Whole-Genome Shotgun Sequencing of Rhodococcus erythropolis Strain P27, a Highly Radiation-Resistant Actinomycete from Antarctica

Taketani, Rodrigo Gouvêa; Zucchi, Tiago Domingues; Melo, I. S. de; Mendes, Rodrigo

doi:10.1128/genomea.00763-13

Cited by 10 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antarctica has become an interesting site for bioprospecting microorganisms with biotechnological purposes. This extremely harsh environment usually poses numerous threats to living beings ( 1 ) and, notwithstanding, may select organisms that held unique biosynthetic pathways ( 2 ). In our survey at Stain House Lake, we isolated bacteria able to degrade cellulose ( 3 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Bacillus sp. Strain CMAA 1185, a Cellullolytic Bacterium Isolated from Stain House Lake, Antarctic Peninsula

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Bacillus sp. Strain CMAA 1185, a Cellullolytic Bacterium Isolated from Stain House Lake, Antarctic Peninsula

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Sequencing was carried out on the Ion 316 chip sequencer provided in the Ion sequencing kit 200 bp version 2.0, according to the manufacturer’s protocol. The genome sequence was de novo assembled using the MIRA version 3.4, CLC Genomics Workbench version 5.5.1, and SeqMan NGen version 4.0.0 packages, and the contigs obtained from the assembly were integrated using CISA ( 3 , 4 ). The taxonomic position of strain CMAA 1215 was further evaluated by using the JSpecies package ( 5 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Pseudomonas sp. Strain CMAA 1215, a Plant Growth-Promoting Bacterium Isolated from a Brazilian Mangrove

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…deserti and D . murrayi ) and other bacteria including Rubrobacter radiotolerans [ 14 ] and Micrococcus luteus [ 16 ] depend highly on RecFOR system for recombinational repair of DNA while others such as Keinococcus radiotolerans [ 27 ], Rhodococcus erythropolis [ 28 ] and Promicromonospora panici [ 17 ] show the presence of both RecFOR and RecBCD pathways. Yet another variety, Methylobacterium radiodurans , depends only on RecBCD pathway [ 22 ] for repair mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Unraveling radiation resistance strategies in two bacterial strains from the high background radiation area of Chavara-Neendakara: A comprehensive whole genome analysis

Pal,

Yuvaraj,

Krishnan

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

This paper reports the results of gamma irradiation experiments and whole genome sequencing (WGS) performed on vegetative cells of two radiation resistant bacterial strains, Metabacillus halosaccharovorans (VITHBRA001) and Bacillus paralicheniformis (VITHBRA024) (D10 values 2.32 kGy and 1.42 kGy, respectively), inhabiting the top-ranking high background radiation area (HBRA) of Chavara-Neendakara placer deposit (Kerala, India). The present investigation has been carried out in the context that information on strategies of bacteria having mid-range resistance for gamma radiation is inadequate. WGS, annotation, COG and KEGG analyses and manual curation of genes helped us address the possible pathways involved in the major domains of radiation resistance, involving recombination repair, base excision repair, nucleotide excision repair and mismatch repair, and the antioxidant genes, which the candidate could activate to survive under ionizing radiation. Additionally, with the help of these data, we could compare the candidate strains with that of the extremely radiation resistant model bacterium Deinococccus radiodurans, so as to find the commonalities existing in their strategies of resistance on the one hand, and also the rationale behind the difference in D10, on the other. Genomic analysis of VITHBRA001 and VITHBRA024 has further helped us ascertain the difference in capability of radiation resistance between the two strains. Significantly, the genes such as uvsE (NER), frnE (protein protection), ppk1 and ppx (non-enzymatic metabolite production) and those for carotenoid biosynthesis, are endogenous to VITHBRA001, but absent in VITHBRA024, which could explain the former’s better radiation resistance. Further, this is the first-time study performed on any bacterial population inhabiting an HBRA. This study also brings forward the two species whose radiation resistance has not been reported thus far, and add to the knowledge on radiation resistant capabilities of the phylum Firmicutes which are abundantly observed in extreme environment.

show abstract

Whole-Genome Shotgun Sequencing of Rhodococcus erythropolis Strain P27, a Highly Radiation-Resistant Actinomycete from Antarctica

Abstract: Here, we report the draft genome sequence of radiation-resistant Rhodococcus erythropolis strain P27, isolated from leaves of Deschampsia antarctica Desv. (Poaceae) in the Admiralty Bay area, Antarctica.

Cited by 10 publications

References 9 publications

Draft Genome Sequence of Bacillus sp. Strain CMAA 1185, a Cellullolytic Bacterium Isolated from Stain House Lake, Antarctic Peninsula

Draft Genome Sequence of Bacillus sp. Strain CMAA 1185, a Cellullolytic Bacterium Isolated from Stain House Lake, Antarctic Peninsula

Draft Genome Sequence of Pseudomonas sp. Strain CMAA 1215, a Plant Growth-Promoting Bacterium Isolated from a Brazilian Mangrove

Unraveling radiation resistance strategies in two bacterial strains from the high background radiation area of Chavara-Neendakara: A comprehensive whole genome analysis

Contact Info

Product

Resources

About