Wide-field and high-resolution optical imaging for early detection of oral neoplasia

Pierce, Mark C.; Schwarz, Richard A.; Rosbach, Kelsey; Roblyer, Darren; Muldoon, Timothy P.; Williams, Michelle D.; El‐Naggar, Adel K.; Gillenwater, Ann M.; Richards‐Kortum, Rebecca

doi:10.1117/12.848347

Cited by 1 publication

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“…12,20 The combination of large FOV imaging for macroscopic surveillance with high resolution imaging or point measurement techniques has the potential to improve diagnosis and diagnostic yield by guiding the effective point sampling technique to the site with the most advanced state of disease within a lesion or the oral cavity. 11,[21][22][23] Wide-field autofluorescence imaging instruments are commercially available for clinical oral cancer detection; of interest in epithelial precancer progression are collagen crosslinks in the stroma and metabolic cofactors nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) in epithelial cells. Increased cellular metabolism in epithelial dysplasia results in an increase in NADH and FAD fluorescence signal; conversely, in inflammation, the epithelial cell fluorescence decreases slightly.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer

et al. 2013

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Abstract. Optical imaging techniques using a variety of contrast mechanisms are under evaluation for early detection of epithelial precancer; however, tradeoffs in field of view (FOV) and resolution may limit their application. Therefore, we present a multiscale multimodal optical imaging system combining macroscopic biochemical imaging of fluorescence lifetime imaging (FLIM) with subcellular morphologic imaging of reflectance confocal microscopy (RCM). The FLIM module images a 16 × 16 mm 2 tissue area with 62.5 μm lateral and 320 ps temporal resolution to guide cellular imaging of suspicious regions. Subsequently, coregistered RCM images are acquired at 7 Hz with 400 μm diameter FOV, <1 μm lateral and 3.5 μm axial resolution. FLIM-RCM imaging was performed on a tissue phantom, normal porcine buccal mucosa, and a hamster cheek pouch model of oral carcinogenesis. While FLIM is sensitive to biochemical and macroscopic architectural changes in tissue, RCM provides images of cell nuclear morphology, all key indicators of precancer progression.

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