Widespread quinolone resistance among methicillin-resistant Staphylococcus aureus isolates in a general hospital

Shalit, Itamar; Berger, Stephen A.; Gorea, Alfred; H, Frimerman

doi:10.1128/aac.33.4.593

Cited by 164 publications

(75 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since quinolone-resistance is developing comparably fast in this group of S. aureus and, as also evident from this study, quinolone-resistance seems to be already frequent among MRSA [20,21], the broader clinical use (e.g. for prophylactic regimes) of the newer quinolones should be in parallel with a constant monitoring of staphylococcal resistance patterns in order to avoid selection and spread of MRSA.…”

Section: Methodssupporting

confidence: 57%

Occurrence of quinolone resistance inStaphylococcus aureusfrom nosocomial infection

Witte

Grimm²

1992

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYAmong 63 Staphylococcus aureus isolates (one isolate per one patient) counted from infections (from August to November 1991) in hospital T., eight exhibited resistance to fluoroquinolones. Seven of these quinolone-resistant isolates were multiply-and methicillin-resistant S. aureus (QR-MRSA). The results of phage-, plasmid-and genotyping (pulsed field electrophoresis) revealed that six different strain-clones of these MRSA were spread in the hospital. In vitro spontaneous mutants resistant to fluoroquinolones are 10-100-fold more frequent in MRSA than in other S. aureus when selected on isosensitest-agar containing 1 #g/ml of ciprofloxacin. However, the same mutant frequencies were found in strain 8325-4 with and without the mecA-determinant. The resistance phenotype was stable over 30 generations of subculture in nutrient broth as well in natural quinolone resistant MRSA as in mutants of other types of S. aureus selected in vitro. The phenotypic association of quinolone resistance and MRSA is rather likely due to a higher frequency of spontaneous resistant mutants which are present in natural populations of MRSA. Data of chemotherapy prior to the isolation of S. aureus show that three of seven patients from whom QR-MRSA were isolated were treated with a quinolone. In eight cases of infections with non-MRSA and quinolone treatment the isolated S. aureus strains were in vitro sensitive to quinolones.

show abstract

Section: Methodssupporting

confidence: 57%

Occurrence of quinolone resistance inStaphylococcus aureusfrom nosocomial infection

Witte

Grimm²

1992

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…The prevalence of strains of S. aureus resistant to conventional antibiotics has increased to high levels in some hospitals [20,21]. In nosocomial, this bacterium is one of the most prevalent microorganism's worldwide [22].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activities of the Henna Extract and Some Synthetic Naphthoquinones Derivatives

Rahmoun¹,

Boucherit-Atmani²,

Benabdallah³

et al. 2013

AJMBR

View full text Add to dashboard Cite

Naphthoquinones are compounds widely distributed in the environment, both as natural products and as pharmaceutical agents. They have been the subject of much research due to their pharmacological activities. In this study, methanol extract of henna and a series of synthesized structural analogue of lawsone have been assessed for their antimicrobial activities. Methanol extract of henna and eight naphthoquinones derivatives were tested as potential antimicrobial agents against twelve bacteria and three Candida species using the agar disc diffusion and broth microdilution methods according to guidelines recommended by the Clinical and Laboratory Standards Institute. Besides 2b and 3b, methanol extract and all the synthesized compounds 1a, 2a, 1b and 1c, 2c and 3c showed weakto-strong activity against at least one tested strain. However, the compounds 1c and 1b were found to have the most effective activity against pathogenic bacteria and displayed an activity 8 to 64 fold higher than that othe structural analogue, lawsone. Their MICs ranged from 8-64 µg ml -1. Henna extract was found to have an interesting activity. Our results indicate an effective in vitro activity of 1,4-naphthoquinone derivatives and suggest the benefits of further studies for its application in antibiotherapy.

show abstract

“…However, similar to experience with earlier quinolones such as nalidixic acid, the isolation of S. aureus strains resistant to fluoroquinolones is now commonplace (8,11,12 Med., in press). The emergence of fluoroquinolone resistance is of particular concern given that relatively few antimicrobial agents are effective against methicillin-resistant staphylococcal infections.…”

mentioning

confidence: 97%

DNA gyrase gyrA mutations in ciprofloxacin-resistant strains of Staphylococcus aureus: close similarity with quinolone resistance mutations in Escherichia coli

et al. 1990

View full text Add to dashboard Cite

The gyrA genes isolated from three ciprofloxacin-resistant clinical isolates of Staphylococcus aureus carried codon 84 (serine-leucine) and/or codon 85 (serine-*proline) mutations that were absent in pretreatment susceptible strains. These substitutions occur in a region of the gyrase A protein wherein directly analogous mutations of serine 83-*leucine and alanine 84->proline in Escherichia coli confer quinolone resistance. Thus, DNA gyrase A subunit mutations are implicated in resistance to ciprofloxacin in S. aureus.

show abstract

Widespread quinolone resistance among methicillin-resistant Staphylococcus aureus isolates in a general hospital

Abstract: Ofloxacin and ciprofloxacin resistance (MIC,>4 ,ug/ml)

Cited by 164 publications

References 11 publications

Occurrence of quinolone resistance inStaphylococcus aureusfrom nosocomial infection

Occurrence of quinolone resistance inStaphylococcus aureusfrom nosocomial infection

Antimicrobial Activities of the Henna Extract and Some Synthetic Naphthoquinones Derivatives

DNA gyrase gyrA mutations in ciprofloxacin-resistant strains of Staphylococcus aureus: close similarity with quinolone resistance mutations in Escherichia coli

Contact Info

Product

Resources

About