White Environment Can Be Used as an Aversive Stimulus in Zebrafish Inhibitory Avoidance Learning

Santos, Bruno Rodrigues dos; Santos, Raissa Cruz dos; Dias, Cláudio Alberto Gellis de Mattos; Maximino, Caio; Gouvêia, Amauri

doi:10.1089/zeb.2019.1743

Cited by 7 publications

(4 citation statements)

References 41 publications

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“…This avoidance behavior can denote learning because re-exposure decreases the number of entries to environments considered dangerous. This phenomenon is observed in inhibitory avoidance tests with fishes (Santos et al, 2019).…”

Section: Discussionmentioning

confidence: 73%

Effects of re-exposure to plus maze with ramp in guppies: Habituation and sexual differences.

Walsh-Monteiro¹,

Carvalho²,

Carvalho³

et al. 2021

Psychology & Neuroscience

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One of the tools to study anxiety is behavioral animal models. Traditional models developed in rodents can be adapted to fishes. The Plus Maze with Ramp (PMR) is a fishlike-anxiety model based on the Elevated Plus Maze, a canonical model of the rodentlike-anxiety model. Literature suggests that the Elevated Plus Maze presents sex and behavior differences of rodents between the first and the other expositions to the apparatus, an effect known as "one-trial tolerance." The present study objective was to verify the effects of guppies sex and re-exposure over the exploration parameters of PMR. To do this, both sex adult groups performed five sessions (5 min) in the PMR with different intervals inter-sessions (30 min, 60 min, 120 min, 24 hr). Our data show that the PMR environment produces an avoiding behavior from the arms with ramp; the reexposure to the apparatus cause habituation to the environment, with possible tolerance, but not one-trial tolerance, with sex-dependent related effects. The data indicate the possibility and validity of PMR use as a like-anxiety model in this species. Public Significant StatementGuppies exhibit similar anxiety behavior to zebrafishes when exposed to PMR. Reexposure to PMR causes habituation in guppies, like in many vertebrates. There is sexual dimorphism in guppies behavior exposed to PMR with sensibilization only in males.

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Section: Discussionmentioning

confidence: 73%

Effects of re-exposure to plus maze with ramp in guppies: Habituation and sexual differences.

Walsh-Monteiro¹,

Carvalho²,

Carvalho³

et al. 2021

Psychology & Neuroscience

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“…Interestingly, in experiments conducted by Santos et al (2019), treatment with uoxetine in animals did not prevent the acquisition of inhibitory avoidance.…”

Section: Discussionmentioning

confidence: 94%

Tryptophan supplementation reduces body weight but does not reduce anxiety-like behavior in zebrafish

de Sousa,

Buenhombre,

Daza-Cardona

et al. 2024

Preprint

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Under certain circumstances, some nutrients can affect the synthesis of central neurotransmitters and potentially behavior. The aim of this study was to characterize the influence of tryptophan supplementation on potential anxiety-like behaviors in zebrafish (Danio rerio), as well as to assess the suitability of a new standardized diet formulation for the better maintenance and performance of this species. To achieve this, the proportion of the amino acid tryptophan supplementation was experimentally modified at 5%, 50%, and 100% levels, individually, in the zebrafish diet. The investigation was conducted through the Y-maze test, light-dark preference test, and inhibitory avoidance test. The results indicated that tryptophan supplementation did not lead to the expected reduction in anxiety-like effects in the Y-maze and light-dark tests. However, a significant difference in avoidance times was observed in the inhibitory avoidance test between the groups with 5% and 50% supplemented diets, but a significant difference in avoidance times was observed in the inhibitory avoidance test between the groups with 5% and 50% supplemented diets. Changing the diet did not alter the animal's learning or natural anxiety. However, in most animals, tryptophan supplementation led to a decrease in body mass.

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“…All animals spent more time in the black than in the white side, as expected for a control animal. This can be interpreted as an avoidance indicator [ 49 , 50 ] rather than anxiety, since these animals naturally prefer darker backgrounds that allow them to be safely hidden. Manuel, et al [ 51 ] described that enriched-raised zebrafish were less anxious in this behavioral test than the animals raised in a barren environment.…”

Section: Discussionmentioning

confidence: 99%

Housing Conditions Affect Adult Zebrafish (Danio rerio) Behavior but Not Their Physiological Status

Jorge

Félix

Costas

et al. 2023

Animals

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Zebrafish is a valuable model for neuroscience research, but the housing conditions to which it is exposed daily may be impairing its welfare status. The use of environmental enrichment and the refinement of methodology for cortisol measurement could reduce stress, improving its welfare and its suitability as an animal model used in stress research. Thus, this study aimed to evaluate (I) the influence of different housing conditions on zebrafish physiology and behavior, and (II) skin mucus potential for cortisol measurement in adult zebrafish. For this, AB zebrafish were raised under barren or enriched (PVC pipes and gravel image) environmental conditions. After 6 months, their behavior was assessed by different behavioral paradigms (shoaling, white-black box test, and novel tank). The physiological response was also evaluated through cortisol levels (whole-body homogenates and skin mucus) and brain oxidative stress markers. The results revealed that enriched-housed fish had an increased nearest neighbors’ distance and reduced activity. However, no effect on body length or stress biomarkers was observed; whole-body and skin mucus cortisol levels had the same profile between groups. In conclusion, this study highlights the skin mucus potential as a matrix for cortisol quantification, and how housing conditions could influence the data in future studies.

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White Environment Can Be Used as an Aversive Stimulus in Zebrafish Inhibitory Avoidance Learning

Cited by 7 publications

References 41 publications

Effects of re-exposure to plus maze with ramp in guppies: Habituation and sexual differences.

Effects of re-exposure to plus maze with ramp in guppies: Habituation and sexual differences.

Tryptophan supplementation reduces body weight but does not reduce anxiety-like behavior in zebrafish

Housing Conditions Affect Adult Zebrafish (Danio rerio) Behavior but Not Their Physiological Status

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