Width as an alternative measurement to length for post-mortem interval estimations using Calliphora augur (Diptera: Calliphoridae) larvae

Day, Donnah M.; Wallman, James F.

doi:10.1016/j.forsciint.2005.07.009

Cited by 52 publications

(28 citation statements)

References 47 publications

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“…Because the largest immature blowfly is an outlier, it is not the most representative measure of the duration of development and should not be used to calculate thermal summation constants. It is imperative that authors either sample randomly (all sizes of a single cohort) [15] or sample all individuals [19] during experimental data collection to better understand the variation in size with respect to actual age.…”

Section: Discussionmentioning

confidence: 99%

Factors affecting accuracy and precision of thermal summation models of insect development used to estimate post-mortem intervals

Richards

Villet

2008

Int J Legal Med

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This paper investigates the effects that different summary statistics (minimum, median, mean, or maximum), temporal sampling resolutions (duration between sampling events), and sample sizes (number of individuals sampled per sampling event) had on the accuracy and precision of the regression coefficients of a typical thermal summation model used to calculate minimum post-mortem interval (PMI). No significant differences were found in the values of the developmental constants calculated from different summary statistics of the duration of development. Sample size was found to affect the precision of measurement of the duration of development but had little overall influence on thermal summation constant (K) and developmental threshold (D0) calculations (and therefore, subsequent PMI estimates), but temporal sampling resolution had a direct influence on the accuracy of K and D0 calculations. These data suggest that when numbers of experimental maggots are limited, it is more important to sample more frequently using smaller sample sizes than to sample less frequently with large sample sizes. Furthermore, we suggest that the median is the most representative summary measure of the duration of development and should be used preferentially.

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Section: Discussionmentioning

confidence: 99%

Factors affecting accuracy and precision of thermal summation models of insect development used to estimate post-mortem intervals

Richards

Villet

2008

Int J Legal Med

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“…Collected larvae were immediately killed by immersion in boiling water, dried with paper towel and preserved in 80% EtOH [18,19]. Larvae were placed into vials posteriad to prevent head curling [20]. Where larval growth stages differed behaviourally (i.e.…”

Section: Introductionmentioning

confidence: 99%

A comparison of frozen/thawed and fresh food substrates in development of Calliphora augur (Diptera: Calliphoridae) larvae

Day

Wallman

2006

Int J Legal Med

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While research has examined the effect of freezing and subsequent thawing on the decomposition of carcasses, no studies have investigated the effect of the freezing and thawing of tissues used as a developmental substrate by fly larvae. This paper reports on the results of such a study using larvae of Calliphora augur (Fabricius) on sheep's liver. Approximately 20 first-instar larvae were collected on sheep liver and subsequently transferred to paired treatments of fresh and frozen/thawed liver equilibrated to room temperature. They were then left undisturbed for 1-10 days. When the allocated time had elapsed, the body length of the larvae in each pair of groups was compared. No significant differences were detected between any pairs at a 1% level and only one pair was significantly different at a 5% level. We conclude that freezing and thawing of a developmental medium of sheep's liver has no significant effect on growth of C. augur larvae.

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“…The reason that the first-instars were not also significantly different is unknown but could relate to a metabolic delay between being deposited and assimilation of feeding products into epidermal and cuticle elements. We have observed first-instars to have a reduced width shortly after larviposition [53], which appears to be related to such a time lag.…”

Section: Different Growth Stages Of C Augur Preserved In 80% Etohmentioning

confidence: 70%

“…Larvae should then be removed from the initial alcohol solution and placed into boiling water for about 30 s. After this time, they should be promptly removed and placed into a fresh solution of 80% alcohol, in which they will be properly fixed and ready for long-term storage or shipment [31]. Immersion and swirling of larvae in hot water may also lessen the occurrence of head-curling [53]. In an emergency, a cup of hot water or tea can be obtained from fast food outlets (M.L.…”

Section: 2mentioning

confidence: 99%

Effect of preservative solutions on preservation of Calliphora augur and Lucilia cuprina larvae (Diptera: Calliphoridae) with implications for post-mortem interval estimates

Day

Wallman

2008

Forensic Science International

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Width as an alternative measurement to length for post-mortem interval estimations using Calliphora augur (Diptera: Calliphoridae) larvae

Cited by 52 publications

References 47 publications

Factors affecting accuracy and precision of thermal summation models of insect development used to estimate post-mortem intervals

Factors affecting accuracy and precision of thermal summation models of insect development used to estimate post-mortem intervals

A comparison of frozen/thawed and fresh food substrates in development of Calliphora augur (Diptera: Calliphoridae) larvae

Effect of preservative solutions on preservation of Calliphora augur and Lucilia cuprina larvae (Diptera: Calliphoridae) with implications for post-mortem interval estimates

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