Whole-gene analysis of inter-genogroup reassortant rotaviruses from the Dominican Republic: Emergence of equine-like G3 strains and evidence of their reassortment with locally-circulating strains

Katz, Eric; Esona, Mathew D.; Betrapally, Naga S.; Leon, Lucia A. De La Cruz De; Neira, Yenny R.; Rey, Gloria; Bowen, Michael D.

doi:10.1016/j.virol.2019.06.007

Cited by 39 publications

(48 citation statements)

References 67 publications

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“…G3P[8] strains with a short RNA profile are described as DS‐1‐like (equine‐like) G3P[8] strains since their VP7 gene sequences are highly related to those of RVAs detected in horses . Recently, DS‐1‐like G1P[8] strains and equine‐like G3P[8] strains have spread worldwide …”

Section: Introductionmentioning

confidence: 99%

“…11,[13][14][15] Recently, DS-1-like G1P [8] strains and equine-like G3P [8] strains have spread worldwide. [8][9][10][11][12][14][15][16][17][18][19][20][21][22][23][24][25] In this study, we determined the G/P type distribution and carried out the full-genome characterization of two representative unusual equine-like G3P [8] strains which were highly prevalent (33.7%) in Thailand between January 2014 and September 2016. It is noteworthy that the equine-like G3P [8] strains with a short RNA profile that had been recently detected in several countries were predominant (65.3% to 89.8%) in various age groups across Thailand since 2015.…”

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confidence: 99%

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High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

Tacharoenmuang

Komoto

Guntapong

et al. 2019

Journal of Medical Virology

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Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine‐like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine‐like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine‐like and the other human‐like. Thus, we found that characteristic equine‐like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

Tacharoenmuang

Komoto

Guntapong

et al. 2019

Journal of Medical Virology

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“…Of note is that three (VP7, NSP1, and NSP2) and four (VP7, VP1, VP3, and NSP4) genes of strains DBM2017-016 and DBM2018-291, respectively, were assumed to have originated from locally circulating Wa-like G9P [8] and/or DS-1-like G2P [4] strains. Thus, the genomic diversity among Thai DS-1-like G9P [8] intergenogroup reassortant strains detected in the present study appeared to have been generated through additional reassortment with locally circulating RVAs [6,10,13,16,19,22,31,44]. It should also be noted that the VP7 gene of strain DBM2017-203 was closely related to that of contemporary French Wa-like G9P [8] strain Dijon-R10484, however, epidemiological links to Thailand were missing.…”

Section: Discussionmentioning

confidence: 56%

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

et al. 2020

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The emergence and rapid spread of unusual DS-1-like intergenogroup reassortant rotaviruses having G1/3/8 genotypes have been recently reported from major parts of the world (Africa, Asia, Australia, Europe, and the Americas). During rotavirus surveillance in Thailand, three novel intergenogroup reassortant strains possessing the G9P[8] genotype (DBM2017-016, DBM2017-203, and DBM2018-291) were identified in three stool specimens from diarrheic children. In the present study, we determined and analyzed the full genomes of these three strains. On full-genomic analysis, all three strains were found to share a unique genotype constellation comprising both genogroup 1 and 2 genes: G9-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis demonstrated that each of the 11 genes of the three strains was closely related to that of emerging DS-1-like intergenogroup reassortant, human, and/or locally circulating human strains. Thus, the three strains were suggested to be multiple reassortants that had acquired the G9-VP7 genes from co-circulating Wa-like G9P[8] rotaviruses in the genetic background of DS-1-like intergenogroup reassortant (likely equine-like G3P[8]) strains. To our knowledge, this is the first description of emerging DS-1-like intergenogroup reassortant strains having the G9P[8] genotype. Our observations will add to the growing insights into the dynamic evolution of emerging DS-1like intergenogroup reassortant rotaviruses through reassortment.

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“…have emerged and spread among human populations in at least five continents (Asia, Australia, Europe, North America, and South America) [11,12].…”

Section: ]-I2-r2-c2-m2-a2-n2-t2-e2-h2mentioning

confidence: 99%

“…In the genotyping system, the genotype constellation is described by the nomenclature Gx-P[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx, which defines the genotypes of VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NS P2-NSP3-NSP4-NSP5, respectively, with x indicating the number of corresponding genotypes [10]. At present, 36 G, 51 P, 26 I, 22 R, 20 C, 20 M, 31 A, 22 N, 22 T, 27 E and 22 H genotypes among the RVA have been identified in human and animal species worldwide [11]. To date, 6 G genotypes (G1-G4, G9, and G12) and 3 P genotypes (P [4], P [6], and P [8]) have been commonly identified within the G-P combinations of RVA.…”

Section: Introductionmentioning

confidence: 99%

High prevalence of DS-1-like rotavirus infection in Thai adults between 2016 and 2019

et al. 2020

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Rotavirus infection is the most common cause of viral diarrhea in infants and young children but uncommon and usually asymptomatic in adults. In the winter of 2017-2018, a largescale outbreak of rotavirus in both children and adults was reported in Thailand. The current study focused on the prevalence, genotyping, and molecular characterization of rotavirus infections in Thai adults from July 2016 to December 2019. In 2,598 stool samples collected from adult residents of Bangkok (aged #x2265; 15 years) with acute gastroenteritis, rotavirus was detected via real-time RT-PCR analysis of the VP6 gene. G, P and I genotypes were determined by direct sequencing of VP7, VP4, and VP6 genes, respectively. Our results showed 8.7% (226/2,598) of stool samples were positive for rotavirus. The incidence of rotavirus was high during the winter season of 2017-2018 (17.7%) compared to another studied periods (4.5% between July 2016-October 2017 and 2.8% between March 2018-December 2019). Nucleotide sequencing of VP7 and VP4 revealed G3P[8] as the predominant strain (33.2%,75/226), followed by G9P[8] (17.3%,39/226), and G2P[4] (15.0%,34/ 226). Uncommon G and P combinations were additionally detected at low frequencies. VP6 sequencing was conducted to discriminate I genotype between the Wa and DS-1 genogroup. The unusual DS-1-like G3P[8] strain was most prevalent amomg rotavirus strains detected in this study (29.6%, 67/226), and the corresponding VP7 sequences showed high nucleotide identity with unusual DS-1-like globally circulating strains. Our study demonstrates that rotavirus outbreaks in adults are attributable not only to high prevalence of RV infection but also the unusual DS-like genogroup. The collective findings reinforce the importance of investigating rotavirus diagnosis in adults suffering from acute gastroenteritis and taking appropriate preventive measures.

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Whole-gene analysis of inter-genogroup reassortant rotaviruses from the Dominican Republic: Emergence of equine-like G3 strains and evidence of their reassortment with locally-circulating strains

Cited by 39 publications

References 67 publications

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

High prevalence of DS-1-like rotavirus infection in Thai adults between 2016 and 2019

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