Whole Cell PatchClamp Electrophysiology in Opsin-Expressing Brain Slice with Visible Lights or UCNPs

Si, Yueguang; Wang, Yiting

doi:10.21203/rs.3.pex-1627/v1

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“…We first investigated the chromatic selectivity of the three optogenetic proteins 5 . In an in vitro brain slice preparation, the protocol describing electrophysiology recording via UCNPs mediation can be found at Protocol Exchange 29 . It is confirmed that 470 nm stimulation elicited more spikes in ChR2-expressing cells than C1V1(T/T)-expressing cells (activation ratio~0.8:0.5) (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Near-infrared manipulation of multiple neuronal populations via trichromatic upconversion

Chen

Wang

et al. 2021

Nat Commun

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Using multi-color visible lights for independent optogenetic manipulation of multiple neuronal populations offers the ability for sophisticated brain functions and behavior dissection. To mitigate invasive fiber insertion, infrared light excitable upconversion nanoparticles (UCNPs) with deep tissue penetration have been implemented in optogenetics. However, due to the chromatic crosstalk induced by the multiple emission peaks, conventional UCNPs or their mixture cannot independently activate multiple targeted neuronal populations. Here, we report NIR multi-color optogenetics by the well-designed trichromatic UCNPs with excitation-specific luminescence. The blue, green and red color emissions can be separately tuned by switching excitation wavelength to match respective spectral profiles of optogenetic proteins ChR2, C1V1 and ChrimsonR, which enables selective activation of three distinct neuronal populations. Such stimulation with tunable intensity can not only activate distinct neuronal populations selectively, but also achieve transcranial selective modulation of the motion behavior of awake-mice, which opens up a possibility of multi-color upconversion optogenetics.

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Section: Resultsmentioning

confidence: 99%

Near-infrared manipulation of multiple neuronal populations via trichromatic upconversion

Chen

Wang

et al. 2021

Nat Commun

Self Cite

View full text Add to dashboard Cite

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Whole Cell PatchClamp Electrophysiology in Opsin-Expressing Brain Slice with Visible Lights or UCNPs

Abstract: It describes the flow of whole cell patch-clamp electrophysiology in mice brain slice.

Cited by 1 publication

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Near-infrared manipulation of multiple neuronal populations via trichromatic upconversion

Near-infrared manipulation of multiple neuronal populations via trichromatic upconversion

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