2016
DOI: 10.1016/j.gdata.2016.05.008
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Whole genome sequence analysis of an Alachlor and Endosulfan degrading Pseudomonas strain W15Feb9B isolated from Ochlockonee River, Florida

Abstract: We recently isolated a Pseudomonas sp. strain W15Feb9B from Ochlockonee River, Florida and demonstrated potent biodegradative activity against two commonly used pesticides - Alachlor [(2-chloro-2′,6′-diethylphenyl-N (methoxymethyl)acetanilide)] and Endosulfan [(6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,3,4-benzo(e)di-oxathiepin-3-oxide], respectively. To further identify the repertoire of metabolic functions possessed by strain W15Feb9B, a draft genome sequence was obtained, assembled, anno… Show more

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Cited by 20 publications
(7 citation statements)
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“…Briefly, genomic DNA from the strain was extracted and prepared for sequencing on an Illumina HiSeq2000 instrument as described previously [5], [6]. De novo assembly of the raw reads was performed with the SPAdes assembler [7] using default settings.…”
Section: Textmentioning
confidence: 99%
“…Briefly, genomic DNA from the strain was extracted and prepared for sequencing on an Illumina HiSeq2000 instrument as described previously [5], [6]. De novo assembly of the raw reads was performed with the SPAdes assembler [7] using default settings.…”
Section: Textmentioning
confidence: 99%
“…For example, in P. aeruginosa strains F9676 and DN1, P. alcaliphila strain JAB1, and Pseudomonas sp. strain W15Feb9B, the definite functional categories containing the largest number of genes are amino acid transport and metabolism, signal transduction mechanisms, transcription (Shi et al, 2015; Chauhan et al, 2016; Dong et al, 2017; Ridl et al, 2018), which are also top enriched categories in our tested strains. Thus, the genomic features responsible for AFB1 degradation should not be inherent elements of the genomes of genus Pseudomonas .…”
Section: Discussionmentioning
confidence: 77%
“…Strains from Pseudomonas , Achromobacter or Stenotrophomonas genera which proliferated in presence of dimethachlor were previously reported to be able to degrade various acetanilide herbicides like alachlor 43 , acetochlor 44 , butolachlor 45 . Microorganisms capable of degrading herbicide are commonly also hyper-tolerant to this herbicide 46 which suggest finding dimethachlor degraders among groups which proliferated.…”
Section: Discussionmentioning
confidence: 99%