Wildlife Carnivorous Mammals As a Specific Mirror of Environmental Contamination with Multidrug-Resistant <i>Escherichia coli</i> Strains in Poland

Osińska, Marcelina; Nowakiewicz, Aneta; Zięba, Przemysław; Gnat, Sebastian; Łagowski, Dominik; Trościańczyk, Aleksandra

doi:10.1089/mdr.2019.0480

Cited by 10 publications

(15 citation statements)

References 45 publications

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“…This is a lower percentage than that in a group of wildlife predators analysed previously, in which we observed resistance to at least one drug in approx. 70% of the tested animals (Osińska et al, 2020). However, the lower number of resistant isolates in herbivores and omnivores compared to carnivores is consistent with studies in mammals (Nhung et al, 2015) and birds (Smith, Wang, Fanning, & McMahon, 2014).…”

Section: Discussionsupporting

confidence: 86%

“…Isolation and identification of resistant E. coli strains were carried out according to a procedure described earlier (Osińska et al, 2020). The material was inoculated on selective MacConkey agar media (Biomaxima, Lublin, Poland) supplemented with tetracycline (8 mg/L), chloramphenicol (16 mg/L), kanamycin (32 mg/L), and cefotaxime (2 mg/L) (Sigma Aldrich, Germany).…”

Section: Isolation Identification and Selection Of Strainsmentioning

confidence: 99%

“…The material was inoculated on selective MacConkey agar media (Biomaxima, Lublin, Poland) supplemented with tetracycline (8 mg/L), chloramphenicol (16 mg/L), kanamycin (32 mg/L), and cefotaxime (2 mg/L) (Sigma Aldrich, Germany). Single isolates were selected as described in previous studies (Nowakiewicz et al, 2020;Osińska et al, 2020) using a two-step strain selection approach: the disc diffusion method (DDM) and Amplification of DNA Fragments Surrounding Rare Restriction Sites (ADSRRS).…”

Section: Isolation Identification and Selection Of Strainsmentioning

confidence: 99%

“…In the second step, based on a similarity value below 90%, the ADSRRS-fingerprinting method was used for distinguishing separate genotypes among strains isolated from the same individual as described in a previous study (Osińska et al, 2020).…”

Section: Isolation Identification and Selection Of Strainsmentioning

confidence: 99%

“…For example, according to the ECDC report, the number of E. coli strains resistant to the extended spectrum of β-lactam antibiotics (ESBL) and fluoroquinolones exhibits a growing trend in Poland as well (ECDC, 2019). Moreover, there are many reports of carriage and transmission of multidrug-resistant E. coli strains between different host species both in our country and in the world (Nowakiewicz et al, 2020;Hassell et al, 2019;Osińska et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Do wildlife omnivores and herbivores serve as a source of multidrug-resistant and pathogenic Escherichia coli strains in Poland? Analysis with reference to the host species range

Osińska

Nowakiewicz

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et al. 2020

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The phenomenon of resistance of Escherichia coli strains in free-living animals has been constantly expanding in recent years. However, the data is still fragmented and, due to the growing threat to public health, there is a constant need to search for and analyse new reservoirs and indicate their role and importance in the circulation of resistance genes. Therefore, the target group in this study were free-living non-predatory animals as reservoirs of drug-resistant and potentially virulent E. coli strains. For isolation, we used a combination of selective media with four antimicrobials. After additional two-stage analysis of strain similarity, we obtained 70 different isolates, including 71.4% of multidrug-resistant strains. In strains isolated from all species of animals, we determined high resistance to ampicillin (95.7%), tetracycline (64.3%), streptomycin (51.4%), and chloramphenicol (38.6%). Every third of the E. coli-positive individual was a carrier of more than one resistant clone. Moreover, four isolates among the resistant strains had the ExPEC pathotype, two had the ETEC pathotype, and another two represented EHEC.Our study confirmed that not only free-living predatory animals are reservoirs of resistance but also many synanthropic species of herbivores and omnivores contribute substantially to the spread of resistant and virulent E. coli strains.

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Section: Discussionsupporting

confidence: 86%

Section: Isolation Identification and Selection Of Strainsmentioning

confidence: 99%

Section: Isolation Identification and Selection Of Strainsmentioning

confidence: 99%

Section: Isolation Identification and Selection Of Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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