Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles

Turko, Nir A.; Peled, Anna; Shaked, Natan T.

doi:10.1117/1.jbo.18.11.111414

Cited by 21 publications

(29 citation statements)

References 36 publications

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“…Nonetheless, the use of additional exogenous agents, such as microspheres and nanoparticles, can be advantageous in these applications [110][111][112]143,144]. Polarization-sensitive imaging [145,146] or Raman spectroscopy [147] also can be employed in holographic microspectroscopy as an additional modality to include birefringence imaging contrast in addition to optical dispersion.…”

Section: Discussion and Perspectivesmentioning

confidence: 98%

Biomedical applications of holographic microspectroscopy [Invited]

Jung

et al. 2014

Appl. Opt.

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The identification and quantification of specific molecules are crucial for studying the pathophysiology of cells, tissues, and organs as well as diagnosis and treatment of diseases. Recent advances in holographic microspectroscopy, based on quantitative phase imaging or optical coherence tomography techniques, show promise for label-free noninvasive optical detection and quantification of specific molecules in living cells and tissues (e.g., hemoglobin protein). To provide important insight into the potential employment of holographic spectroscopy techniques in biological research and for related practical applications, we review the principles of holographic microspectroscopy techniques and highlight recent studies.

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Section: Discussion and Perspectivesmentioning

confidence: 98%

Biomedical applications of holographic microspectroscopy [Invited]

Jung

et al. 2014

Appl. Opt.

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“…Since a sequence of interferograms is acquired, a dynamic OPD map is obtained. The PT signal can then be extracted at the temporal frequency of the excitation source 15,29 .…”

Section: Experimental Systemmentioning

confidence: 99%

“…Because of the correlation between the local temperature change in the solution surrounding such a nanoparticle and the phase of the light interacting with the solution, it is possible to image these nanoparticles and detect their locations. Hence, imaging the nanoparticles as heat sources can be accomplished using phase-sensitive techniques such as differential interference contrast microscopy 11,12 , phase-sensitive optical coherence microscopy 13,14 , and wide-field interferometric phase microscopy 15 . In these PT phase-imaging methods, the nanoparticles are stimulated at their peak plasmonic wavelength by time-modulated illumination.…”

Section: Introductionmentioning

confidence: 99%

Prediction of photothermal phase signatures from arbitrary plasmonic nanoparticles and experimental verification

Blum

Shaked

2015

Light Sci Appl

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We present a new approach for predicting spatial phase signals originating from photothermally excited metallic nanoparticles of arbitrary shapes and sizes. The heat emitted from such a nanoparticle affects the measured optical phase signal via changes in both the refractive index and thickness of the nanoparticle surroundings. Because these particles can be bio-functionalized to bind certain biological cell components, they can be used for biomedical imaging with molecular specificity, as new nanoscopy labels, and for photothermal therapy. Predicting the ideal nanoparticle parameters requires a model that computes the thermal and phase distributions around the particle, thereby enabling more efficient phase imaging of plasmonic nanoparticles and avoiding trial-and-error experiments while using unsuitable nanoparticles. The proposed nonlinear model is the first to enable the prediction of phase signatures from nanoparticles with arbitrary parameters. The model is based on a finite-volume method for geometry discretization and an implicit backward Euler method for solving the transient inhomogeneous heat equation, followed by calculation of the accumulative phase signal. To validate the model, we compared its results with experimental results obtained for gold nanorods of various concentrations, which we acquired using a custom-built wide-field interferometric phase microscopy system. INTRODUCTIONPlasmonic nanoparticles are used in a variety of scientific disciplines because of their unique interactions with electromagnetic fields. Plasmonic nanoparticles are used in photonics to sense or induce changes in certain environments, thereby acting as either nanosensors or nanosources for changes in chemical, thermal, or material properties 1 . In biomedical applications, plasmonic nanoparticles can be used as labels in cells and tissues and are imaged via various effects, including photothermal (PT) imaging, photoacoustic shock-wave imaging, scattering, and polarization imaging 225 . The electromagnetic energy absorbed by a metallic nanoparticle is rapidly transformed into heat through electron-phonon relaxation; thus, nanoparticles in solution act as nanosources of heat, which can be manipulated for imaging or for destructive purposes.Several numerical models of nanoparticles have been proposed to estimate the transfer of heat from a nanoparticle submerged in a liquid to its surroundings 5-8 . These models can yield information on the processes the nanoparticles undergo on time scales or at resolutions smaller than those at which they can be measured. In addition, these models can predict the thermal distribution in the nanoparticle surroundings; thereby making it possible to explain phenomena observed when heated particles interact with other materials, particularly with biological materials that are prone to thermal damage.

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“…Multiple optical techniques have been established to detect NPs on multiple substrates [2]. These include, among others, dark-field microscopy [3], which takes advantage of enhanced NP scattering at their plasmonic resonance wavelength, and photothermal microscopy, which visualizes refractive index changes in the sample media due to localized NP heating [4–9]. One limitation of these approaches is that cellular structures are typically not visible.…”

Section: Introductionmentioning

confidence: 99%

Fast wide-field photothermal and quantitative phase cell imaging with optical lock-in detection

Eldridge¹,

Meiri²,

Sheinfeld³

et al. 2014

Biomed. Opt. Express

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We present a fast, wide-field holography system for detecting photothermally excited gold nanospheres with combined quantitative phase imaging. An interferometric photothermal optical lock-in approach (POLI) is shown to improve SNR for detecting nanoparticles (NPs) on multiple substrates, including a monolayer of NPs on a silanized coverslip, and NPs bound to live cells. Furthermore, the set up allowed for co-registered quantitative phase imaging (QPI) to be acquired in an off-axis holographic set-up. An SNR of 103 was obtained for NP-tagging of epidermal growth factor receptor (EGFR) in live cells with a 3 second acquisition, while an SNR of 47 was seen for 20 ms acquisition. An analysis of improvements in SNR due to averaging multiple frames is presented, which suggest that residual photothermal signal can be a limiting factor. The combination of techniques allows for high resolution imaging of cell structure via QPI with the ability to identify receptor expression via POLI.

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Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles

Cited by 21 publications

References 36 publications

Biomedical applications of holographic microspectroscopy [Invited]

Biomedical applications of holographic microspectroscopy [Invited]

Prediction of photothermal phase signatures from arbitrary plasmonic nanoparticles and experimental verification

Fast wide-field photothermal and quantitative phase cell imaging with optical lock-in detection

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