Whey as a renewable source for lipid production by <i>Rhodococcus</i>  strains: Physiology and genomics of lactose and galactose utilization

Herrero, O. Marisa; Alvarez, Héctor M.

doi:10.1002/ejlt.201500080

Cited by 17 publications

(8 citation statements)

References 39 publications

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“…Marcia and Alvarez investigated the production of microbial oil and biomass. Five strains of Rhodococcus were cultured on whey, and the results showed that Rhodococcus opacus produced more than 45% lipid and the remaining bacteria produced less than 5% lipid [41]. The results of the Marcia and Alvarez study indicate that all Rhodococcus bacteria are not capable of using whey for lipid production, but in the present study, the native isolate of Kocuria Y205 could produce the highest amount of lipid production from the whey substrate, indicating bacterial adaptation for lipid production from whey.…”

Section: Discussionmentioning

confidence: 45%

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Single-cell Oil Production Using Low-Cost Carbon Sources by Newly Isolated Kocuria Y205

2021

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Background & Aims of the Study: This study aimed to investigate, for the first time, the ability of single-cell oil production from low-cost carbon sources using Kocuria Y205 native Iranian bacterial isolates from the soil.Materials and Methods: Whey and lignocellulose compounds were used as carbon sources and yeast extract as a nitrogen source. Also, the isolated cultivation was done on Mineral Salts Medium (MSM) culture media. Molecular analysis based on 16s rRNA gene sequence was performed to identify isolated. Fourier-Transform Infrared Spectroscopy (FTIR) analysis was used to confirm the presence of carbon groups. GC analysis was also used to identify the fatty acids Sudan black. Finally, a Transmission Electron Microscopy (TEM) electron microscopy image was taken to view the stored lipid granule. Results:The highest rate of lipid production among all carbon sources in different periods of cultivation by whey was 24.57% after 48 hours, and also, the highest rate of lipid production for lignocellulose compounds was 15.29% after 48 hours. Conclusion:This study shows that the newly isolated Kocuria Y 205 has excellent ability to use whey and lignocellulose compounds as waste containing carbon resources for the growth, production, and storage of microbial oil. It can be used in industrial applications, too.

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Section: Discussionmentioning

confidence: 45%

“…A temperature program was used to efficiently separate the methyl esters (90°C for 5 min, temperature increase of 6°C/min, 220°C for 10 min). For quantitative analysis, tridecanoic acid was used as an internal standard [41]. GC analysis was performed on lignocellulose carbon source samples.…”

Section: Gas Chromatography (Gc) Analysismentioning

confidence: 99%

Single-cell Oil Production Using Low-Cost Carbon Sources by Newly Isolated Kocuria Y205

2021

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“…Very low growth with final biomasses of under 0.5 g L −1 were determined for cultures with galactose, lactose, and maltose. The inability to grow on galactose and lactose was reported in other Rhodococcus strains, such as R. jostii , R. erythropolis , R. fascians , and R. equi [ 50 ]. The only strain capable of growth on these carbon sources was R. opacus [ 50 ], which is also reported to grow on maltose [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Rhodococcus erythropolis JCM3201T Nutrient Media to Improve Biomass, Lipid, and Carotenoid Yield Using Response Surface Methodology

Engelhart-Straub,

Haack,

Awad

et al. 2023

Microorganisms

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The oleaginous bacterium Rhodococcus erythropolis JCM3201T offers various unique enzyme capabilities, and it is a potential producer of industrially relevant compounds, such as triacylglycerol and carotenoids. To develop this strain into an efficient production platform, the characterization of the strain’s nutritional requirement is necessary. In this work, we investigate its substrate adaptability. Therefore, the strain was cultivated using nine nitrogen and eight carbon sources at a carbon (16 g L−1) and nitrogen (0.16 g L−1) weight ratio of 100:1. The highest biomass accumulation (3.1 ± 0.14 g L−1) was achieved using glucose and ammonium acetate. The highest lipid yield (156.7 ± 23.0 mg g−1DCW) was achieved using glucose and yeast extract after 192 h. In order to enhance the dependent variables: biomass, lipid and carotenoid accumulation after 192 h, for the first time, a central composite design was employed to determine optimal nitrogen and carbon concentrations. Nine different concentrations were tested. The center point was tested in five biological replicates, while all other concentrations were tested in duplicates. While the highest biomass (8.00 ± 0.27 g L−1) was reached at C:N of 18.87 (11 g L−1 carbon, 0.583 g L−1 nitrogen), the highest lipid yield (100.5 ± 4.3 mg g−1DCW) was determined using a medium with 11 g L−1 of carbon and only 0.017 g L−1 of nitrogen. The highest carotenoid yield (0.021 ± 0.001 Abs454nm mg−1DCW) was achieved at a C:N of 12 (6 g L−1 carbon, 0.5 g L−1 nitrogen). The presented results provide new insights into the physiology of R. erythropolis under variable nutritional states, enabling the selection of an optimized media composition for the production of valuable oleochemicals or pigments, such as rare odd-chain fatty acids and monocyclic carotenoids.

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“…Hence, cheese whey represents a potential growth medium in biotechnological processes due to its nutritional profile [48]. Several researchers tried to optimize the bioprocess for producing microbial oil from cheese whey or its permeate [48,49,50,51,52,53,54]. Table 1 shows the yield of lipids produced by various microorganisms with cheese whey as feedstock.…”

Section: Bioethanol Several Countries Worldwide Includingmentioning

confidence: 99%

Emerging Trends and Advancements in the Processing of Dairy Whey for Sustainable Biorefining

Goyal

Dhyani

Chandra

et al. 2023

Journal of Food Processing and Preservation

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Increased milk production has boosted the market of milk-driven products, and as a result, the by-product production has also increased, which is a challenge to dispose of. Whey, a cheese by-product, is also increasing yearly, and its disposal in water bodies is responsible for water pollution and thus is an issue for the dairy sector. In this context, extensive research has been going on to valorize this by-product and create alternative ways to remove the organic load in whey rather than disposing of it. Recently, exciting developments have been made to convert whey into value-added commodities such as biofuels (bioethanol, biodiesel, and biohydrogen), bioplastics, bacterial cellulose, food colors and flavors, bioprotective solutions, bioactive peptides, and single-cell proteins. In this review, we aim to comprehend the recent developments and challenges in producing a whole range of value-added ingredients with whey as feedstock through microbial fermentation. Particular focus was paid to the potential of novel genetically engineered or adapted microbial strains to valorize bovine whey economically and sustainably.

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Whey as a renewable source for lipid production by Rhodococcus strains: Physiology and genomics of lactose and galactose utilization

Cited by 17 publications

References 39 publications

Single-cell Oil Production Using Low-Cost Carbon Sources by Newly Isolated Kocuria Y205

Single-cell Oil Production Using Low-Cost Carbon Sources by Newly Isolated Kocuria Y205

Optimization of Rhodococcus erythropolis JCM3201T Nutrient Media to Improve Biomass, Lipid, and Carotenoid Yield Using Response Surface Methodology

Emerging Trends and Advancements in the Processing of Dairy Whey for Sustainable Biorefining

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