White-fruited strawberry genotypes are not per se hypoallergenic

Franz-Oberdorf, Katrin; Eberlein, Bernadette; Edelmann, Kathrin; Bleicher, Philip; Kurze, Elisabeth; Helm, Dominic; Olbricht, Klaus; Darsow, Ulf; Ring, Johannes; Schwab, Wilfried

doi:10.1016/j.foodres.2017.07.076

Cited by 13 publications

(13 citation statements)

References 64 publications

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“…From these results, we concluded that fruit color is not indicative of allergenicity related to Fra a 1.01. This finding was consistent with those of two previous studies on European varieties harvested in spring (Franz-Oberdorf et al, 2017;Kurze et al, 2018). One of the major reasons for no interaction between Fra a 1 expression and fruit color is the genetic variation in the flavonoid biosynthesis pathway in each white cultivar, that is, different mutations in an enzyme like phenylalanine ammonia-lyase (PAL) and/or dihydrokaempferol 4reductase (DFR), a transcription factor like MYB, or a binding protein to metabolic intermediates like Fra a 1.…”

Section: Rough Screening Of Cultivarssupporting

confidence: 94%

“…Transient RNA interference of Fra a 1 reduced the red color of transgenic fruit (Muñoz et al, 2010), and the Fra a 1 protein has been shown to bind to specific flavonoids (Casañal et al, 2013). However, several studies found no relationship between Fra a 1 expression and fruit color (Franz-Oberdorf et al, 2017;Kaiser et al, 2016). To clarify this contradiction, it is essential to analyze Fra a 1 expression in white-fruited genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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Strawberry (Fragaria × ananassa) contains a major allergen, Fra a 1, which causes oral allergic syndrome. Fra a 1 is a PR-10 homolog that is regulated by environmental conditions. The allergenicity of fruit caused by Fra a 1 may depend on the genotype or growing conditions. We analyzed the Fra a 1.01 transcript levels and Fra a 1.01 protein levels in strawberry fruits of several genotypes across all seasons. In the preliminary rough screening, we selected the line WH1 bearing white fruit and the red-fruited cultivar 'Akihime'. Under the same environmental conditions, there was no significant difference in Fra a 1.01 levels between the two cultivars over several months, suggesting that receptacle color was not indicative of allergenicity caused by Fra a 1.01. Fruits cultivated under the same environmental conditions should be used for comparisons of the allergenicity among genotypes. Both 'Akihime' and WH1 accumulated significantly higher levels of Fra a 1.01 protein in winter than in spring. We investigated the effects of irradiation and low temperature as environmental factors controlling the accumulation of Fra a 1.01 in winter. A shading treatment on fruit did not significantly affect Fra a 1.01 protein accumulation in strawberry fruits. Regarding variations over time, the Fra a 1.01 protein content was higher in fruits harvested at midnight in January than in those harvested at other times and in other months. These findings suggested that the Fra a 1.01 protein accumulates in response to environmental factors such as cold stress.

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Section: Rough Screening Of Cultivarssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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“…White strawberries-colorless strawberry mutants known to be tolerated by individuals affected by allergies-were found to be virtually free from the strawberry allergen [38]. It seems that the white fruits were more easily tolerated by volunteers suffering from fruit allergies than normal fruits [39]. Moreover, the level of Bet v1 in white strawberry mutants was almost zero.…”

Section: Discussionmentioning

confidence: 99%

The Interaction between Antioxidants Content and Allergenic Potency of Different Raspberry Cultivars

et al. 2020

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Food allergies are a very serious problem among consumers. The most common food allergies involve animal products, but they can also involve fruits such as berries. We aimed to determine whether organic farming is useful for the production of high-quality and safe fruits. Three varieties of raspberries ‘Laszka’, ‘Glen Ample’ and ‘Polka’ from organic and conventional production (neighboring farms) were collected over the two years of the experiment. Quantitative and qualitative analysis of phenolic compounds was carried out, and the content of Bet v1 and profilin was determined. The organic raspberries contained a lower level of phenolic compounds, especially anthocyanins. Conventional fruits were characterized by a higher allergenic potency than organic ones. We found a strong link between their anthocyanin content and the allergy status of conventional raspberry fruits. Therefore, organically produced raspberries are safer for consumers.

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“…The genus Fragaria comprises numerous species with variations in fruit size and color including red, white and yellow fruits, content of aromatic compounds and genetic diversity with ploidy levels ranging from diploid to decaploid [ 12 ]. The octoploid (2 n = 8 x = 56) garden strawberry F .…”

Section: Introductionmentioning

confidence: 99%

Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

et al. 2018

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Birch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries (Fragaria × ananassa). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0.67 to 3.97 µg/g fresh weight. Mature fruits of red-, white- and yellow-fruited strawberry cultivars showed similar Fra a 1 concentrations. Compared to fresh strawberries, oven and solar-dried fruits contained slightly lower levels due to thermal treatment during processing. SDS-PAGE and Western blot analysis demonstrated degradation of recombinant Fra a 1.02 after prolonged (>10 min) thermal treatment at 99 °C. In conclusion, the genotype strongly determined the Fra a 1 quantity in strawberries and the color of the mature fruits does not relate to the amount of the PR10-protein. Cultivation conditions (organic and conventional farming) do not affect the Fra a 1 level, and seasonal effects were minor.

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White-fruited strawberry genotypes are not per se hypoallergenic

Cited by 13 publications

References 64 publications

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

The Interaction between Antioxidants Content and Allergenic Potency of Different Raspberry Cultivars

Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

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