White matter tract conductivity is resistant to wide variations in paranodal structure and myelin thickness accompanying the loss of Tyro3: an experimental and simulated analysis

Blades, Farrah; Chambers, Jordan D; Aumann, Tim D.; Nguyen, Christine T. O.; Wong, Vickie; Aprico, Andrea; Nwoke, Eze C; Bui, Bang V.; Grayden, David B.; Kilpatrick, Trevor J.; Binder, Michele D.

doi:10.1007/s00429-022-02489-8

Cited by 3 publications

(2 citation statements)

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“…Experiments using the Tyro3 −/− were approved by the Animal Ethics Committee at the Florey Institute of Neuroscience and Mental Health and conducted in accordance with the National Health and Medical Research Council (Australia) Guidelines. Tyro3 knockout ( Tyro3 -/- ) mice were fully backcrossed onto the C57BL/6J background and maintained under specific-pathogen-free conditions throughout breeding and experimentation, as described previously (24). The original Tyro3 -/- line was kindly provided by Prof. Greg Lemke from the Salk Institute of Biological Studies, La Jolla, CA (20) and currently maintained by M. Binder at the Florey Institute of Neuroscience and Mental Health.…”

Section: Methodsmentioning

confidence: 99%

“…During the early stages of mouse gonad differentiation, TYRO3 appears to be expressed in males only. It is expressed in Sertoli cells of the testis from E11.5 onwards, whereas no TYRO3 signal was observed in ovaries until E17.5 (23). In mouse embryos, Axl and Mertk are expressed in Sertoli cells and Leydig cells in male gonads, and granulosa cells and stromal cells in female gonads from E11.5 to E13.5 (16).…”

Section: Manuscript Introductionmentioning

confidence: 99%

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Roles of TYRO3 Family Receptors in Germ Cell Development During Mouse Testis Formation

Ming,

Bagheri-Fam,

Frost

et al. 2024

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Objective: To investigate the role of a potential SOX9 target gene, Tyro3, along with its family members, Axl and Mertk (TAM family) in mouse testis development. Design: Experimental laboratory study. Setting: Research institute units. Subject(s): Embryonic day (E)11.5 Swiss mouse gonads for ex vivo gonad culture; Tyro3 knockout mouse embryos. Intervention(s): E11.5 Swiss mouse gonads were cultured in hanging droplets of 30 μL DMEM medium supplemented with 10% FBS and 1% antibiotic-antimycotic. A pair of gonads were treated with 20 μM of BMS-777607 or 30 μM of LDC1267 and an equivalent volume of the vehicle control DMSO. Main Outcome Measure(s): Immunofluorescence to measure morphological changes of ex vivo cultured gonads and in vivo Tyro3 mouse testes; qRT-PCR to measure gene expressions. Result(s): Inhibition of the TAM family in E11.5 ex vivo cultured male mouse gonads led to reduced germ cell numbers caused by reduced proliferation and increased apoptosis of the germ cells. Tyro3 knockout mice exhibited reduced expression levels of the germ cell genes Ddx4, Dazl and Pou5f1 and increased expression levels of the Sertoli cell genes Sox9 and Amh at E12.5. However, by E14.5, the expression of Ddx4, Dazl, Sox9 and Amh had returned to normal levels in Tyro3 knockout testes. Tyro3 knockout testes displayed normal morphology and structures during fetal testis development. Conclusion(s): TAM family members have redundant roles in regulating germ cell development during early testis development.

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Section: Methodsmentioning

confidence: 99%

Section: Manuscript Introductionmentioning

confidence: 99%