When multiphoton microscopy sees near infrared

Mojzisova, Halina; Vermot, Julien

doi:10.1016/j.gde.2011.08.004

Cited by 17 publications

(10 citation statements)

References 56 publications

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“…Second harmonic light can be generated when two photons with the same frequency interact with non-centrosymmetric structures and are effectively combined to generate a new photon with twice the energy (Mojzisova and Vermot, 2011). This approach allows muscle fibers imaging without any exogenous labeling at high resolution (Plotnikov et al, 2006).…”

Section: Desmin Loss or Aggregation Leads To Muscle Disorganization Amentioning

confidence: 99%

Developmental Alterations in Heart Biomechanics and Skeletal Muscle Function in Desmin Mutants Suggest an Early Pathological Root for Desminopathies

et al. 2015

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Desminopathies belong to a family of muscle disorders called myofibrillar myopathies that are caused by Desmin mutations and lead to protein aggregates in muscle fibers. To date, the initial pathological steps of desminopathies and the impact of desmin aggregates in the genesis of the disease are unclear. Using live, high-resolution microscopy, we show that Desmin loss of function and Desmin aggregates promote skeletal muscle defects and alter heart biomechanics. In addition, we show that the calcium dynamics associated with heart contraction are impaired and are associated with sarcoplasmic reticulum dilatation as well as abnormal subcellular distribution of Ryanodine receptors. Our results demonstrate that desminopathies are associated with perturbed excitation-contraction coupling machinery and that aggregates are more detrimental than Desmin loss of function. Additionally, we show that pharmacological inhibition of aggregate formation and Desmin knockdown revert these phenotypes. Our data suggest alternative therapeutic approaches and further our understanding of the molecular determinants modulating Desmin aggregate formation.

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Section: Desmin Loss or Aggregation Leads To Muscle Disorganization Amentioning

confidence: 99%

Developmental Alterations in Heart Biomechanics and Skeletal Muscle Function in Desmin Mutants Suggest an Early Pathological Root for Desminopathies

et al. 2015

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“…The simultaneous absorption of two photons allows, in the case of degenerate TPA, to divide the required energy by a factor of two, i.e., to double the vertical transition wavelength. This permit to notably shift the required incoming photon energy toward the red part of the visible spectrum (i.e., bathochromic shift), eventually allowing to enter in the so-called near-infrared therapeutic window (from 650 to 1350 nm), where the penetration of biological tissues is maximal (Tsai et al, 2001 ; Mojzisova and Vermot, 2011 ).…”

Section: Non-linear Spectroscopymentioning

confidence: 99%

Steady-State Linear and Non-linear Optical Spectroscopy of Organic Chromophores and Bio-macromolecules

et al. 2018

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Bio-macromolecules as DNA, lipid membranes and (poly)peptides are essential compounds at the core of biological systems. The development of techniques and methodologies for their characterization is therefore necessary and of utmost interest, even though difficulties can be experienced due to their intrinsic complex nature. Among these methods, spectroscopies, relying on optical properties are especially important to determine their macromolecular structures and behaviors, as well as the possible interactions and reactivity with external dyes—often drugs or pollutants—that can (photo)sensitize the bio-macromolecule leading to eventual chemical modifications, thus damages. In this review, we will focus on the theoretical simulation of electronic spectroscopies of bio-macromolecules, considering their secondary structure and including their interaction with different kind of (photo)sensitizers. Namely, absorption, emission and electronic circular dichroism (CD) spectra are calculated and compared with the available experimental data. Non-linear properties will be also taken into account by two-photon absorption, a highly promising technique (i) to enhance absorption in the red and infra-red windows and (ii) to enhance spatial resolution. Methodologically, the implications of using implicit and explicit solvent, coupled to quantum and thermal samplings of the phase space, will be addressed. Especially, hybrid quantum mechanics/molecular mechanics (QM/MM) methods are explored for a comparison with solely QM methods, in order to address the necessity to consider an accurate description of environmental effects on spectroscopic properties of biological systems.

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“…This technique is based on parametric down conversions occurring into a birefringent nonlinear crystal. These optical parametric oscillators or amplifiers (OPO or OPA) convert the excitation wavelengths in longer wavelengths [95]. They are pumped by a Ti: Sa source, extending the range of excitation wavelengths available between 0.5 µm up to about 2.5 µm [96][97][98].…”

Section: Laser Technology Usually Implemented On Commercial Multiphotmentioning

confidence: 99%

A review of biomedical multiphoton microscopy and its laser sources

Lefort¹

2017

J. Phys. D: Appl. Phys.

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Multiphoton microscopy (MPM) has been the subject of major development efforts for about 25 years for imaging biological specimens at micron scale and presented as an elegant alternative to classical fluorescence methods such as confocal microscopy. In this topical review, the main interests and technical requirements of MPM are addressed with a focus on the crucial role of excitation source for optimization of multiphoton processes. Then, an overview of the different sources successfully demonstrated in literature for MPM is presented, and their physical parameters are inventoried. A classification of these sources in function with their ability to optimize multiphoton processes is proposed, following a protocol found in literature. Starting from these considerations, a suggestion of a possible identikit of the ideal laser source for MPM concludes this topical review.

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When multiphoton microscopy sees near infrared

Cited by 17 publications

References 56 publications

Developmental Alterations in Heart Biomechanics and Skeletal Muscle Function in Desmin Mutants Suggest an Early Pathological Root for Desminopathies

Developmental Alterations in Heart Biomechanics and Skeletal Muscle Function in Desmin Mutants Suggest an Early Pathological Root for Desminopathies

Steady-State Linear and Non-linear Optical Spectroscopy of Organic Chromophores and Bio-macromolecules

A review of biomedical multiphoton microscopy and its laser sources

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