Whole blood chemiluminescence as a systemic inflammatory parameter in asthma

Nordman, Susann A. S.; Nyberg, Pia

doi:10.1016/0091-6749(94)90153-8

Cited by 9 publications

(6 citation statements)

References 35 publications

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“…These ROS play several roles in toxicity toward microrganisms, in generating chemotactic factors (hence recruiting additional phagocytes), and also in interacting with the signal transduction pathways. Interestingly, tbe oxidative burst is coupled to ultraweak light emission [47], a process typically involving free radical formation [48,49|.…”

Section: Phagocytosismentioning

confidence: 99%

A survey of reactive oxygen species and their role in dermatology

Maccarrone

Catani

Iraci

et al. 1997

Acad Dermatol Venereol

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General description In this paper we present a survey of the main reactive oxygen speeies (ROS), their biological sources and the array of antioxidant enzymes and antiradical molecules which conslituies ihe cellular defense against ROS toxieity. We outline also ihe fundamentals of ROS biochemistry, in relation to the role of ROS in several human pathologies. Finally, we discuss with particular emphasis the involvement of ROS in dermatological diseases, highlighting the possible therapeutic application of antioxidants in skin care.Learning objective The reader will learn what ROS are. the identity of the anti ROS defensive system in human cells and the manyfold roles of ROS in human physiopathology, and thus the potential application of antioxidants in clinical therapy. © 1997 Elsevier Science B.V.

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Section: Phagocytosismentioning

confidence: 99%

A survey of reactive oxygen species and their role in dermatology

Maccarrone

Catani

Iraci

et al. 1997

Acad Dermatol Venereol

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“…Lucigenin has a high specificity for the superoxide radical, and thus lucigenin-amplified CL reflects the activity of the NADPH oxidase complex. The luminol-amplified CL has been shown to be nearly completely dependent on MPO (4-8) and, moreover, on (26), respiratory disorders (27), diabetes (28), arthritis (29) and neoplasia (30).…”

Section: Introductionmentioning

confidence: 99%

Particle‐induced myeloperoxidase release in serially diluted whole blood quantifies the number and the phagocytic activity of blood neutrophils and opsonization capacity of plasma

Lilius

Nuutila

2006

Luminescence

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Luminol-amplified chemiluminescence (CL) from phagocytes has previously been shown to be almost completely dependent on the release of myeloperoxidase (MPO) from azurophilic granules. We measured the luminol-amplified chemiluminescence response (WBCL) by using serially diluted whole blood. In these experiments, non-opsonized and serum-opsonized zymosan (NWBCL and OWBCL, respectively) were used concurrently as phagocytosable particles. We found two whole-blood dilution ranges with clinical significance: first, <0.04% of whole blood in the reaction volume, where MPO released by the zymosan-activated leukocyte population came almost totally from neutrophils and the OWBCL response could be exploited as a measure of a neutrophil count in a given blood specimen, despite the pathophysiological state of the host. In contrast, the NWBCL response was two-fold in blood samples from bacterial infection patients compared to those of controls and patients with viral infection, suggesting the use of NWBCL for the differential diagnosis of bacterial infections from viral infections; second, 0.16-1.2% of whole blood in the reaction volume, where the opsonization capacity of plasma (OC(50)) can be determined. We also found that at whole blood content >0.04%, erythrocytes quickly start to absorb chemiluminescence light, and that at whole blood content >1.2%, plasma proteins, most probably albumin and fibrinogen, start to inhibit MPO release.

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“…40 Chemiluminescence of whole blood is suggested as one of the parameters for monitoring the in¯ammatory process in asthma. 41 In a preliminary study, two homoeopathic medicines frequently used for in¯ammation, Belladonna and Ferrum phosphoricum, at 5c and 9c dilutions, had a statistically signi®cant inhibiting effect 42 after activation of the neutrophil leucocytes, by very weak concentrations of the stimulating agent, zymosan. As the difference in individual reactivity from donor to donor was very large, the work was repeated taking into account the characteristics of the blood being studied.…”

Section: Chemiluminescence Of Human Granulocytesmentioning

confidence: 96%

Experimental study of homoeopathy in allergology: 2) Biological Studies

Poitevin¹

1998

Br Homeopath J

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Whole blood chemiluminescence as a systemic inflammatory parameter in asthma

Cited by 9 publications

References 35 publications

A survey of reactive oxygen species and their role in dermatology

A survey of reactive oxygen species and their role in dermatology

Particle‐induced myeloperoxidase release in serially diluted whole blood quantifies the number and the phagocytic activity of blood neutrophils and opsonization capacity of plasma

Experimental study of homoeopathy in allergology: 2) Biological Studies

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