WHITE PANICLE1, a Val-tRNA Synthetase Regulating Chloroplast Ribosome Biogenesis in Rice, Is Essential for Early Chloroplast Development

Wang, Yunlong; Wang, Chunming; Zheng, Ming; Lyu, Jia; Xu, Yang; Li, Xiaohui; Niu, Mei; Long, Wuhua; Wang, Di; Wang, Haiyang; Terzaghi, William; Wang, Yihua; Wan, Jianmin

doi:10.1104/pp.15.01949

Cited by 76 publications

(55 citation statements)

References 50 publications

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“…qRT-PCR results indicate that the expression levels of plastid-encoded genes decreased and nuclear-encoded genes increased in wfsl1 . These are very likely previously reported mutant wp1 9 . The wp1 mutant decreased plastid-encoded proteins and defective in chloroplast developmental.…”

Section: Discussionsupporting

confidence: 86%

Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice

Wang

et al. 2017

Sci Rep

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Plant leaves are a crucial organ associated closely with chloroplast development, photosynthesis rate and crop productivity. In this study, a white fine stripe leaf 1 (wfsl1) mutant was isolated and characterized from the japonica rice Zhonghua11 (ZH11) after ethyl methanesulfonate mutagenesis. The wfsl1 displayed white fine stripe leaves since tillering stage and abnormal chloroplast structure. Map-based cloning and Bioinformatic analysis indicated that WFSL1 on chromosome 1 contains an “A” to “T” substitution in protein coding region, and encodes a putative metal-dependent phosphohydrolase with HD domain at the N-terminus. WFSL1 was targeted to the chloroplasts and had higher expression in mature leaves and sheaths. RNA-seq analysis revealed that chloroplast development and photosynthesis genes were significantly affected in wfsl1 plants. Levels of WFSL1 and chloroplast encoded proteins were decreased in wfsl1 mutants via western blot analysis. Compared with WT, wfsl1 exhibits lower Chl content and defective in biogenesis of chloroplast ribosomes, which resulted in reduced grain yield. Taken together, our results show that WFSL1 is critical for chloroplast development, ribosome biogenesis, and light energy utilization, finally affects grain yield.

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Section: Discussionsupporting

confidence: 86%

Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice

Wang

et al. 2017

Sci Rep

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“…Genes involved in the variegation pattern formation have been cloned in monocot and dicot species (e.g. Wu et al, 1999; Chen et al, 2000; Takechi et al, 2000; Prikryl et al, 2008; Hayashi-Tsugane et al, 2014; Wang et al, 2016; Zheng et al, 2016; Guan et al, 2017; Zagari et al, 2017). Despite the advances of identifying the underlying genes, insights to the molecular mechanisms controlling the phenomenon are rare (Sakamoto, 2003; Yu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Leaf Variegation and Impaired Chloroplast Development Caused by a Truncated CCT Domain gene inalbostriansBarley

Hensel

Mascher

et al. 2019

Preprint

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Chloroplasts fuel plant development and growth by converting solar into chemical energy. They mature from proplastids through the concerted action of genes in both the organellar and the nuclear genome. Defects in such genes impair chloroplast development and may lead to pigment-deficient seedlings or seedlings with variegated leaves. Such mutants are instrumental as tools for dissecting genetic factors underlying the mechanisms involved in chloroplast biogenesis. Characterization of the green-white variegated albostrians mutant of barley has greatly broadened the field of chloroplast biology including the discovery of retrograde signaling. Here, we report the identification of the ALBOSTRIANS gene HvAST by positional cloning as well as its functional validation based on independently induced mutants by TILLING and RNA-guided Cas9 endonuclease mediated gene editing. The phenotypes of the independent HvAST mutants imply residual activity of HvAST in the original albostrians allele conferring an imperfect penetrance of the variegated phenotype even at homozygous state of the mutation. HvAST is a homolog of the Arabidopsis thaliana CCT Motif transcription factor gene AtCIA2, which was reported to be involved in the expression of nuclear genes essential for chloroplast biogenesis. Interestingly, in barley we localized HvAST to the chloroplast indicating novel without any clear evidence of nuclear localization.One-sentence summaryLeaf variegation in the barley mutant albostrians is caused by mutation of a single CCT-domain containing gene with residual activity, which is directed to the chloroplast.

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“…Some of these have been shown to mediate similar functions in chloroplasts (for review, see Bohne, 2014;Liu et al, 2015;Jeon et al, 2017;Janowski et al, 2018). Despite the dual genetic origin of plastid RPs, which necessitates spatiotemporal orchestration of RP assembly, and significant structural differences with respect to bacterial ribosomes, few chloroplast-specific ribosome biogenesis factors have been identified to date (Bohne, 2014;Wang et al, 2016;Meurer et al, 2017;Paieri et al, 2018;Pulido et al, 2018).…”

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confidence: 99%

The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis

et al. 2020

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Biogenesis of plastid ribosomes is facilitated by auxiliary factors that process and modify ribosomal RNAs (rRNAs) or are involved in ribosome assembly. In comparison with their bacterial and mitochondrial counterparts, the biogenesis of plastid ribosomes is less well understood, and few auxiliary factors have been described so far. In this study, we report the functional characterization of CONSERVED ONLY IN THE GREEN LINEAGE20 (CGL20) in Arabidopsis (Arabidopsis thaliana; AtCGL20), which is a Pro-rich, ;10-kD protein that is targeted to mitochondria and chloroplasts. In Arabidopsis, CGL20 is encoded by segmentally duplicated genes of high sequence similarity (AtCGL20A and AtCGL20B). Inactivation of these genes in the atcgl20ab mutant led to a visible virescent phenotype and growth arrest at low temperature. The chloroplast proteome, pigment composition, and photosynthetic performance were significantly affected in atcgl20ab mutants. Loss of AtCGL20 impaired plastid translation, perturbing the formation of a hidden break in the 23S rRNA and causing abnormal accumulation of 50S ribosomal subunits in the high-molecular-mass fraction of chloroplast stromal extracts. Moreover, AtCGL20A-eGFP fusion proteins comigrated with 50S ribosomal subunits in Suc density gradients, even after RNase treatment of stromal extracts. Therefore, we propose that AtCGL20 participates in the late stages of the biogenesis of 50S ribosomal subunits in plastids, a role that presumably evolved in the green lineage as a consequence of structural divergence of plastid ribosomes.

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WHITE PANICLE1, a Val-tRNA Synthetase Regulating Chloroplast Ribosome Biogenesis in Rice, Is Essential for Early Chloroplast Development

Cited by 76 publications

References 50 publications

Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice

Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice

Leaf Variegation and Impaired Chloroplast Development Caused by a Truncated CCT Domain gene inalbostriansBarley

The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis

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