Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces

Stangegaard, Michael; Wang, Z.; Kutter, Joerg P.; Dufva, Martin; Wolff, Anders

doi:10.1039/b608239d

Cited by 44 publications

(46 citation statements)

References 51 publications

(61 reference statements)

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“…Such simple and robust surface modifications can have great impact for the use of SU-8 in e.g. DNA microarrays 34 , microfluidics 35 or for cell device applications 39 as also presented here.…”

Section: -15 |mentioning

confidence: 99%

Tunable high aspect ratio polymer nanostructures for cell interfaces

et al. 2015

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Nanoscale topographies and chemical patterns can be used as synthetic cell interfaces with a range of applications including study and control of cellular processes. Herein, we describe the fabrication of high aspect ratio nanostructures using electron beam lithography in the epoxy-based polymer SU-8. We show how nanostructure geometry, position and fluorescent properties can be tuned, allowing flexible device design. Further, thiol-epoxide reactions were developed to give effective and specific modification of SU-8 surface chemistry. SU-8 nanostructures were made directly on glass cover slips, enabling the use of high resolution optical techniques such as live-cell confocal, total internal reflection and 3D structured illumination microscopy to investigate cell interactions with the nanostructures. Details of cell adherence and spreading, plasma membrane conformation and actin organization in response to high aspect ratio nanopillars and nanolines were investigated. The versatile structural and chemical properties combined with high resolution cell imaging capabilities of this system are an important step towards better understanding and controlling cell interactions with nanomaterials.

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Section: -15 |mentioning

confidence: 99%

Tunable high aspect ratio polymer nanostructures for cell interfaces

et al. 2015

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“…One approach for wet chemical hydrophilization is based on etching by ceric ammonium nitrate followed by coating with ethanolamine. [20,21] Plasma processes can introduce specific binding sites or modify the polymer surface by cross-linking, chainscission, or incorporation of functionalities. [22,23] Oxygen plasma treatment, for example, renders the SU-8 surface hydrophilic by generating C O and COO groups at the surface, [19] increases the surface roughness, [24] and enhances cell proliferation on the surface.…”

Section: Introductionmentioning

confidence: 99%

Surface hydrophilization of SU‐8 by plasma and wet chemical processes

Walther

Drobek

Gigler

et al. 2010

Surface & Interface Analysis

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Wetting properties and surface roughness of SU-8 can be modified by wet chemical and plasma processes. These processes result in an enhanced wettability that can be attributed to an increase of C O and COO groups at the surface. Wet chemical etching with ceric ammonium nitrate (CAN) rendered the SU-8 surface hydrophilic. However, it also led to the accumulation of cerium species on the surface, which may interfere with biochemical reactions in the device. Surface activation could also be achieved with a low-temperature atmospheric argon plasma, which left a smooth surface. Treatment of SU-8 with oxygen plasma led to a stable hydrophilization and an increased surface roughness. Owing to oxygen plasma processing, antimony species were accumulated which could be removed with a cleaning step. In order to maintain the hydrophilization after oxygen plasma treatment for storage, or over several wetting cycles, the surface can be coated with a protein-resistant graft copolymer (PLL-g-PEG).

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“…The chemically 23 and thermally 24 stable as well as optically transparent material has been employed for the fabrication of devices used for drug delivery 25 and single cell manipulation 26 as well as for biosensor chips 27 and microarrays. 28,29 The suitability for implantable medical devices has been reported by Kotzar 30 and demonstrated with microneedles for neural recording. 31 Kalkandjiev 22 reported on the biocompatibility of dry-resist on epoxy-basis.…”

Section: A Dry-resist Processingmentioning

confidence: 96%

Complex three-dimensional high aspect ratio microfluidic network manufactured in combined PerMX dry-resist and SU-8 technology

et al. 2011

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In this paper we present a new fabrication method that combines for the first time popular SU-8 technology and PerMX dry-photoresist lamination for the manufacturing of high aspect ratio three-dimensional multi-level microfluidic networks. The potential of this approach, which further benefits from wafer-level manufacturing and accurate alignment of fluidic levels, is demonstrated by a highly integrated three-level microfluidic chip. The hereby achieved network complexity, including 24 fluidic vias and 16 crossing points of three individual microchannels on less than 13 mm 2 chip area, is unique for SU-8 based fluidic networks. We further report on excellent process compatibility between SU-8 and PerMX dryphotoresist which results in high interlayer adhesion strength. The tight pressure sealing of a fluidic channel (0.5 MPa for 1 h) is demonstrated for 150 lm narrow SU-8/PerMX bonding interfaces.

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Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces

Cited by 44 publications

References 51 publications

Tunable high aspect ratio polymer nanostructures for cell interfaces

Tunable high aspect ratio polymer nanostructures for cell interfaces

Surface hydrophilization of SU‐8 by plasma and wet chemical processes

Complex three-dimensional high aspect ratio microfluidic network manufactured in combined PerMX dry-resist and SU-8 technology

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