Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight

Rawat, Nidhi; Pumphrey, Michael O.; Liu, Sixin; Zhang, Xiaofei; Tiwari, Vijay K.; Ando, Kaori; Trick, Harold N.; Bockus, W. W.; Akhunov, Eduard; Anderson, James A.; Gill, Bikram S.

doi:10.1038/ng.3706

Cited by 286 publications

(245 citation statements)

References 36 publications

Supporting

Mentioning

228

Contrasting

Order By: Relevance

“…Notably, the Rht-D1b allele had a more significant effect on anther retention and FHB severity than the Rht-B1b allele suggesting that differences in disease severity associated with the two semi-dwarfing alleles can be partly explained by their different effect on anther extrusion. A partly common genetic control of anther extrusion and plant First FHB resistance QTL is cloned (Rawat et al 2016) Initial utilization of molecular markers for selection (Del Blanco et al 2003;Yang et al 2003;Zhou et al 2003) Phenotypic selection is the only option 1999 First FHB resistance QTL are mapped (Waldron et al 1999;Bai et al 1999) Applicability of genomic selection is demonstrated (Rutkoski et al 2012) 2003 2012 2016…”

Section: Morphological and Phenological Traitsmentioning

confidence: 99%

Breeding strategies and advances in line selection for Fusarium head blight resistance in wheat

Steiner

Buerstmayr

Michel

et al. 2017

Trop. plant pathol.

152

155

View full text Add to dashboard Cite

Fusarium head blight (FHB) is a fungal disease of worldwide importance to small grain cereals that may lead to severe losses in both yield and quality. The development of resistant varieties is the most effective approach for managing the disease. Genetic variation for FHB resistance is large, including 'exotic' and 'native' wheat germplasm. Methods for selecting improved lines include: 1) phenotypic selection with direct symptom evaluation; 2) marker-assisted selection for well-characterized QTL and 3) genomic selection employing genome-wide prediction models. Breeding programs need to find the optimal deployment of the complementary approaches according to their available facilities, resources and requirements. This review aims to summarize recent advances in FHB resistance breeding, thereby discussing the importance of morphological traits like the extent of retained anthers after flowering, its suitability for indirect selection and the pronounced association of the semi-dwarfing allele Rht-D1b with increased anther retention and FHB severity. Markerassisted selection is successfully applied to select for largeeffect QTL, especially for the most prominent resistance QTL Fhb1 in bread wheat, as well as in durum wheat as recently demonstrated. The resistance locus Fhb1 has been partly elucidated, a pore-forming toxin-like gene confers resistance against fungal spread. Genomic selection for FHB resistance appears promising especially for breeding programs deploying 'native' resistance sources with many small-effect QTL.

show abstract

Section: Morphological and Phenological Traitsmentioning

confidence: 99%

Breeding strategies and advances in line selection for Fusarium head blight resistance in wheat

Steiner

Buerstmayr

Michel

et al. 2017

Trop. plant pathol.

152

155

View full text Add to dashboard Cite

show abstract

“…Although a reference genome sequence can serve as a 'guide' to narrow down the location of a gene, the gene causing the phenotype of interest is often absent from the reference cultivar 1,4 which means that sequence information from a line that carries the gene of interest is needed. Repeated rounds of bacterial artificial chromosome (BAC) library screening, or chromosome walking, are usually necessary to cover the region of interest with a contiguous sequence 8 .…”

mentioning

confidence: 99%

Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

et al. 2017

View full text Add to dashboard Cite

Cereal crops such as wheat and maize have large repeat-rich genomes that make cloning of individual genes challenging. Moreover, gene order and gene sequences often differ substantially between cultivars of the same crop species. A major bottleneck for gene cloning in cereals is the generation of high-quality sequence information from a cultivar of interest. In order to accelerate gene cloning from any cropping line, we report 'targeted chromosome-based cloning via long-range assembly' (TACCA). TACCA combines lossless genome-complexity reduction via chromosome flow sorting with Chicago longrange linkage to assemble complex genomes. We applied TACCA to produce a high-quality (N50 of 9.76 Mb) de novo chromosome assembly of the wheat line CH Campala Lr22a in only 4 months. Using this assembly we cloned the broad-spectrum Lr22a leaf-rust resistance gene, using molecular marker information and ethyl methanesulfonate (EMS) mutants, and found that Lr22a encodes an intracellular immune receptor homologous to the Arabidopsis thaliana RPM1 protein.DOI: https://doi.org/10.1038/nbt.3877Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-144182 Journal Article Accepted Version Originally published at: Thind, Anupriya Kaur; Wicker, Thomas; Šimková, Hana; Fossati, Dario; Moullet, Odile; Brabant, Cécile; Vrána, Jan; Doležel, Jaroslav; Krattinger, Simon G (2017). Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly. Nature Biotechnology, 35(8) Using this assembly we cloned the broad-spectrum Lr22a leaf-rust resistance gene using molecular marker information and EMS mutants and found that Lr22a encodes an intracellular immune receptor homologous to the Arabidopsis RPM1 protein.While the world population continues to grow, the arable land per capita is decreasing 6 . To ensure food security, agriculture will require high-yielding crops that can withstand diseases, pests and adverse climatic conditions. A better understanding of genes that control these important traits may enable breeding of crop cultivars capable of feeding the 9-10 billion people expected by 2050. triticina isolates [13][14][15][16] . The Lr22a-mediated resistance is not present in young seedlings (<20 days) but is only visible in wheat plants from ~25 days of age.First, in order to evaluate the effectiveness of Lr22a against Swiss P. triticina isolates we inoculated the Lr22a-containing backcross line RL6044 ('Thatcher Lr22a') and the spring wheat cultivar 'Thatcher' with ten P. triticina isolates that were collected in Switzerland. The first leaves of RL6044 developed leaf rust pustules of similar size as the susceptible control 'Thatcher', while we observed complete to moderate resistance on the third leaves of 30-dayold RL6044 plants in comparison to 'Thatcher' (Fig. 1, Supplementary Fig. 1).Lr22a was previously mapped to the short arm of wheat chromosome 2D using microsatellite analysis of the Lr22a-containing wheat line 98B34-T4B 13 . In order to...

show abstract

“…Previous studies showed that Sumai3 and Wangshuibai carried the well-known major resistance QTL Fhb1 with the strongest FHB resistance (Cuthbert et al 2006;Rawat et al 2016). Since the use of Sumai3 in breeding is still unsuccessful (Chen et al 2012), breeders appreciate other germplasm of resistance with some improved and important traits.…”

Section: Discussionmentioning

confidence: 99%

Identification of three new resources of resistance to Fusarium head blight in wheat

Huang¹,

Fatima²,

Zhong³

et al. 2019

CZECH J GENET PLANT

View full text Add to dashboard Cite

Fusarium head blight (FHB) mainly caused by Fusarium species is one of the most important diseases threatening worldwide wheat production. To develop new FHB resistance resources, disease resistances of three new wheat lines L958, L962 and L987 were evaluated over a period of several years. We employed L699 (PI672540) and L661 as the resistant and susceptible control, respectively. Moderate FHB resistance was observed in these three wheat lines, among which the genotype L958 was found a good resource for yield, quality and FHB resistance improvement. In addition, genotype L962 was found to be resistant to powdery mildew, and both L958 and L987 carried the YrL693 gene conferring stripe rust resistance. The study provides promising results for accelerating the resistance breeding of the three wheat lines. Keywords: agronomic trait; FHB; resistance evaluation; Triticum aestivumFusarium head blight (FHB), mainly caused by Fusarium graminearum Schwabe and F. culmorum WG Smith, is a serious wheat (Triticum aestivum L.) disease worldwide (Bai & Shaner 2004;Li et al. 2017). In China, FHB epidemics have frequently occurred in the middle to lower valleys of the Yangtze River (Yao & Lu 2000). In recent years, FHB has spread to the southwest of China, including Sichuan Province (Zhang et al. 2011;Liu et al. 2015;Yang et al. 2016), where the climate was warm and rainy. Furthermore, FHB is also a fungal disease threatening food and economic security due to its contamination with mycotoxins, decreasing the wheat yield and quality (Zwart et al. 2008). At present, there is still no economic and effective way to control this disease. Developing resistant cultivars is an important approach to control this disease, for which the decisive step is to identify new germplasm with multi-resistance.Some effective FHB resistance sources have been identified in common wheat (Chu et al. 2011), and some FHB resistance quantitative trait loci (QTLs) have also been found and mapped in wheat relatives (Cai et al. 2005;Chen et al. 2007). In recent years, some geneticists and breeders have paid much ata These authors contributed equally to this work. 16 Original PaperCzech Journal of Genetics and Plant Breeding, 55, 2019 (1) (Luo et al. 2009;Liu et al. 2013). In addition, four new FHB resistant wheat germplasms were identified and released , which demonstrated the possibility of using Th. intermedium to develop novel wheat germplasm with different disease resistance. However, some breeding problems still remain and new materials with effective resistance to various diseases need to be developed.The research in this paper is aimed at screening wheat FHB resistance germplasm derived from an MY11 × YU25 cross for FHB resistance improvement. MATERIAL AND METHODSPlant material. The three wheat lines L958, L962, and L987 were selected from the F 7 populations derived from an MY11 × YU25 cross during 2007. MY11 is a wheat cultivar widely grown in southwestern China, while YU25 is a wheat cultivar with a foreign pedigree of Th. intermedium .FH...

show abstract

Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight

Cited by 286 publications

References 36 publications

Breeding strategies and advances in line selection for Fusarium head blight resistance in wheat

Breeding strategies and advances in line selection for Fusarium head blight resistance in wheat

Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

Identification of three new resources of resistance to Fusarium head blight in wheat

Contact Info

Product

Resources

About