Whey protein concentrate supplementation protects rat brain against aging-induced oxidative stress and neurodegeneration

Garg, Geetika; Singh, Sandeep; Singh, Abhishek Kumar; Rizvi, Syed Ibrahim

doi:10.1139/apnm-2017-0578

Cited by 24 publications

(20 citation statements)

References 55 publications

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“…The present study was designed to test the hypothesis that WPC supplementation can improve biomolecular damage, and restore the activity of membrane transporters during aging in rats. The present study is also important in view of our recent report of antiaging and neuroprotective effect of WPC in rat brain (Garg, Singh, Singh, & Rizvi, ).…”

Section: Introductionmentioning

confidence: 64%

Whey protein concentrate supplementation protects erythrocyte membrane from aging‐induced alterations in rats

Garg

Singh

et al. 2018

J Food Biochem

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Whey protein concentrate (WPC), a protein complex derived from milk, exerts a number of health benefits. The present study deals with the beneficial effects of WPC on erythrocyte membrane in rats during aging. Male Wistar rats of two different age groups: young (4 months) and old (24 months) were supplemented with WPC (300 mg/kg b.w. orally) for 28 days. The effect of WPC on age‐dependent activities of membrane transporters: Na+/K+‐ATPase, Ca2+ATPase (PMCA), and Na+/H+ Exchanger, and also on markers of oxidative stress: protein carbonyl, lipid hydroperoxides, sialic acid, and total thiol was determined. WPC supplementation ameliorated the age‐dependent alteration in the activity of membrane‐bound transporters, and biomarkers of oxidative stress in old‐aged rats. Thus, WPC could be a useful dietary regimen and may augment the ability of cells to fight damage against age‐dependent structural and functional alterations. Practical applications Anti‐aging strategies focused on nutritional modulation of health span provide an exciting area of research. Whey protein, a vital constituent of milk is rich in sulfur‐containing amino acids and has been widely accepted as an effective and safe cysteine donor for glutathione replenishment. It is acknowledged that biomarkers of oxidative stress provide a useful indicator for assessing age‐dependent alterations. Nutritional supplements which augment redox balance provide an effective intervention strategy for age‐dependent oxidative damage. In this study, we provide experimental evidence of WPC‐induced protective effect on biomarkers of aging in rats. Based on the studies on rats, we hypothesize that similar effect may be obtained in humans. Thus, WPC could be explored as a functional food to boost the cellular antioxidant levels and improve health span in humans.

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Section: Introductionmentioning

confidence: 64%

Whey protein concentrate supplementation protects erythrocyte membrane from aging‐induced alterations in rats

Garg

Singh

et al. 2018

J Food Biochem

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“…Ebaid, Salem, Sayed, and Metwalli () stated that the administration of 0.1 g/kg of body weight of whey proteins to rats lowered several oxidative stress indicators such as MDA and NO. Also, Garg, Singh, Singh, and Rizvi () reported that WPC‐supplemented rats exhibited significant reduction in oxidative stress biomarkers levels.…”

Section: Discussionmentioning

confidence: 97%

Effects of dietary probioticLactobacillus plantarumand whey protein concentrate on the productive parameters, immunity response and susceptibility of Nile tilapia,Oreochromis niloticus(L.), toAeromonas sobriainfection

AbouElatta¹,

Abdel‐Tawwab

Abdel‐Razek³

et al. 2019

Aquacult Nutr

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Immunostimulatory feed supplements have an increasingly interest in aquaculture management. Generally, an individual supplement was used in fish diets but it is expected that the use of multi-supplements may show synergistic enhancements in fish performance, health, and immunity. Therefore, the present investigation was carried out to evaluate the use of dietary probiotic Lactobacillus plantarum and whey protein concentrate (WPC) in practical diets for Nile tilapia, Oreochromis niloticus. Hence, probiotic L. plantarum, WPC and their mixture were incorporated into a basal fish diet (300 g/kg crude protein) as follows: T1 = a basal control diet, T2 = a basal diet containing L. plantarum, T3 = a basal diet containing 1.0 g WCP/kg diet and T4, T5 or T6 = basal diets containing probiotic L. plantarum + 1.0, 2.0 or 3.0 g WCP/kg diet, respectively. Fish (15.2 ± 0.6 g) were fed on one of the tested diets up to apparent satiation twice a day for 60 days. After that, fish were intraperitoneally injected with pathogenic bacteria Aeromonas sobria and fish mortality was observed for 10 days postchallenge. Fish growth and feed intake were significantly improved by dietary probiotic L. plantarum (T2) and/or WPC (T3) over the control group (T1), and highest fish performance was observed in T5-T6 fish groups. Similarly, highest values of haematocrit, glucose, total proteins, albumin, and globulin were significantly observed in T5-T6 fish groups. Likewise, fish fed dietary probiotic L. plantarum (T2), WPC (T3), and their mixture (T4-T6) showed antioxidants and immune-stimulating activities better than the control group. Fish fed the control diet were more susceptible to A. sobria infection showing highest fish mortality (75.0%). Meanwhile, dietary probiotic L. plantarum (T2), WPC (T3), and their mixture (T4-T6) enhanced significantly the fish resistance to A. sobria infection resulting in maximum values of relative percent of fish survival (73.3%-80.0%) in T5-T6 groups. The present investigation recommended the use of probiotic L. plantarum with 2.0 g WPC/kg diet to improve the growth, antioxidant, immunity responses and tolerance of Nile tilapia to A. sobria infection. Fresh whey was collected from the nearby village of the Central Laboratory for Aquaculture Research (CLAR), Abbassa, Abo-Hammad, Egypt, and transferred immediately to the Microbiology Lab, CLAR. All samples were analysed on the same day of sampling and kept at refrigerated (2-8°C) condition until doing the microbiological analysis. A 10 ml of fresh liquid whey was homogenized with 90 ml of 0.85% (w/v) sterile sodium chloride solution to make serial dilutions up to 10 −6 for each sample. A 1.0 ml sample from each of the corresponding dilutions (10 −5 and 10 −6 ) were inoculated into De Man, Rogosa and Sharpe agar (MRS agar; Merck, Germany) for lactobacilli isolation (De Man et al., 1960) and incubated at 30°C for 24-48 hr. After incubation, plates with 30-300 colonies were enumerated and the calculated results were expressed as colonyforming unit (CFU) per ml by mul...

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“…28 Whey protein was also effective in reducing ROS and RNS production in aged rats and CCL 4 -induced rat hepatotoxicity through its metal-chelating activity. 15,30 In addition, WPC decreased the hepatic lipid peroxidation and DNA damage in rats treated with aflatoxin-B1, a hepatocarcinogenic mycotoxin. 31 The improvement of liver nonenzymic antioxidants shown in the current study in the DEN-treated mice by WPC may be due to its sulfur amino acids that enhanced the production of GSH.…”

Section: Discussionmentioning

confidence: 99%

“…They prevented free radicals from damaging cellular components (such as DNA, proteins, and lipids) by directly scavenging the free radicals and/or indirectly inhibiting the processes that produced free radicals . Whey protein was also effective in reducing ROS and RNS production in aged rats and CCL 4 ‐induced rat hepatotoxicity through its metal‐chelating activity . In addition, WPC decreased the hepatic lipid peroxidation and DNA damage in rats treated with aflatoxin‐B1, a hepatocarcinogenic mycotoxin .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, hepatocyte GSH protected liver from TNF-α-induced apoptosis 36,38. Other studies found that WPC and lactoferrin protected from aging-induced neurodegeneration and inhibited the growth of lung cancer, respectively, by suppressing the release of inflammatory mediators including TNF-α 14,30. Also, WPC decreased significantly the activated caspase-3 as a result of increased GSH in the rat model of mammary tumors 12.…”

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confidence: 94%

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Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice

Mohammed

Ramadan

Zoheiry

et al. 2019

Environmental Toxicology

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Hepatocellular carcinoma is considered one of the most prevalent and lethal malignancies worldwide. Chemotherapy with cytotoxic agents showed a low response rate with possible toxic effects. Recently, some emphases have been placed on the anticancer properties of bovine whey protein and its components, especially lactoferrin. The present study aimed to evaluate and compare the antihepatocarcinogenic activity of bovine whey protein concentrate (WPC, 300 and 600 mg/kg body weight) and lactoferrin (30 and 60 mg/kg body weight), orally and daily for 14 weeks, in the mice model of diethylnitrosamine (DEN)‐induced hepatocarcinogenesis. The results showed that both WPC and lactoferrin (in a dose‐dependent manner) alleviated significantly (P < .001) the elevation in serum markers of liver carcinoma and inflammation in the DEN‐treated mice. Also, they exhibited a great amelioration in the livers' histological structure of the DEN‐treated mice by 37.0% to 66.7%. In addition, they decreased significantly (P < .001) the hepatic DNA fragmentation in the DEN‐treated mice by 23.1% to 32.7%. Only, the high doses of WPC and lactoferrin completely modulated the decrease in the activity of liver enzymic antioxidant defense system (catalase, glutathione peroxidase, and superoxide dismutase) and improved significantly (P < .01‐.001) the concentration of hepatic reduced glutathione of the DEN‐treated mice. Moreover, the high doses of WPC and lactoferrin reduced significantly (P < .05‐.001) the elevation in the concentrations of hepatic active caspases 3, 8, and 9 of the DEN‐treated mice. In conclusion, both WPC and lactoferrin were effective in inhibiting the hepatocarcinogenic activity of DEN in mice model through their ability to alleviate the hepatic inflammation and apoptosis.

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Whey protein concentrate supplementation protects rat brain against aging-induced oxidative stress and neurodegeneration

Cited by 24 publications

References 55 publications

Whey protein concentrate supplementation protects erythrocyte membrane from aging‐induced alterations in rats

Whey protein concentrate supplementation protects erythrocyte membrane from aging‐induced alterations in rats

Effects of dietary probioticLactobacillus plantarumand whey protein concentrate on the productive parameters, immunity response and susceptibility of Nile tilapia,Oreochromis niloticus(L.), toAeromonas sobriainfection

Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice

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