Abstract-The study was to develop, explore on effectiveness of an electronic learning (e-learning) on Anatomy and Physiology I on a "Muscular system", and investigate in learners' satisfaction related to the e-learning. Subjects were freshmen nursing students at Boromarajonani College of Nursing Uttaradit in Fall 2012 (n=98). Questionnaires were the e-learning on Anatomy and Physiology I on the "Muscular system", pre-test, practiced exercise, post-test, and learners' satisfaction. Statistical analyses were utilized on frequency, percentage, mean, and paired-t test (p < .05). Findings revealed that mean of ratio of score from pre-test before learning with the e-learning and post-test after utilizing on the e-learning with full score equaled 0.825 and 0.897, respectively. The percentage of effectiveness of e-learning on Anatomy and Physiology I on the "Muscular system" was 86.50 in a good level. Overall mean of learners' satisfaction (X=4.02) on the e-learning and each component on utilization (X=4.12), presentation (X=4.05), content (X=3.92), and learning evaluation (X=3.81) placed on the very good level, respectively. After nursing students participated in utilizing the e-learning, learning achievement was statistically significant higher than before studying on the e-learning (p < .05).Index Termsanatomy and physiology.