenesis. We successfully fabricated hierarchically porous bioceramic-silk (BC-silk) composite scaffolds by a combination of the 3D-plotting technique with the freeze-drying method, and further investigated the attachment, proliferation and osteogenic differentiation of bone marrow stromal cells (BMSCs) in the scaffolds as well as the in vivo osteogenesis of the prepared porous scaffolds. The results showed that the hierarchical structure in the composite scaffolds was composed of first-level pores (B1 mm) of the bioceramic scaffold and second-level pores (B50-100 mm) of the silk matrix. The prepared BC-silk composite scaffolds possessed excellent apatite-mineralization ability and mechanical properties with compressive strength up to 25 MPa. In addition, hierarchically porous BC-silk scaffolds presented significantly enhanced attachment rate of BMSCs, around 4 times that of pure BC scaffolds without hierarchical pore structures. BC-silk scaffolds with hierarchical pore structures showed distinctively improved cell proliferation, ALP activity and bone-related gene expression as compared to BC scaffolds without hierarchical pore structure. Furthermore, hierarchically porous BC-silk scaffolds significantly enhanced the formation of new bone in vivo as compared to BC scaffolds. Our results suggest that the combination of 3D-plotting with the freeze-drying method is a viable strategy to construct hierarchical pore structures in 3D-plotted scaffolds, and the hierarchical pore structure plays an important role in improving the in vitro and in vivo osteogenesis of 3D-plotted bioceramic scaffolds for bone regeneration application.

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“…Biodegradation tests showed that such matrices lose 8% of their weight after being placed into sodium phosphate buffer for 60 days and 62% of their weight when in a solution of actinomycete proteases for 48 hours. The authors [138] concluded that their hierarchical structure, mechanical properties, high biocompatibility and biodegradation resistance meant that matrices of regenerated silk are appropriate for use in the tissue engineering of ligaments.…”

Section: Use Of Silk Fibroin and Spidroin In Tissue Engineering And Rmentioning

confidence: 99%

Silk Fibroin and Spidroin Bioengineering Constructions for Regenerative Medicine and Tissue Engineering (Review)

Agapova¹

2017

Sovrem Tehnol Med

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The review is about the developments of modern bioengineering constructs from two unique biopolymers: the main protein of silkworm silk, fibroin, and frame silk of spider web, spidroin, and their applications in regenerative medicine and tissue engineering. Both types of polymers possess such important properties as biocompatibility, biodegradability, high strength and elasticity. Availability of silkworm cocoons in nature, debugged methods of fibroin purification make this protein very perspective in bioengineering constructs. A spider web protein, spidroin, is less common in nature, but the development of alternative methods for its production makes it a promising biopolymer.The structure and properties of silk fibroin and spidroin, their advantages over other natural and synthetic polymers, technologies of biopolymer construct fabrication are considered in this review. Silk fibroin and spidroin are shown to be applied for creation of 3D matrices promoting regeneration of damaged organs and tissues, biodegradable cell carriers and pharmaceutical preparations.

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Wet-spun silk fibroin scaffold with hierarchical structure for ligament tissue engineering

Cited by 11 publications

References 17 publications

Production of regenerated silkworm silk fibers from aqueous dopes through straining flow spinning

Production of regenerated silkworm silk fibers from aqueous dopes through straining flow spinning

Hierarchically porous nagelschmidtite bioceramic–silk scaffolds for bone tissue engineering

Silk Fibroin and Spidroin Bioengineering Constructions for Regenerative Medicine and Tissue Engineering (Review)

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