Widespread Occurrence of Low Levels of Alternariol in Apple and Tomato Products, as Determined by Comparative Immunochemical Assessment using Monoclonal and Polyclonal Antibodies

Ackermann, Yvonne; Curtui, Valeriu; Dietrich, Richard; Groß, Matthias; Latif, Hadri; Märtlbauer, Erwin; Usleber, Ewald

doi:10.1021/jf201516f

Cited by 74 publications

(68 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the contrary, natural occurrence of 1, 2 and 3 has been reported in decayed apples and apple products such as juices, sauces and concentrates, albeit at trace level (2) or in rather low concentration ranges (1 and 3, <10 µg/kg). 3,5,7 Regarding grape juice, our results are largely in agreement with the previous reports. Whereas several studies only sporadically reported trace levels of 1 and 2, Asam et al 7 detected low levels of 3 (≤7 µg/kg) in all four red grape juice samples analyzed due to the highly sensitive stable isotope dilution assay (SIDA) applied.…”

supporting

confidence: 93%

Validated UPLC-MS/MS Methods To Quantitate Free and Conjugated Alternaria Toxins in Commercially Available Tomato Products and Fruit and Vegetable Juices in Belgium

Walravens

Mikula

Rychlik

et al. 2016

J. Agric. Food Chem.

View full text Add to dashboard Cite

Ultra-performance liquid chromatography tandem mass spectrometry and Quick, Easy, Cheap, Effective, Rugged and Safe based analytical methodologies to quantitate both free (alternariol (1), alternariol monomethyl ether (2), tenuazonic acid (3), tentoxin (4), altenuene (5), altertoxin-I (6)) and conjugated (sulfates and glucosides of 1 and 2) Alternaria toxins in fruit and vegetable juices and tomato products were developed and validated. Acceptable limits of quantitation (0.7-5.7 µg/kg), repeatability (RSD r < 15.7%), reproducibility (RSD R < 17.9%) and apparent recovery (87.0-110.6%) were obtained for all analytes in all matrices investigated. 129 commercial foodstuffs were analyzed, and 3 was detected in 100% of tomato product samples (

show abstract

supporting

confidence: 93%

Validated UPLC-MS/MS Methods To Quantitate Free and Conjugated Alternaria Toxins in Commercially Available Tomato Products and Fruit and Vegetable Juices in Belgium

Walravens

Mikula

Rychlik

et al. 2016

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Notably, limited information was found about pyrenochaetic acid A (PyA), alternarienonic acid (AIA), and alloTeA and isoALT, recently reported in food samples for the first time [31,105] and metabolites such as AOH3S, AOH3G, AME3S, and AME3G [47,69]. In this way, AOH, AME, TeA, and TEN have been shown to occur in food samples frequently [82,97,108], while the occurrence of ALT, isoALT, ATX-I, and AAL toxins is of much lower incidence, mainly due to shortcomings in current analytical methodologies. Slight differences were observed based on the analyzed food matrices.…”

Section: Most Reported Mycotoxinsmentioning

confidence: 99%

AlternariaMycotoxins in Food and Feed: An Overview

Escrivá

Oueslati

Font

et al. 2017

Journal of Food Quality

146

127

View full text Add to dashboard Cite

Alternaria is one of the major mycotoxigenic fungal genera with more than 70 reported metabolites. Alternaria mycotoxins showed notably toxicity, such as mutagenicity, carcinogenicity, induction of DNA strand break, sphingolipid metabolism disruption, or inhibition of enzymes activity and photophosphorylation. This review reports on the toxicity, stability, metabolism, current analytical methods, and prevalence of Alternaria mycotoxins in food and feed through the most recent published research. Half of the publications were focused on fruits, vegetables, and derived products-mainly tomato and apples-while cereals and cereal byproducts represented 38%. The most studied compounds were alternariol, alternariol methyl ether, tentoxin, and tenuazonic acid, but altenuene, altertoxins (I, II, and III), and macrosporin have been gaining importance in recent years. Solid-liquid extraction (50%) with acetonitrile or ethyl acetate was the most common extraction methodology, followed by QuEChERS and dilutiondirect injection (both 14%). High-and ultraperformance liquid chromatography coupled with tandem mass spectrometry was the predominant determination technique (80%). The highest levels of alternariol and alternariol methyl ether were found in lentils, oilseeds, tomatoes, carrots, juices, wines, and cereals. Tenuazonic acid highest levels were detected in cereals followed by beer, while alternariol, alternariol methyl ether, tenuazonic acid, and tentoxin were found in legumes, nuts, and oilseeds.

show abstract

“…The two mycotoxins are common contaminants of food such as grain and grain-based products, fruits/fruit juice, and vegetable products (in particular, tomato products) (21)(22)(23). They have been reported to exhibit cytotoxic, fetotoxic, teratogenic, and possible mutagenic and estrogenic effects (24)(25)(26)(27) and may contribute to development of human esophageal cancer (28).…”

mentioning

confidence: 99%

SnPKS19 Encodes the Polyketide Synthase for Alternariol Mycotoxin Biosynthesis in the Wheat Pathogen Parastagonospora nodorum

Chooi

Muria-Gonzalez

Mead

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

b Alternariol (AOH) is an important mycotoxin from the Alternaria fungi. AOH was detected for the first time in the wheat pathogen Parastagonospora nodorum in a recent study. Here, we exploited reverse genetics to demonstrate that SNOG_15829 (SnPKS19), a close homolog of Penicillium aethiopicum norlichexanthone (NLX) synthase gene gsfA, is required for AOH production. We further validate that SnPKS19 is solely responsible for AOH production by heterologous expression in Aspergillus nidulans. The expression profile of SnPKS19 based on previous P. nodorum microarray data correlated with the presence of AOH in vitro and its absence in planta. Subsequent characterization of the ⌬SnPKS19 mutants showed that SnPKS19 and AOH are not involved in virulence and oxidative stress tolerance. Identification and characterization of the P. nodorum SnPKS19 cast light on a possible alternative AOH synthase gene in Alternaria alternata and allowed us to survey the distribution of AOH synthase genes in other fungal genomes. We further demonstrate that phylogenetic analysis could be used to differentiate between AOH synthases and the closely related NLX synthases. This study provides the basis for studying the genetic regulation of AOH production and for development of molecular diagnostic methods for detecting AOH-producing fungi in the future.

show abstract

Widespread Occurrence of Low Levels of Alternariol in Apple and Tomato Products, as Determined by Comparative Immunochemical Assessment using Monoclonal and Polyclonal Antibodies

Cited by 74 publications

References 29 publications

Validated UPLC-MS/MS Methods To Quantitate Free and Conjugated Alternaria Toxins in Commercially Available Tomato Products and Fruit and Vegetable Juices in Belgium

Validated UPLC-MS/MS Methods To Quantitate Free and Conjugated Alternaria Toxins in Commercially Available Tomato Products and Fruit and Vegetable Juices in Belgium

AlternariaMycotoxins in Food and Feed: An Overview

SnPKS19 Encodes the Polyketide Synthase for Alternariol Mycotoxin Biosynthesis in the Wheat Pathogen Parastagonospora nodorum

Contact Info

Product

Resources

About