“…However, contrary to these findings, the membrane resistance of the 500 kDa membrane only increased until cycle 1 and reached a constant value of 3.47 Â 10 11 m À1 for successive filtration cycles (n ¼ 2e4). These results are in accordance with previous studies, where an increase in membrane resistance was reported after the first filtration cycle until it reached a stable state after multiple fouling and cleaning cycles (Blanpain-Avet et al, 2004;Mourouzidis-Mourouzis & Karabelas, 2008;Rezaei, Ashtiani, & Fouladitajar, 2011). At the beginning of a series of filtration cycles, an irreversible fouling occurred during filtration, due to deposition of protein particles and aggregates on or within the membrane, which could not be removed during cleaning process and backwashing.…”