Wheat-root associated prokaryotic community: interplay between plant selection and location

Abstract

Background Root-associated microbiomes are important for plant nutrient uptake, disease suppression and plant growth. It is important to reveal wheat-root associated microbial community assembly and dominant drivers determining their variability. Methods Using 16S rRNA gene profiling, we investigated the effects of sample type, location, growth stage and variety on prokaryotic communities in the root endosphere and rhizosphere of wheat and bulk soil based on the field samples including 5 varieties from 4 locations along similar latitude with the distance about 157 to 800 km apart between any two locations.Results Prokaryotic communities were more diverse in the bulk soil and rhizosphere than in root endosphere. Wheat-root associated prokaryotic community assembly was shaped predominantly by sample type, while within each sample type, location had stronger effects on the variation in prokaryotic community than growth stage or variety. Wheat variety effects varied substantially among different locations and growth stages in root endosphere and rhizosphere samples, and the variety effects were location-specific and growth stage-specific. Root endosphere specially enriched Pseudomonas, relative to other two sample types, while rhizosphere mainly enriched Bacillus. Conclusions This study characterized prokaryotic communities of wheat-root endosphere and rhizosphere and their relationships, and demonstrated significant interactive effects between wheat variety, location and growth stage on prokaryotic community assembly in field condition.

The effects of P on wheat root-associated prokaryotic communities are dependent on soil P content, growth stage and variety

“…1 and 2). This is consistent with the previous report (Zheng et al, 2021b) that even in eld grown wheat from different locations with large geographic distance, the compartment niche still has the greatest impact on root-associated microbial community.…”

“…It is worth noting that the contribution of growth stage to prokaryotic community variation was highest in endosphere, followed by rhizosphere, then by bulk soil, indicating that host growth selection gradually weakened from endosphere to bulk soil. Although previous study has shown that the growth stage has little effect on prokaryotic communities of bulk soil in the wheat eld (Zheng et al, 2021b), this work found that the prokaryotic communities of the bulk soil changed with growth stage, possibly because the regular nutrient input altered soil properties.…”

“…In agroecosystems and natural habitats, biotic and abiotic in uences can overwhelm, amplify or mask the in uence of host genes (Wagner et al, 2016). Compared to previous studies (Zheng et al, 2021b), this study was conducted in a controlled environment which eliminated the complex natural exogenous stimuli driving the phenotype, thus, more realistically re ected the impacts of different factors on wheat root microbiome.…”

“…Previous studies showed that compartment niche, growth stage, variety, and nutrient input all affect the root microbial community assembly of wheat. Zheng et al investigated the eld samples including 5 wheat varieties from 4 locations, and con rmed that wheat root-associated prokaryotic community assembly is shaped predominantly by sample type, and signi cant interactive effects occur among wheat variety, location and growth stage on prokaryotic community assembly (Zheng et al, 2021b). Chen et al investigated the compositions of bacterial communities in the roots and rhizosphere of wheat under four levels of long-term inorganic nitrogen (N) fertilization at the tillering, jointing and ripening stages.…”

Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

“…For example, the effect of maize on the rhizosphere microbial community varies depending on the location of the plant 21 . In addition, plants such as wheat 22 , tobacco 23 and tomato 24 exhibit the same situation. It has also been shown that bacterial wilt resistant and susceptible plants have different rhizosphere bacterial communities.…”