White peroxidase-mimicking nanozyme˗nanocarrier of enzyme labeled antibody to enhance catalytic performance and relieve color interference of immunoassay

Li, Yuechun; Wang, Lulu; Cui, Zhaowen; Liu, Sijie; Wang, Shaochi; Ren, Jiawei; Tian, Yanli; Shu, Rui; Luo, Xing; Liao, Yangjun; Wang, Jianlong; Zhang, Daohong

doi:10.1016/j.snb.2022.131909

Cited by 18 publications

(8 citation statements)

References 44 publications

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“…Optimization of the OFO-FL Immunosensor. In order to obtain the best performance of the Nb2−5-APmediated fluorescent ELISA for AFB 1 , several factors were optimized, including the concentration of mAb 1C11 (2, 3, 4, 5 μg/mL), Nb2−5-AP (0.25, 2.5, 1, 2 μg/mL), pH value (6,7,8,9), and methanol concentration (5, 10, 20, 40%). Based on the half the maximal inhibitory concentration (IC 50 ) and FL max/min as the optimized evaluation criterion, 2 μg/mL of 1C11, 1 μg/ mL of Nb2−5-AP, pH 7, and 40% of methanol content were selected as the optimal working conditions for further analysis (Figure 4).…”

Section: Characterization Of Nb2−5-apmentioning

confidence: 99%

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Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On–Off–On Fluorescence Immunosensor for Aflatoxin in Cereals

Yu,

Wang,

Zhang

et al. 2023

J. Agric. Food Chem.

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Nanobodies (Nbs) are widely used in immunoassays with the advantages of small size and high stability. Here, the nanobody employed as the surrogate of aflatoxin antigen and the recognition mechanism of antiaflatoxin mAb with nanobody was studied by molecular modeling, which verified the feasibility of Nbs as antigen substitutes. On this basis, a nanobody-alkaline phosphatase fusion protein (Nb-AP) was constructed, and a highly sensitive "on−off−on" fluorescent immunosensor (OFO-FL immunosensor) based on the calcein/Ce 3+ system was developed for aflatoxin quantification. Briefly, calcein serves as a signal transducer, and its fluorescence can be quenched after it is bound with Ce 3+ . In the presence of Nb-AP, AP catalyzed p-nitrophenyl phosphate to generate orthophosphate, which competes in binding with Ce 3+ , leading to fluorescence recovery. The method has a linearity range of 0.005−100 ng/mL, and the IC 50 of the OFO-FL immunosensor was 0.063 ng/mL, which was 18-fold lower than that of conventional enzyme-linked immunosorbent assay. The assay was successfully applied in food samples with a recovery of 88− 121%.

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Section: Characterization Of Nb2−5-apmentioning

confidence: 99%

“…5 Thus, rapid detection methods with high sensitivity and specificity are urgently needed. Immunoassays, based on the specific binding of antigen− antibody, have the unique ability to routinely handle a large number of samples with high sensitivity, 6,7 making it a prevailing technique for detecting AFs.…”

Section: Introductionmentioning

confidence: 99%

Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On–Off–On Fluorescence Immunosensor for Aflatoxin in Cereals

Yu,

Wang,

Zhang

et al. 2023

J. Agric. Food Chem.

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“…Therefore, the utilization of nanozyme showed great potential to substitute for enzyme in the sensing of agroproducts. Taking these factors into consideration, nanozyme-based sensors with rapidity, economic, and operational features have promoted agroproduct safety in the new era . Herein, the different types of nanozyme-based sensors are comprehensively discussed, covering colorimetric, SERS, fluorescent, and others.…”

Section: Applications Of Nanozymes In Agriculturementioning

confidence: 99%

Lighting Up Agricultural Sustainability in the New Era through Nanozymology: An Overview of Classifications and Their Agricultural Applications

Cui

Zhang

et al. 2022

J. Agric. Food Chem.

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With the concept of sustainable agriculture receiving increasing attention from humankind, nanozymes, nanomaterials with enzyme-like activity but higher environmental endurance and longer-term stability than natural enzymes, have enabled agricultural technologies to be reformative, economic, and portable. Benefiting from their multiple catalytic activities and renewable nanocharacteristics, nanozymes can shine in agricultural scenarios using enzyme engineering and nanoscience, acting as sustainable toolboxes to improve agricultural production and reduce the risk to agricultural systems. Herein, we comprehensively discuss the classifications of nanozymes applied in current agriculture, including peroxidase-like, oxidase-like, catalase-like, superoxide dismutase-like, and laccase-like nanozymes, as well as their biocatalytic mechanisms. Especially, different applications of nanozymes in agriculture are deeply reviewed, covering crop protection and nutrition, agroenvironmental remediation and monitoring, and agroproduct quality monitoring. Finally, the challenges faced by nanozymes in agricultural applications are proposed, and we expect that our review can further enhance agricultural sustainability through nanozymology.

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“…Enzyme‐linked immunosorbent assays (ELISA), based on specific combinations of antibodies and antigens, have been widely applied in environmental monitoring, medical diagnosis, and food safety (Li, Wang, et al., 2022). In a typical ELISA, the output signal is generated by oxidation of the substrate by horseradish peroxidase (HRP).…”

Section: Mechanisms Of Nldsmentioning

confidence: 99%

Nanozyme‐encoded luminescent detection for food safety analysis: An overview of mechanisms and recent applications

Sun²,

Huang

et al. 2022

Comp Rev Food Sci Food Safe

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With the rapid growth in global food production, delivery, and consumption, reformative food analytical techniques are required to satisfy the monitoring requirements of speed and high sensitivity. Nanozyme-encoded luminescent detections (NLDs) integrating nanozyme-based rapid detections with luminescent output signals have emerged as powerful methods for food safety monitoring, not only because of their preeminent performance in analysis, such as rapid, facile, low background signal, and ultrasensitive, but also due to their strong attractiveness for future sensing research. However, the lack of a full understanding of the fundamentals of NLDs for food safety detection technologies limits their further application. In this review, a systematic overview of the mechanisms of NLDs and their applications in the food industry is summarized, which covers the nanozyme-mimicking types and their luminescent signal generation mechanisms, as well as their applications in monitoring common foodborne contaminants. As demonstrated by previous studies, NLDs are bridging the gap to practical-oriented food analytical technologies and various opportunities to improve their food analytical performance to be considered in the future are proposed.

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White peroxidase-mimicking nanozyme˗nanocarrier of enzyme labeled antibody to enhance catalytic performance and relieve color interference of immunoassay

Cited by 18 publications

References 44 publications

Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On–Off–On Fluorescence Immunosensor for Aflatoxin in Cereals

Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On–Off–On Fluorescence Immunosensor for Aflatoxin in Cereals

Lighting Up Agricultural Sustainability in the New Era through Nanozymology: An Overview of Classifications and Their Agricultural Applications

Nanozyme‐encoded luminescent detection for food safety analysis: An overview of mechanisms and recent applications

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