Wheat anther culture

Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.)

“…Their ovary conditioned medium, however, was diluted with fresh induction medium prior to use. Despite significant recent advances in wheat microspore culture Kasha et al 2003), anther culture remains a Plant Cell Tiss Organ Cult (2008) 95:185-195 191 viable option for the routine production of doubled haploids (Barnabás 2003;Pauk et al 2003, Tuvesson et al 2003 because of its technical simplicity. The results from this study indicate that the addition of as few as five ovaries per dish can have a dramatic impact on the production of ELS and green plants in the anther culture of Australian spring wheat genotypes.…”

“…Maltose has been demonstrated to be superior to sucrose as a carbohydrate source for cereals (Hunter 1988) and is generally preferred for induction in wheat anther and microspore culture, with either maltose or sucrose employed at the regeneration phase. Common growth regulator combinations used during the induction phase of wheat anther culture include 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) (Barnabás 2003;Pauk et al 2003;Tuvesson et al 2003) whereas the auxin, phenylacetic acid (PAA), has often been preferred for microspore culture (Hu et al 1995;Liu et al 2002;Kasha et al 2003). The combination of indole-3-acetic acid (IAA) and 6-benzyladenine (BA) has been used in some barley protocols (Logue et al 1993;Davies and Morton 1998) but has not generally been used for wheat.…”

Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum L.) breeding programmes

“…Other researchers have increased the efficiency of anther culture using responsive genotypes in the breeding programmes (Tuvesson et al. , ). However, this strategy would require more experiments before the production of DHs for breeding.…”

“…This involved prescreening genotypes, and the criterion for each cross was that one of the parental genotypes should produce at least one green plantlet/spike (Tuvesson et al. ). Other programmes also recommend the application of responsive genotypes in the breeding programmes based on anther culture (Kondic‐Spika et al.…”

“…To avoid this problem, one strategy is to use responsive breeding material in crossing to improve the efficiency of wheat anther culture, as recommended by Tuvesson et al (2000). This involved prescreening genotypes, and the criterion for each cross was that one of the parental genotypes should produce at least one green plantlet/spike (Tuvesson et al 2003). Other programmes also recommend the application of responsive genotypes in the breeding programmes based on anther culture (Kondic-Spika et al 2011).…”