Widely used gene editing strategies in cancer treatment a systematic review

Suleiman, Ahmed AbdulJabbar; Saedi, Walaa Yahya; Muhaidi, Mohammed J.

doi:10.1016/j.genrep.2020.100983

Cited by 6 publications

(4 citation statements)

References 103 publications

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“…Moreover, these programs are limited to the examination of only homologous genes (Yee and Yee 2016;H. C. Yang and Chen 2018;Suleiman, Saedi, and Muhaidi 2021;Zischewski, Fischer, and Bortesi 2017). High-throughput NGS or genomewide next-generation sequencing can contribute to reducing off-target effects by designing extremely target-specific sgRNAs using existing gene sequences.…”

Section: Limitations and Their Possible Solutions In Engineering Frui...mentioning

confidence: 99%

Development of Climate Smart Fruit Plants via CRISPR/Cas Genome Editing Systems: A Spatiotemporal Review

Shahzaib¹,

Brügmann²,

Shakeel³

et al. 2023

Preprint

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Fruit production is an important part of the gross domestic product for many countries around the world especially to those who have a strong focus on agriculture. However, long-term maintenance and yield stability of fruit production may be threatened by the ongoing climate change and its consequences like extended drought periods, heavy rain events, and floodings. Genome editing, with its progressive technological developments, offers opportunities to adapt relevant fruit plant species to new climatic conditions. Among modern genome editing techniques, CRISPR/Cas, in particular, has the potential to support breeding for those fruit plant species with extended breeding cycles, e.g., perennial fruits. In this review, we discuss CRISPR/Cas and other genome editing techniques in detail and how these techniques can be applied to support the breeding of fruit plant species for adaptation to changing climates. The chronological history of CRISPR/Cas9 systems, their associated computational tools, genomic data sources, transformation methods along with their delivery vehicles, quality improvement, environmental-stress resiliency, limitations, and future perspectives will also be discussed with respect to securing future global fruit production.

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Section: Limitations and Their Possible Solutions In Engineering Frui...mentioning

confidence: 99%

Development of Climate Smart Fruit Plants via CRISPR/Cas Genome Editing Systems: A Spatiotemporal Review

Shahzaib¹,

Brügmann²,

Shakeel³

et al. 2023

Preprint

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“…The off-target effects are still a major concern in complex eukaryotic organisms, most often in vivo for therapeutic applications ( 34 , 35 ). The targeting specificity depends upon the gRNA of Cas9 and PAM sequences, and off-target cleavage in the genome ( 36 ). Different online editing programs have been developed and successfully utilized to identify and predict off-target cleavages in silico .…”

Section: Pros and Cons Of Crispr/cas Technologiesmentioning

confidence: 99%

CRISPR/Cas: Advances, Limitations, and Applications for Precision Cancer Research

Yang

et al. 2021

Front. Med.

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Cancer is one of the most leading causes of mortalities worldwide. It is caused by the accumulation of genetic and epigenetic alterations in 2 types of genes: tumor suppressor genes (TSGs) and proto-oncogenes. In recent years, development of the clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized genome engineering for different cancer research ranging for research ranging from fundamental science to translational medicine and precise cancer treatment. The CRISPR/CRISPR associated proteins (CRISPR/Cas) are prokaryote-derived genome editing systems that have enabled researchers to detect, image, manipulate and annotate specific DNA and RNA sequences in various types of living cells. The CRISPR/Cas systems have significant contributions to discovery of proto-oncogenes and TSGs, tumor cell epigenome normalization, targeted delivery, identification of drug resistance mechanisms, development of high-throughput genetic screening, tumor models establishment, and cancer immunotherapy and gene therapy in clinics. Robust technical improvements in CRISPR/Cas systems have shown a considerable degree of efficacy, specificity, and flexibility to target the specific locus in the genome for the desired applications. Recent developments in CRISPRs technology offers a significant hope of medical cure against cancer and other deadly diseases. Despite significant improvements in this field, several technical challenges need to be addressed, such as off-target activity, insufficient indel or low homology-directed repair (HDR) efficiency, in vivo delivery of the Cas system components, and immune responses. This study aims to overview the recent technological advancements, preclinical and perspectives on clinical applications of CRISPR along with their advantages and limitations. Moreover, the potential applications of CRISPR/Cas in precise cancer tumor research, genetic, and other precise cancer treatments discussed.

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“…Genome-editing nucleases can be modified to recognize and break the genome at specific DNA sequences, resulting in DSBs, which are efficiently repaired by either NHEJ or HDR. 10 , 11 …”

Section: Gene Editing Toolsmentioning

confidence: 99%

“…From a clinical viewpoint, HDR is favorable for restoring mutations in genes or for integrating genes for therapeutic purposes. 10–13 …”

Section: Gene Editing Toolsmentioning

confidence: 99%

The Current Status of Gene Therapy for the Treatment of Cancer

Belete¹

2021

BTT

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Gene therapy is the administration of foreign genomic material into the host tissue to modify the expression of a gene product or to change the biological properties of cells for therapeutic use. Initially, the major objective of gene therapy was to manage genetic diseases, but now different disorders with several patterns of acquired and inherited disorders are targets of gene therapy. Over three decades, the advancement of Genome engineering technologies facilitated gene therapy for the prevention and management of intractable diseases. Researchers are advancing with cautious optimism that safe and effective treatment will give to patients with single-gene disorders and complex acquired disorders. To date, over 3000 genes associates with disease-causing mutations, and about 2600 gene therapy trials are undergoing for the management of various disorders. This review summarizes the principles of genome-editing approaches, such as zinc finger nucleases, transcription activator-like effector nucleases, meganucleases, and the CRISPR/Cas9 system with the underlying mechanisms. This review also explains the types of gene delivery systems as viral [adenoviral, adeno association, herpes simplex virus] and nonviral delivery systems (physical: DNA bombardment, electroporation) and (chemical: Cationic lipids, cationic polymers). Finally, this review summarizes gene therapy medicines approved to treat cancer in detail, including names, indications, vectors, and mode of gene therapy. Gene therapy becomes an alternative to an existing management for different diseases. Therefore, gene products with safe vectors and better biotechnologies play a significant role in the prophylaxis and management of various disorders in the future.

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Widely used gene editing strategies in cancer treatment a systematic review

Cited by 6 publications

References 103 publications

Development of Climate Smart Fruit Plants via CRISPR/Cas Genome Editing Systems: A Spatiotemporal Review

Development of Climate Smart Fruit Plants via CRISPR/Cas Genome Editing Systems: A Spatiotemporal Review

CRISPR/Cas: Advances, Limitations, and Applications for Precision Cancer Research

The Current Status of Gene Therapy for the Treatment of Cancer

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