Who Could Be Blamed in the Case of Discrepant Histology and Serology Results for Helicobacter pylori Detection?

Skrebinska, Sabīne; Mégraud, Françis; Daugule, Ilva; Šantare, Daiga; Isajevs, Sergejs; Liepniece-Karele, Inta; Bogdanova, Inga; Rudzite, Dace; Vangravs, Reinis; Ķikuste, Ilze; Vanags, Aigars; Tolmanis, Ivars; Savcenko, Selga; Alix, C.; Herrero, Rolando; Park, Jin Young; Leja, Mārcis

doi:10.3390/diagnostics12010133

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…Anti- H. pylori antibodies, which reveal how the gastric mucosa reacts to an exogenous pathogen, have also been investigated as a diagnostic marker for gastric cancer. However, the value of antibodies in serum is low because they remain positive long after H. pylori has been successfully eliminated (Skrebinska et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Wang et al (2000) used transgenic mice overexpressing amidated gastrin-17 to show that this peptide and H. pylori infection had a synergic effect in the advancement of gastric adenocarcinoma (Song et al, 2003;Smith et al, 2017;Duan et al, 2022) Anti-H. pylori antibodies, which reveal how the gastric mucosa reacts to an exogenous pathogen, have also been investigated as a diagnostic marker for gastric cancer. However, the value of antibodies in serum is low because they remain positive long after H. pylori has been successfully eliminated (Skrebinska et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Assessing the utility of pepsinogens and gastrin-17 in gastric cancer detection

Gasenko¹,

Bogdanova²,

Sjomina

et al. 2023

European Journal of Cancer Prevention

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ObjectivesThe aim of the study was to determine the proportion of gastric cancer patients with decreased levels of pepsinogen and gastrin-17 in plasma, with the goal of providing indirect evidence of the sensitivity of these biomarkers when applied in a cancer screening setting. MethodsThe levels of pepsinogens I and II, gastrin-17, and Helicobacter pylori immunoglobulin antibodies in plasma samples of gastric cancer patients were evaluated using the GastroPanel test system (Biohit Oyj, Helsinki, Finland). A decreased level of the pepsinogen I/ II ratio was defined as less than three, while a decrease in gastrin-17 was defined as less than 1 pmol/L. Univariate analysis using non-parametric tests was used to investigate differences between normal and low concentrations of biomarkers. ResultsIn total, 481 plasma samples from patients (59.9% male) with a median age of 64 years (ranging from 27 to 88 years) were analyzed. Out of the 400 cases of gastric cancer (83.2% of the total), 182 were categorized as the intestinal type, 141 as the diffuse type, 60 as the mixed type, and 17 as indeterminate according to the Lauren classification system. The H. pylori immunoglobulin test was positive in 74.0% of the patients. Pepsinogen I/ II ratio was decreased in 32.4% (36.8% of the intestinal type); gastrin-17 in 12.3% (10.1% of the antral region) of all cases. ConclusionThe majority of gastric cancer patients had normal levels of pepsinogen and gastrin-17, suggesting that these biomarkers have limited application as screening tools in the Caucasian population. European

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessing the utility of pepsinogens and gastrin-17 in gastric cancer detection

Gasenko¹,

Bogdanova²,

Sjomina

et al. 2023

European Journal of Cancer Prevention

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“…Bogiel et al conducted a study on children where they saw a high concordance between the results of H. pylori detection using histopathological investigation and molecular methods [21]. Also, in the study conducted by Srebinska et al, most cases were correctly diagnosed by the real-time PCR method [22]. Some authors claim that UBT and histology, like classical methods, have similar accuracy and could be verified by the PCR method only in some instances [23,24].…”

Section: Discussionmentioning

confidence: 97%

Pattern of Primary Resistance of Helicobacter pylori to Clarithromycin among Pediatric Patients from North-Eastern Romania

Rosu,

Gimiga,

Popescu

et al. 2023

Children

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Background: Helicobacter pylori antibiotic resistance has increased worldwide and affects the effectiveness of current therapies. The recommended first-line empiric treatment should be tailored to the local clarithromycin resistance rate. This study aimed to determine the pediatric patient profile and rate of clarithromycin resistance for patients diagnosed with Helicobacter pylori by gastric biopsy. Methods: We studied 84 positive gastric samples for Helicobacter pylori. Positive results were confirmed by a rapid urease test and histopathological examination, with the type of gastritis established according to the Sydney System. Gastric biopsy samples were stored in RNA saver. Clarithromycin resistance was determined by a real-time polymerase chain reaction-based molecular assay after RNA-DNA extraction. Results: Of the 84 biopsy samples analyzed, 35 (41.6%) were resistant to clarithromycin. Clarithromycin resistance was found mainly in girls (80%) with a mean age of 15 years (range 6–17 years). The history of prior exposure to clarithromycin was 91.6%. The concordance between the histopathological examination and the PCR test was 100%. Conclusions: One in 2.4 children infected with Helicobacter pylori had a strain resistant to clarithromycin. This resistant strain may be a reason for treatment failure in Romanian children, yet this is uninvestigated. The high rate of bacterial resistance to this antibiotic among children indicates the need for susceptibility testing before therapy.

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“…Although histochemical testing is a reliable method for detecting H. pylori infection, its accuracy can be confounded by several factors, such as the distribution of H. pylori, the quality of the instrumentation, and the professional inadequacy of the pathologist. 37 Two guidelines 13,15 recommend that histological examination should be combined with special chemical staining to make a diagnosis under the guidance of an experienced pathologist. It is undeniable that endoscopy experts are equally important because they determine whether the collection of specimens is standardized and reasonable.…”

Section: Ta B L E 3 (Continued)mentioning

confidence: 99%

Evaluation of guidelines for diagnosis and treatment of Helicobacter pylori infection

Shi

Hei

et al. 2022

Helicobacter

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Background To systematically evaluate the quality of the guidelines for the diagnosis and treatment of Helicobacter pylori infection and to analyze the differences and reasons for the key recommendations in the guidelines. Methods Databases and websites were systematically searched to obtain guidelines for the diagnosis and treatment of Helicobacter pylori infection. Four independent reviewers used the Guideline Evaluation Tool (AGREE II) to evaluate the included guidelines. The intraclass correlation coefficient (ICC) and Fleiss' kappa coefficient were used to measure the consistency of evaluation guidelines between guide reviewers. Differences between guidelines and the reasons for the differences were analyzed by comparing the recommendations of different guidelines and the evidence supporting the recommendations. Results A total of 17 guidelines for Helicobacter pylori infection were included in this study. The AGREE II scores of these guidelines were low overall, with 4 of them had a score of over 60%, which indicates that the guidelines are recommended, and 13 of them having a score ranging from 30 to 60%, which indicates that the guidelines are recommended but need to be revised, while no guideline had a score of 30% or less, which indicates that they were not recommended. The analysis of these guidelines found that there were some differences in the main recommendations. Not all guidelines recommend sequential therapy as the recommended therapy. Whether bismuth quadruple therapy should be used as the recommended first‐line therapy is unclear. The antibiotic resistance rate is different in different regions. Combined with the local antibiotic sensitivity test, the eradication rate of Helicobacter pylori can be improved. Conclusion There are significant differences in the quality of Helicobacter pylori infection guidelines and the key recommendations. Improving the deficiencies of existing guidelines is an effective way to develop high‐quality guidelines and make reasonable recommendations for the treatment of Helicobacter pylori infection in the future.

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Who Could Be Blamed in the Case of Discrepant Histology and Serology Results for Helicobacter pylori Detection?

Cited by 8 publications

References 24 publications

Assessing the utility of pepsinogens and gastrin-17 in gastric cancer detection

Assessing the utility of pepsinogens and gastrin-17 in gastric cancer detection

Pattern of Primary Resistance of Helicobacter pylori to Clarithromycin among Pediatric Patients from North-Eastern Romania

Evaluation of guidelines for diagnosis and treatment of Helicobacter pylori infection

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