White Spot Syndrome Virus infection in Penaeus monodon is facilitated by housekeeping molecules

Abstract

White Spot Syndrome Virus (WSSV) is a major pathogen in shrimp aquaculture, and its rampant spread has resulted in great economic loss. Identification of host cellular proteins interacting with WSSV will help in unravelling the repertoire of host proteins involved in WSSV infection. In this study, we have employed one-dimensional and two-dimension virus overlay protein binding assay (VOPBA) followed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify the host proteins of Penaeus monodon that could interact with WSSV. The VOPBA results suggest that WSSV interacted with housekeeping proteins such as heat shock protein 70, ATP synthase subunit beta, phosphopyruvate hydratase, allergen Pen m 2, glyceraldehyde-3-phosphate dehydrogenase, sarcoplasmic calcium-binding protein, actin and 14-3-3-like protein. Our findings suggest that WSSV exploits an array of housekeeping proteins for its transmission and propagation in P. monodon.

A Proteomic Study of Hemocyte Proteins from Mud Crab (Scylla paramamosain) Infected with White Spot Syndrome Virus or Vibrio alginolyticus

“…Sarcoplasmic calcium-binding protein is also an important allergen in the muscle and shell of Fenneropenaeus merguiensis (24). WSSV infection in Penaeus monodon is facilitated by housekeeping molecules like sarcoplasmic calcium-binding protein 1 (25). When sarcoplasmic calcium-binding protein 1 was knocked down, WSSV infection would cause the animals to die faster and more often, but in V. alginolyticus infection this would only reduce the lifespan of infected crabs but decreased the overall mortality rates.…”

Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection

“…Intracellular Ca 2+ concentration is critical in the viral life cycle (capsid transport) and influences many host cell defense responses such as apoptosis [67,68]. The down-expression of the gene scp was already observed in post-larvae of the shrimp Penaeus modon infected with WSSV [69], and was revealed by 2-DE proteomic study in the stomachs of the shrimp Penaeus vannamei challenged with WSSV [17,70]. Interestingly, the genome of OsHV-1 contains one ORF which encodes a cellular chloride ion channel (ORF 57) [71] that would allow the virus to modulate the host Ca 2+ homeostasis.…”

Identification and genotyping of white spot syndrome virus (WSSV) isolated from Litopenaeus vannameiin the state Rio Grande do Norte

“…In addition, temperatures between 22 and 30°C increased the replication efficiency of WSSV (SUN et al, 2014) in the early stages of acute infection, which coincides with the optimum temperature range for juvenile shrimp when uninfected (RAHMAN et al, 2006). Molecular interactions between the virus and the activity of constituent genes, important for the development of Penaeus monodon, such as hsp70 and ATP synthetase, were identified; thereby, it is suggested that the WSSV benefits from the phase in which there is more development of the host (BIRADAR et al, 2013). The study of these factors becomes important to understand the cyclic episodes of infection, whether by the recurrence of the same genotype or by the introduction of new viral variants.…”