Wickerhamomyces xylosica sp. nov. and Candida phayaonensis sp. nov., two xylose-assimilating yeast species from soil

Abstract

Two strains (NT29T and NT31T) of xylose-assimilating yeasts were obtained from soils collected in northern Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were found to represent two novel ascomycete yeast species. Strain NT29T was assigned to the genus Candida belonging to the Pichia clade as a representative of Candida phayaonensis sp. nov.; the type strain is NT29T ( = BCC 47634T = NBRC 108868T = CBS 12319T). Strain NT31T represented a novel Wickerhamomyces species, which was named Wickerhamomyces xylosica sp. nov.; the type strain is NT31T ( = BCC 47635T = NBRC 108869T = CBS 12320T).

Wickerhamomyces mori sp. nov., an anamorphic yeast species found in the guts of wood-boring insect larvae

“…At present, there are many yeast species in the Wickerhamomyces clade. The species in this clade have been isolated from a wide variety of substrates, such as soil, seawater, plant substrates and animal-associated samples (Limtong et al , 2009, 2012; Groenewald et al , 2011; Kurtzman, 2011). Some species of the clade were isolated from insects and insect frass; for example, a strain of C .…”

“…Eight anamorphic Candida species, Candida mycetangii , Candida odintsovae , Candida peoriensis , Candida ponderosae , Candida quercuum , Candida silvicultrix , Candida solani and Candida ulmi , were also related to this genus (Kurtzman et al , 2008; Shin et al , 2011). Later described species in the genus Wickerhamomyces include Wickerhamomyces chaumierensis (Groenewald et al , 2011), Wickerhamomyces edaphicus (Limtong et al , 2009), Wickerhamomyces queroliae (Rosa et al , 2009), Wickerhamomyces patagonicus (de García et al , 2010), Wickerhamomyces ochangensis (Shin et al , 2011) and Wickerhamomyces xylosica (Limtong et al , 2012). Recognition of the genus Wickerhamomyces from phenotypic characteristics alone is somewhat difficult due to the restricted responses in standard physiological tests as well as limited morphological differences between the genus and closely related genera (Kurtzman et al , 2008).…”

Wickerhamomyces spegazzinii sp. nov., an ascomycetous yeast isolated from the fungus garden of Acromyrmex lundii nest (Hymenoptera: Formicidae)

“…Some characteristics of the genus Wickerhamomyces are the formation of hat-shaped ascospores or spherical ascospores with an equatorial ledge; cell division is by multilateral budding; some species form pseudohyphae and true hyphae; asci are globose to ellipsoid, and unconjugated; some species ferment glucose and they do not assimilate methanol or hexadecane; and the diazonium blue B reaction is negative (Kurtzman et al, , 2011a. Species currently accepted in the genus have been isolated from soil (Limtong et al, 2009;Kurtzman et al, 2011a;Shin et al, 2011;Limtong et al, 2012), plant material (Groenewald et al, 2011;Kaewwichian et al, 2013;James et al, 2014), tree exudates (Kurtzman et al, 2011a;de García et al, 2010), flowers (Nakase et al, 2012;James et al, 2014), digestive tract of insects (Hui et al, 2013;James et al, 2014), insect frass (Kurtzman et al, 2011a), larvae of diptera (Rosa et al, 2009), birds (Francesca et al, 2013), natural fermentation of coffee cherries (Silva et al, 2000) and brined vegetables (Etchells & Bell, 1950). Acromyrmex lundii is an ant species in the tribe Attini (Hymenoptera: Formicidae), which have a mutualistic relationship with basidiomycetous fungi in the order Agaricales (Bonetto, 1959).…”

Wickerhamomyces siamensis sp. nov., a novel yeast species isolated from the phylloplane in Thailand

“…The genus Wickerhamomyces was proposed to accommodate 17 species that were transferred from the genera Pichia , Williopsis and Hansenula as a result of phylogenetic analysis of nucleotide divergence in the genes coding for the large-subunit (LSU) and small-subunit rRNAs and for elongation factor 1α (Kurtzman et al , 2008; Kurtzman, 2011). The number of Wickerhamomyces species was subsequently increased by the discovery of Wickerhamomyces edaphicus (Limtong et al , 2009), W. queroliae (Rosa et al , 2009), W. patagonicus (de García et al , 2010), W. chaumierensis (Groenewald et al , 2011), W. ochangensis (Shin et al , 2011), W. tratensis (Nakase et al , 2012) and W. xylosica (Limtong et al , 2012).…”