Whole Root Transcriptomic Analysis Reveals a Role for Auxin Pathways in Resistance to<i>Ralstonia solanacearum</i>in Tomato

French, Elizabeth; Kim, Bong Suk; Rivera-Zuluaga, Katherine; Iyer‐Pascuzzi, Anjali S.

doi:10.1101/176685

Cited by 6 publications

(5 citation statements)

References 84 publications

(134 reference statements)

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“…In line with our data, the expression of DR5, a marker gene of the auxin signaling pathway, was strongly induced in root vascular after R. solanacearum GMI1000 infection (Lu et al 2018). Moreover, the dgl1-1 tomato mutant with disordered auxin transport was highly resistant to R. solanacearum (French et al 2018). Together, these data strongly support that auxin signaling plays a negative role in root defense against R. solanacerum.…”

Section: R Solanacearum Causes Genome-wide Transcriptional Reprogramsupporting

confidence: 89%

Deep Sequencing Reveals Early Reprogramming of Arabidopsis Root Transcriptomes Upon Ralstonia solanacearum Infection

Wang

et al. 2019

MPMI

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Bacterial wilt caused by the bacterial pathogen Ralstonia solanacearum is one of the most devastating crop diseases worldwide. The molecular mechanisms controlling the early stage of R. solanacearum colonization in the root remain unknown. Aiming to better understand the mechanism of the establishment of R. solanacearum infection in root, we established four stages in the early interaction of the pathogen with Arabidopsis roots and determined the transcriptional profiles of these stages of infection. A total 2,698 genes were identified as differentially expressed genes during the initial 96 h after infection, with the majority of changes in gene expression occurring after pathogen-triggered root-hair development observed. Further analysis of differentially expressed genes indicated sequential activation of multiple hormone signaling cascades, including abscisic acid (ABA), auxin, jasmonic acid, and ethylene. Simultaneous impairment of ABA receptor genes promoted plant wilting symptoms after R. solanacearum infection but did not affect primary root growth inhibition or root-hair and lateral root formation caused by R. solanacearum. This indicated that ABA signaling positively regulates root defense to R. solanacearum. Moreover, transcriptional changes of genes involved in primary root, lateral root, and root-hair formation exhibited high temporal dynamics upon infection. Taken together, our results suggest that successful infection of R. solanacearum on roots is a highly programmed process involving in hormone crosstalk.*The e-Xtra logo stands for "electronic extra" and indicates that six supplementary figures, supplementary methods, two supplementary tables, and three datasets are published online.The author(s) declare no conflict of interest.

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Section: R Solanacearum Causes Genome-wide Transcriptional Reprogramsupporting

confidence: 89%

Deep Sequencing Reveals Early Reprogramming of Arabidopsis Root Transcriptomes Upon Ralstonia solanacearum Infection

Wang

et al. 2019

MPMI

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“…The gene Araip.ZD3PG was predicted to code for auxin response factor, which may involve in the regulation of auxin pathway. Recent reports showed that the repression of auxin pathway improved the resistance to bacterial pathogen (French et al, 2018;Navarro, 2016). The gene Araip.CMC8E might code for calcium-dependent protein kinase, which could perceive changes in intracellular calcium concentrations to transmit defence signal in response to PAMPs and pathogen effectors (Liu et al, 2017;Wang et al, 2018a).…”

Section: Discussionmentioning

confidence: 99%

Next‐generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut (Arachis hypogaea L.)

Luo

Pandey

Khan

et al. 2019

Plant Biotechnology Journal

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Summary Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics‐assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic markers. Here, we deployed sequencing‐based trait mapping approach, QTL‐seq, to discover genomic regions, candidate genes and diagnostic markers for BWR in a recombination inbred line population (195 progenies) of peanut. The QTL‐seq analysis identified one candidate genomic region on chromosome B02 significantly associated with BWR. Mapping of newly developed single nucleotide polymorphism (SNP) markers narrowed down the region to 2.07 Mb and confirmed its major effects and stable expressions across three environments. This candidate genomic region had 49 nonsynonymous SNPs affecting 19 putative candidate genes including seven putative resistance genes (R‐genes). Two diagnostic markers were successfully validated in diverse breeding lines and cultivars and could be deployed in genomics‐assisted breeding of varieties with enhanced BWR.

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“…The work of Tarinee et al [59] has shown that the auxin secreted by R. solanacearum is involved in the early stages of infection in tomatoes and accentuates the symptoms of the disease; they have demonstrated that several auxin signaling and transport mutants induce tomato resistance to bacterial wilt. In addition, the work of French et al [60] reported that during in vitro inoculation of R. solanacearum, effector proteins (T3SS and T3E) called type III effectors, whose expression is regulated by the bacterial protein HrpB, are the main determinants of the pathogenicity of the bacterium responsible for the various morphological alterations and the blocking of the growth of the primary roots of host plants with induction of disease symptoms [61].…”

Section: Grammentioning

confidence: 99%

Morphological and Biochemical Characterization of Strains of Ralstonia solanacearum, Causal Agent of Bacterial Wilt in Tomatoes in Cameroon and Screening of Virulent Strains

Mountseng,

Mahot,

Membang

et al. 2024

ARRB

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Ralstonia solanacearum is a major constraint in tomato production. The aim of this work was to identify the different biovars and races of R. solanacearum which infect tomato plants in Cameroon and determine the most virulent in a gnotobiotic environment. Thirty (30) samples were collected in the field of 03 different agro-ecological zones considered as major tomato production area in Cameroon. The bacteria were isolated on modified Kelman solid medium, and identified based on morphological and biochemical characterization. The pathogenicity test was performed in a gnotobiotic environment by root inoculation. Twenty-nine (29) isolates showed colonies characteristic of virulent strains. Water, motility, catalase, KOH, Kovac oxidase, glucose reduction, and sucrose tests were positive. However, gram staining, spore production, arginine test and sulfate reduction were negative. The biovar tests carried out revealed the presence16.66% biovar 2 (bv 2) and 76.66% biovar 3(bv 3). The tobacco hypersensitivity tests carried out revealed the presence of two (02) races: race 1 and race 3 (majority). A predominance of race3 biovar 3 was observed in Cameroon. Results from pathogenicity test revealed that two (02) strains, FM6 (race 3 bv2) and BFo (race 3 bv 3) were high virulent, causing up to 100% loss of seedlings on the sixth day post-inoculation.

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Whole Root Transcriptomic Analysis Reveals a Role for Auxin Pathways in Resistance toRalstonia solanacearumin Tomato

Abstract: Summary 24The soilborne pathogen Ralstonia solanacearum is the causal agent of bacterial wilt, and causes

Cited by 6 publications

References 84 publications

Deep Sequencing Reveals Early Reprogramming of Arabidopsis Root Transcriptomes Upon Ralstonia solanacearum Infection

Deep Sequencing Reveals Early Reprogramming of Arabidopsis Root Transcriptomes Upon Ralstonia solanacearum Infection

Next‐generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut (Arachis hypogaea L.)

Morphological and Biochemical Characterization of Strains of Ralstonia solanacearum, Causal Agent of Bacterial Wilt in Tomatoes in Cameroon and Screening of Virulent Strains

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