High prevalence of DS-1-like rotavirus infection in Thai adults between 2016 and 2019

Chansaenroj, Jira; Chuchaona, Watchaporn; Lestari, Fajar Budi; Pasittungkul, Siripat; Klinfueng, Sirapa; Wanlapakorn, Nasamon; Vongpunsawad, Sompong; Chirathaworn, Chintana; Poovorawan, Yong

doi:10.1371/journal.pone.0235280

Cited by 13 publications

(22 citation statements)

References 52 publications

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“…The incidence of rotavirus diarrhea in adults has been reported ranging from 2 to 22% of gastroenteritis cases in numerous countries [ 48 , 49 ]. In the present study, the incidence (12.3%) of RVA was higher than that in Thai adults (8.7%) [ 50 ]. It is suggested that rotavirus gastroenteritis in adult population should be also prevented by any intervention in China.…”

Section: Discussionmentioning

confidence: 53%

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

et al. 2020

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Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. To learn the shift of genotypes and genetic characteristics of Rotavirus A (RVA) causing diarrhea in children and adults, a hospital-based surveillance of rotavirus was conducted in Wuhan, China from June 2011 through May 2019, and representative virus strains were phylogenetically analyzed. Among a total of 6733 stool specimens collected from both children and adults with acute gastroenteritis, RVA was detected in 25.5% (1125/4409) and 12.3% (285/2324) of specimens, respectively. G9P[8] was the most common genotype (74.5%), followed by G1P[8] (8.7%), G2P[4] (8.4%), and G3P[8] (7.3%), with G9P[8] increasing rapidly during the study period. The predominant genotype shifted from G1P[8] to G9P[8] in 2012–2013 epidemic season. G12P[6] strain RVA/Human-wt/CHN/Z2761/2019/G12P[6] was detected in April 2019 and assigned to G12-P[6]-I1-R1-C1-M1-A1-N1-T2-E1-H1 genotypes. Phylogenetic analysis revealed that VP7, VP4, VP6, VP3, NSP1, NSP2, and NSP5 genes of Z2761 clustered closely with those of Korean G12P[6] strain CAU_214, showing high nucleotide identities (98.0–98.8%). The NSP3 gene of Z2761 was closely related to those of G2P[4] and G12P[6] rotaviruses in Asia. All the eleven gene segments of Z2761 kept distance from those of cocirculating G9P[8], G1P[8], and G3P[8] strains detected in Wuhan during this study period. This is the first identification of G12 rotavirus in China. It is deduced that Z2761 is a reassortant having DS-1-like NSP3 gene in the background of G12P[6] rotavirus genetically close to CAU_214.

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Section: Discussionmentioning

confidence: 53%

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

et al. 2020

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“…Among the 2,001 stool samples obtained from infants and young patients with AGE over a 4-year study period (January 2016 to December 2019), 301 samples (15.0%) tested positive for RVA. Interestingly, there was a large-scale outbreak of RVA infection in both children and adult between December 2017 and March 2018 ( Chansaenroj et al, 2020 ). The highest frequency of RVA infection in this study occurred in children aged 0–24 months (48.2%), which corresponded to data from a previous study in Mexico, in which almost all of the children by 2 years of age had been infected at least once, and more than two-thirds had experienced at RVA least twice, and more than 10% had experienced five infections ( Velázquez et al, 1996 ).…”

Section: Discussionmentioning

confidence: 99%

“…The primers VP6-F (5′ GACGGVGCRACTACATGGT 3′) and VP6-R (5′ GTCCAATTCATNCCTGGTGG 3′) a 379-bp region corresponding to nucleotides 747–1,126 of the VP6 gene. Cycling parameters were reverse transcription at 50 °C for 30 min, initial denaturation at 95 °C for 15 min, 45 cycles of denaturation at 94 °C for 15 s, annealing at 60 °C for 30 s, and extension at 72 °C for 30 s. Melting curve analysis was explored from 60 °C to 95 °C with 1 °C increments to determine the specificity of the reactions ( Kang et al, 2004 ; Chansaenroj et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…The RT-PCR conditions for VP6 gene comprised the reverse transcription step at 45 °C for 45 min, followed by initial denaturation at 95 °C for 5 min, 40 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, extension at 72 °C for 2 min, and the reaction concluded with a final extension at 72 °C for 10 min. The RT-PCR conditions of VP7 and VP4 genes were: reverse transcription at 45 °C for 30 min, followed by initial denaturation 94 °C for 5 min, 40 cycles of denaturation at 94 °C for 45 s, annealing at 48 °C (for BEG9 and END9) or 55 °C (for con2 and con3) for 30 s, extension at 68 °C for 1 min, and included a final extension step at 68 °C for 5 min ( Chansaenroj et al, 2020 ). The expected amplicons were agarose gel-purified and sequenced by FirstBASE Laboratories (SDN BHD, Selangor, Malaysia).…”

Section: Methodsmentioning

confidence: 99%

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High prevalence of circulating DS-1-like human rotavirus A and genotype diversity in children with acute gastroenteritis in Thailand from 2016 to 2019

Pasittungkul

Lestari

Puenpa

et al. 2021

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Background Human rotavirus A (RVA) infection is the primary cause of acute gastroenteritis (AGE) in infants and young children worldwide, especially in children under 5 years of age and is a major public health problem causing severe diarrhea in children in Thailand. This study aimed to investigate the prevalence, genotype diversity, and molecular characterization of rotavirus infection circulating in children under 15 years of age diagnosed with AGE in Thailand from January 2016 to December 2019. Methods A total of 2,001 stool samples were collected from children with gastroenteritis (neonates to children <15 years of age) and tested for RVA by real-time polymerase chain reaction (RT-PCR). Amplified products were sequenced and submitted to an online genotyping tool for analysis. Results Overall, 301 (15.0%) stool samples were positive for RVA. RVA occurred most frequently among children aged 0-24 months. The seasonal incidence of rotavirus infection occurred typically in Thailand during the winter months (December-March). The G3P[8] genotype was identified as the most prevalent genotype (33.2%, 100/301), followed by G8P[8] (10.6%, 32/301), G9P[8] (6.3%, 19/301), G2P[4] (6.0%, 18/301), and G1P[6] (5.3%, 16/301). Uncommon G and P combinations such as G9P[4], G2P[8], G3P[4] and G3P[9] were also detected at low frequencies. In terms of genetic backbone, the unusual DS-1-like G3P[8] was the most frequently detected (28.2%, 85/301), and the phylogenetic analysis demonstrated high nucleotide identity with unusual DS-1-like G3P[8] detected in Thailand and several countries. Conclusions A genetic association between RVA isolates from Thailand and other countries ought to be investigated given the local and global dissemination of rotavirus as it is crucial for controlling viral gastroenteritis, and implications for the national vaccination programs.

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“…In Russia, this genotype combination was detected previously in the Siberian region [28]. Viruses with the G8P [8] genotype combination have been detected in people in Africa and Asia, where they cause disease not only in children, but also in adults [29,34]. Viruses with the G9P [4] combination are widespread in Asia and South America, though sometimes they are detected in Europe [35].…”

Section: Discussionmentioning

confidence: 78%

Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

et al. 2021

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Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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High prevalence of DS-1-like rotavirus infection in Thai adults between 2016 and 2019

Cited by 13 publications

References 52 publications

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

High prevalence of circulating DS-1-like human rotavirus A and genotype diversity in children with acute gastroenteritis in Thailand from 2016 to 2019

Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

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