White shrimp (Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae

Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein

“…And the activity of refold HdLysC fusion protein was measured by turbiditimetric assay, the results showed that the recombinant protein has the activity against both Gram-positive and Gram-negative bacteria while the insect lysozyme only lysed the Gram-positive bacteria [57]. However, the recombinant c-type lysozyme of kuruma shrimp Marsupenaeus japonicus [24], black tiger shrimp, Penaeus monodon [26] and white shrimp Litopenaeus vannamei [30], the recombinant g-type lysozyme of zhikong scallop C. farreri [15], the purified i-type lysozyme from Icelandic scallop C. islandica [34] and eastern oysters C. virginica [58] all have the activity inhibiting the growth of Gram-positive bacteria and Gram-negative bacteria. These results according with hypothesize that the marine invertebrates lysozymes have a wider range of activities than those of terrestrial invertebrates in order to cope with a greater range of bacterial strains and species in the marine environment [24].…”

“…To confirm the relative bacteriolytic activity of lysozyme, the viable counts were determined by spread plate method from the initial and final samples [29]. The bacteriolytic activity was determined as described by de-la-Re-Vega et al [30].…”

Insights into the immune transcriptome of the shrimpLitopenaeus vannamei: tissue-specific expression profiles and transcriptomic responses to immune challenge

“…Clustergrams with similar branch structures were produced regardless of the tissue dataset used as the calibrator/reference (data not shown), indicating the robustness of the association between the profiles observed in hemocytes and in gills. This may reflect the fact that circulating (HP; 1, 2, 3), muscle (4,5,6), hemocytes (hem ; 7, 8, 9), or gills (10,11,12). Clones are clustered in rows according to common patterns of expression along the 12 samples, while samples are clustered in columns according to similarities in global expression profiles.…”

“…The mRNAs for lysozyme, a homolog of tachylectin (from horseshoe crab), and antilipopolysaccharide factor were significantly upregulated in infected hepatopancreas. The antimicrobial activity of these proteins (against bacteria and fungi) has been well established (12,24,29,50), but their potential involvement in antiviral responses has remained largely unexplored. Overall, these results suggest that infection with WSSV activates responses that overlap (at least partially) with those induced by challenge with bacteria (51,52).…”

Lysozyme gene expression by hemocytes of Pacific white shrimp, Litopenaeus vannamei, after injection with Vibrio

“…Penaeid shrimp lysozymes are well characterized, hemocyte-specific proteins that have been shown to possess lytic activity against a range of Gram positive and Gram negative bacterial species, including pathogenic Vibrio spp. [23,25]. Lysozyme enzymatic activity was specific to hemocyte lysate, being absent in cell-free hemolymph, and was estimated to comprise approximately 4% of the total hemocyte protein pool in L. vannamei [24].…”