Weybridge P.P.D. Tuberculins

Green, H.H.

doi:10.1016/s0372-5545(17)31352-4

Cited by 26 publications

(8 citation statements)

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“…The following antigens were evaluated: i) M. bovis tuberculin PPD, batch 1-85,5.5 mg/ml in pH 7.0 phosphate buffered saline (PBS), 0.5 YO phenol solution, prepared at CEPANZO from autoclaved strain A N 5 culture filtrates on Dorset Henley medium, by the trichloroacetic acid precipitation method according to GREEN and modified by H. HUITEMA (8,11). This PPD was standardized against the European Economic Community Standard for bovine PPD in guinea pigs (20).…”

Section: Antigensmentioning

confidence: 99%

Assessment of the Sensitivity and Specificity of Enzyme‐Linked Immunosorbent Assay (ELISA) for the Detection of Mycobacterial Antibodies in Bovine Tuberculosis

Ritacco

Kantor

Barrera

et al. 1987

Journal of Veterinary Medicine, Series B

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Summary An ELISA which detects bovine circulating IgG mycobacterial antibodies using M. bovis PPD as antigen was assessed. PPDs prepared from unheated cultures of two M. bovis strains were compared with autoclaved bovine PPD; the latter was found to be a more reliable antigen. Ninety per cent of bacteriologically confirmed tuberculous cattle were detected by this method (18/20), whereas 89.8% of negative reactions (44/49) were observed in healthy cattle from a tuberculosis‐free area. Antibody levels of tuberculin negative cattle from an endemic area did not differ significantly from antibody levels of cattle from a tuberculosis‐free area. An almost complete qualitative correlation was observed between instrumental and visual readings. In the present trial, the sensitivity and specificity of the ELISA were similar to those of the tuberculin skin test at its best. In addition, operative advantages and low cost of the ELISA make of it a valuable tool for the diagnosis of bovine tuberculosis. Zusammenfassung Bestimmung der Sensitivität und Spezifität eines enzymgebundenen Immunadsorptionstests (ELISA) zum Nachweis von mykobakteriellen Antikörpern bei boviner Tuberkulose Ein ELISA zum Nachweis von zirkulierenden, bovinen IgG‐Antikörpern mit M. bovis als Antigen wurde überprüft. Autoklaviertes bovines PPD erwies sich, verglichen mit PPDs aus nicht erhitzten Kulturen zweier M. bovis‐Stämme, als geeignetes Antigen. Mit Hilfe dieser Methode konnten bei Rindern mit bakteriologisch abgesicherter Tuberkulose in 90% der Fälle (18/20) Antikörper nachgewiesen werden. Negative Befunde wurden dagegen zu 89,9% bei gesunden Tieren aus einem tuberkulosefreiem Gebiet ermittelt. Zwischen den Antikörpergehalten tuberkulinnegativer Rinder aus einem endemischen Gebiet und Tieren aus dem tuberkulosefreien Gebiet konnte keine signifikante Abweichung festgestellt werden. Eine nahezu vollständige Korrelation bestand zwischen instrumenteller und visueller Ablesung. In der vorliegenden Untersuchung waren die Ergebnisse des ELISA bezüglich Sensitivität und Spezifität ähnlich denen des Tuberkulin‐Hauttestes. Darüber hinaus machen die technischen Vorteile und geringen Kosten den ELISA zu einem nützlichen Hilfsmittel für die Diagnostik der bovinen Tuberkulose.

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Section: Antigensmentioning

confidence: 99%

Assessment of the Sensitivity and Specificity of Enzyme‐Linked Immunosorbent Assay (ELISA) for the Detection of Mycobacterial Antibodies in Bovine Tuberculosis

Ritacco

Kantor

Barrera

et al. 1987

Journal of Veterinary Medicine, Series B

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“…In these studies the method used by Green (1946), as modified in the Connaught MViedical Research Laboratories, was shown to be suitable for the production of tuberculin PPD. Long's synthetic medium (Long and Seibert, 1926) replaced the medium employed by Green (1946), and only one strain of M. tuberculosis, the "Johnston" strain, was used instead of the three strains, C., D.T., and P.N.…”

Section: Resultsmentioning

confidence: 99%

Preparation, Purification, and Stability of Tuberculin

Landi

1963

Applied Microbiology

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Preparation, purification, and stability of tuberculin. Appl. Microbiol. 11:408-412. 1963.-The method used to produce "Connaught" tuberculin purified protein derivative (PPD) is described. The tuberculin PPD for the multiplepuncture method was shown to be stable for at least 24 months at 5 C; tuberculin PPD for the intracutaneous method was shown to be stable at 5 C and 24 C for a period of 18 months in the presence of Tween 80. Evans blue or brillant vital red was added to tuberculin PPD for improved testing by the multiple-puncture method. These tinted tuberculin preparations were found to be as stable as the Connaught tuberculin PPD preparations without dye at 5 C. Freeze-dried tuberculin PPD with Plasdone as an inert base was found to be remarkably stable for a period of at least 24 months at 5, 24, and 37 C.

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“…Such a test is not yet available with the antigenic preparations on hand. Some tuberculin fractions (such as Seibert's fraction "A", "B" or "Bac II"1,2 and other products 3 ), have been demonstrated to elicit greater sensitivity and specificity than conventional purified protein derivative (PPD).4 However, their improvement over conventional PPD preparations has not been sufficiently demonstrated to stimulate widespread use or development of techniques to make them commercially available. Although a more sensitive and specific antigen must be developed, there is not adequate quantitative data to reveal the true potential of PPD prepared from Submitted for publieation Dec 26, 1967 infection.…”

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confidence: 99%

New Aspects of Mycobacterial Skin Tests

Vakilzadeh

et al. 1968

Archives of Environmental Health: An International Journal

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Weybridge P.P.D. Tuberculins

Cited by 26 publications

References 0 publications

Assessment of the Sensitivity and Specificity of Enzyme‐Linked Immunosorbent Assay (ELISA) for the Detection of Mycobacterial Antibodies in Bovine Tuberculosis

Assessment of the Sensitivity and Specificity of Enzyme‐Linked Immunosorbent Assay (ELISA) for the Detection of Mycobacterial Antibodies in Bovine Tuberculosis

Preparation, Purification, and Stability of Tuberculin

New Aspects of Mycobacterial Skin Tests

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