Disturbance is an important factor influencing plant species composition and diversity. We addressed changes in plant composition and soil characteristics in Estero de Punta Banda, Baja California, Mexico following 22 years of disturbance by tidal exclusion. Currently, sediments in the non-tidal site are dry, 26±1% moisture, and hypersaline, 143±12; while those at the tidal marsh are wet, 36.2±1% moisture, with 40.3±2.6 salinity. The nontidal site has lost seven species including annuals, shortand long-lived perennials, ephemerals, and parasites. Current dominants are the perennials Batis maritima and Sarcocornia pacifica. Average species richness at the nontidal site is 4.4±0.32 vs.10±0.18 species per square meter at the tidal site. Average species diversity index is lower at the diked area. The general biodiversity loss that results from tidal exclusion in arid estuaries, contrasts with the species-rich communities that develop in diked humid-climate estuaries.