Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades

Abstract

Salmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. Dublin strains isolated in Brazil and in other countries were analyzed to determine their phylogenetic relationships, the presence of genes, plasmids, genomic regions related to virulence and antimicrobial resistance genes repertoire, using WGS analyses. Illumina was used to sequence the genome of 112 S. Dublin strains isolated in Brazil from humans (n = 82) and animals (n = 30) between 1983 and 2016. Furthermore, 87 strains from other countries were analyzed. WGSNP analysis revealed three different clades, in which the strains from Brazil belonged to two clades, A and C. Most of the genes and genomic regions searched varied among the strains studied. The siderophore genes iroB and iroC were exclusively found in strains from Brazil and pegD gene, related to fimbrial adherence determinants, were positive in 124 strains from clades A and B but absent in all the strains from clade C (n = 71). Eleven plasmid replicons were found in the strains from Brazil, and nine were exclusively found in strains from other countries. The antimicrobial resistance genes mdsA and mdsB, that encode an efflux pump, were found in all the strains studied. The strains from Brazil carried other resistance genes, such as tet(A) (n = 11), tet(B) (n = 4) and tet(C) (n = 4), blaTEM-1 (n = 4), catA1 (n = 1), aadA1 (n = 1), and sul1 (n = 1). In conclusion, S. Dublin strains isolated in Brazil presented some few unique genes not found in strains from other countries and were allocated into two distinct clades with strains of human and animal origin epidemiologically related. This fact stresses the zoonotic potential of S. Dublin circulating in Brazil for more than 30 years.

Prevalence and Characteristics of Salmonella spp. Isolated from Raw Chicken Meat in the Republic of Korea

“…isolated from domestic chicken sold in Chungcheong Province showed specific sequence types as the ST of Salmonella spp., which several studies reported were isolated from Brazilian poultry. Salmonella Typhimurium isolated from poultry revealed ST-19 [ 41 , 42 ] and most of the Salmonella Dublin isolates ( n = 112) from human and animal presented ST-10 ( n = 68), ST-3734 ( n = 28), and ST-4030 ( n = 9) [ 43 ].…”

Clonal relation between Salmonella enterica subspecies enterica serovar Dublin strains of bovine and food origin in Germany

“…The detection of ST-10 in human-derived strains from Denmark ( 7 ) indicates that strains from this ST are able to cause human infections. This assumption is further supported by the detection of ST-10 in human patients from Brazil, Canada, UK, and USA ( 20 ).…”

Antimicrobial Resistance and Genomic Characterization of Salmonella Isolated from Pigeons in China

“…MdsA and mdsB can promote resistance to various antibacterial drugs, including phenicol antibiotic, cephalomycin, monobactam, carbapenem, and cephalosporin. This indicates that its presence can lead to resistance to multiple antibiotics [37][38][39][40]. Furthermore, partial regulatory systems contribute to bacterial resistance to heavy metals.…”