Widespread occurrence of honey bee pathogens in solitary bees

Ravoet, Jorgen; Smet, Lina De; Meeus, Ivan; Smagghe, Guy; Wenseleers, Tom; Graaf, Dirk C. de

doi:10.1016/j.jip.2014.08.007

Cited by 180 publications

(160 citation statements)

References 20 publications

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“…It concerns a newly found bee virus and its discovery was thus far limited to only the USA and Europe (Runckel et al 2011;Ravoet et al 2013;Granberg et al 2013). It seems to be a polymorphic viral species that also occurs in solitary bees (Ravoet et al 2014). The finding of an ABPV with an unusual molecular signature of the capsid region is in line with the expectations, as different closely related bee viruses, including ABPV, KBV and IAPV, are now considered part of a complex within the family of Dicistroviridae (de Miranda et al 2010).…”

Section: Discussionmentioning

confidence: 56%

“…First, RNA (5 μL) was reverse transcribed using the RevertAid ™ First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA) with random hexamer primers according to the manufacturer's instructions. Subsequent PCR reactions were done in a mixture of 1.5 mM MgCl 2 , 0.2 mM dNTP, 1.25 U Hotstar Taq DNA polymerase (Qiagen, Frederick, MD, USA), 1 μL cDNA product and the appropriate primer set (2 μM of each primer; primer sequences can be found in Ravoet et al 2014). Samples positive for the ABPV complex were reanalysed with primers covering parts of the capsid gene specific for ABPV, IAPV and Kashmir bee virus (KBV), designed by Singh et al (2010).…”

Section: Reverse Transcriptase-pcr and Sequencingmentioning

confidence: 99%

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Discovery of Lake Sinai virus and an unusual strain of acute bee paralysis virus in West African apiaries

et al. 2015

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-Samples of bees and honey from 101 apiaries were collected in the West African Republic of Benin. Visual inspection revealed high Varroa destructor and Aethina tumida infestations and low Oplostomus fuligineus infestations. A pathogen screen based on bacterial/fungal cultures or microscopic examinations revealed the absence of American and European foulbrood, chalkbrood disease, acariosis and nosemosis. However, 13 bee samples (15.47 %) were found to be infected by viruses as determined by PCR-based techniques. Acute bee paralysis virus (ABPV; 8.33 %) and Black queen cell virus (4.76 %) were the most common. From the former we found a strain with an unusual molecular signature of the capsid region, which was taxonomically positioned between ABPV and Israeli acute paralysis virus. We also discovered Lake Sinai virus (LSV; 3.57 %) for the first time in Africa. Eight honey samples collected in regions where cotton was cultivated were subjected to multi-residue and quality control analyses.honey bees / diseases / parasites / viruses / pesticide residue

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Section: Discussionmentioning

confidence: 56%

Section: Reverse Transcriptase-pcr and Sequencingmentioning

confidence: 99%

Discovery of Lake Sinai virus and an unusual strain of acute bee paralysis virus in West African apiaries

et al. 2015

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“…2013; Ravoet et al . 2014), and for at least one emerging RNA virus, disease in managed honeybees and wild bumblebees is linked (Fürst et al . 2014).…”

Section: Introductionmentioning

confidence: 99%

A sting in the spit: widespread cross‐infection of multipleRNAviruses across wild and managed bees

McMahon

Fürst

Caspar

et al. 2015

Journal of Animal Ecology

256

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Summary Declining populations of bee pollinators are a cause of concern, with major repercussions for biodiversity loss and food security. RNA viruses associated with honeybees represent a potential threat to other insect pollinators, but the extent of this threat is poorly understood.This study aims to attain a detailed understanding of the current and ongoing risk of emerging infectious disease (EID) transmission between managed and wild pollinator species across a wide range of RNA viruses.Within a structured large‐scale national survey across 26 independent sites, we quantify the prevalence and pathogen loads of multiple RNA viruses in co‐occurring managed honeybee (Apis mellifera) and wild bumblebee (Bombus spp.) populations. We then construct models that compare virus prevalence between wild and managed pollinators.Multiple RNA viruses associated with honeybees are widespread in sympatric wild bumblebee populations. Virus prevalence in honeybees is a significant predictor of virus prevalence in bumblebees, but we remain cautious in speculating over the principle direction of pathogen transmission. We demonstrate species‐specific differences in prevalence, indicating significant variation in disease susceptibility or tolerance. Pathogen loads within individual bumblebees may be high and in the case of at least one RNA virus, prevalence is higher in wild bumblebees than in managed honeybee populations.Our findings indicate widespread transmission of RNA viruses between managed and wild bee pollinators, pointing to an interconnected network of potential disease pressures within and among pollinator species. In the context of the biodiversity crisis, our study emphasizes the importance of targeting a wide range of pathogens and defining host associations when considering potential drivers of population decline.

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“…DWV has a near global distribution and is the most widespread of the currently described viruses that infect honeybees, often affecting between 50% and 75% of all honeybee hives [25,26]. In addition, the virus can spill over to other bees [27,28], thereby posing an additional threat. DWV is named after the characteristic wing deformities that can arise when honeybees are infected in & 2017 The Author(s) Published by the Royal Society.…”

Section: Introductionmentioning

confidence: 99%

Covert deformed wing virus infections have long-term deleterious effects on honeybee foraging and survival

et al. 2017

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Several studies have suggested that covert stressors can contribute to bee colony declines. Here we provide a novel case study and show using radiofrequency identification tracking technology that covert deformed wing virus (DWV) infections in adult honeybee workers seriously impact longterm foraging and survival under natural foraging conditions. In particular, our experiments show that adult workers injected with low doses of DWV experienced increased mortality rates, that DWV caused workers to start foraging at a premature age, and that the virus reduced the workers' total activity span as foragers. Altogether, these results demonstrate that covert DWV infections have strongly deleterious effects on honeybee foraging and survival. These results are consistent with previous studies that suggested DWV to be an important contributor to the ongoing bee declines in Europe and the USA. Overall, our study underlines the strong impact that covert pathogen infections can have on individual and group-level performance in bees.

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Widespread occurrence of honey bee pathogens in solitary bees

Cited by 180 publications

References 20 publications

Discovery of Lake Sinai virus and an unusual strain of acute bee paralysis virus in West African apiaries

Discovery of Lake Sinai virus and an unusual strain of acute bee paralysis virus in West African apiaries

A sting in the spit: widespread cross‐infection of multipleRNAviruses across wild and managed bees

Covert deformed wing virus infections have long-term deleterious effects on honeybee foraging and survival

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