Whole-genome regulation analysis of histone H3 lysin 27 trimethylation in subclinical mastitis cows infected by Staphylococcus aureus

Abstract

BackgroundS. aureus is one of the major etiological agents causing bovine subclinical mastitis. The regulatory effects of H3K27me3 on gene expression in subclinical S. aureus mastitis cows are unknown. This study aimed to profile genome-wide transcriptional changes regulated by H3K27me3 in bovine lymphocytes applied in subclinical S. aureus mastitis cows and healthy controls.ResultsA total of 61 differentially expressed genes (DEGs) were detected in subclinical S. aureus mastitis cows compared to the healthy controls, of which 25 DEGs are up-regulated and the rest are down-regulated genes in subclinical S.aureus mastitis cows. The up-regulated genes are mainly involved in the Jak-STAT signaling pathway, cytokine-cytokine receptor interaction, and T cell receptor-signaling pathway, while the down-regulated genes are related to metabolism pathways. Combination analysis of histone methylation and gene expression revealed that H3K27 trimethylation levels in silent genes were higher in subclinical S. aureus mastitis cattle than in healthy cows. The key regions of H3K27me3 target genes related to subclinical S. aureus mastitis were the upstream 2 kb regions of the DEGs relative to transcription start site (TSS).ConclusionsThe current study provides a novel insight into the interaction between S. aureus and lymphocytes in lactating cows by histone H3 methylation regulation. The differentially expressed genes in bovine lymphocytes regulated by H3K27me3 on upstream 2 kb regions (IL10, PTX3 and etc.) may relate to S. aureus mastitis susceptibility and could be considered as key candidate genes for anti- S. aureus mastitis study and breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2947-0) contains supplementary material, which is available to authorized users.

Genome-Wide Transcriptional and Post-transcriptional Regulation of Innate Immune and Defense Responses of Bovine Mammary Gland to Staphylococcus aureus

“…Chemokine signaling pathway plays important roles in regulating and mobilizing immune-relevant cells, and is involved in providing directional cues for leukocytes to the site of inflammation (Mellado et al, 2001), indicating the importance of recruitment and activation of immune cells to sites of infection during acute S. aureus mastitis. Noticeably, several other pathways primarily involved in pro-inflammatory and innate immune system were uncovered in the present study but not in Wang et al (2013), including cytokine-cytokine receptor interaction, Jak-STAT signaling pathway (FDR = 0.05; Figure S5) and Toll-like receptor signaling pathway, all of which were also demonstrated to be associated with S. aureus mastitis in both dairy cows and goats by previous studies (Pisoni et al, 2010; He et al, 2016). However, several human neurological disease-related pathways (e.g., Huntingson's, Alzheimer's, and Parkinson's diseases) were only observed in Wang et al (2013).…”

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

“…For more understanding, we conducted the transcriptomic study of PBLs for the three dairy cattle groups with high, low and none folic acid supplementation. Previous studies reported that many biological pathways play a key role by accelerating immune-relevant cells and provide a strong defense against any foreign pathogens [ 21 ]. The nuclear factor (NF)-kappa B signaling pathway, hematopoietic cell lineage, and TNF signaling pathways were reported to respond to Staphylococcus aureus ( S. aureus ) in mammary epithelial cells [ 22 ], and against porcine reproductive and respiratory syndrome virus post-vaccination in pregnant sows [ 23 ].…”

Genome-Wide DNA Methylation Analysis of Mammary Gland Tissues From Chinese Holstein Cows With Staphylococcus aureus Induced Mastitis

“…T cells activated by monocytederived dendritic cells play key roles in immune response to S. aureus (Bharathan, 2016). He et al (2016) also reported that differently expressed genes due to subclinical S. aureus mastitis in cattle are involved in the T cell receptor-signaling pathway. These reports are supported by the enriched GO terms related to the immune response regulation and immune system, and KEGG disease and immune related pathways observed in this study.…”

“…Furthermore, growing evidence demonstrates that epigenetics also profoundly influence health, growth, development and production traits of cattle (Ibeagha-Awemu and Reynolds et al, 2017;Sun et al, 2019). Several studies have reported the potential contribution of epigenetic mechanisms like DNA methylation (Song et al, 2016;Zhang et al, 2018), histone modification (He et al, 2016) and non-coding RNA (Li et al, 2015;Pu et al, 2017;Ma et al, 2019) to mastitis risk. As the most characterized epigenetic regulatory mechanism, DNA methylation was reported to regulate mammary gland health (Zhang et al, 2018;Chen et al, 2019;Wang et al, 2019).…”