Through field perception in winter and summer, people’s preferences for vegetation types in green spaces with various structures (single-layer woodland, tree-shrub-grass composite woodland, tree-grass composite woodland, and single-layer grassland) and concrete and asphalt sites (areas) without plants are evaluated. The results show the following: (1) There are significant differences in landscape preference between the two seasons, and the environmental perception preference score for landscapes in summer is higher. (2) The preference score for the vegetation types in green spaces was single-layer woodland > tree-shrub-grass composite woodland > tree-grass composite woodland > single-layer grassland > concrete and asphalt sites (areas). The preference score for the single-layer woodland landscape in winter was significantly higher than that in summer. (3) The preferences of participants to carry out activities vary by season and the magnitude of thermal comfort; notably, rest and communication activities in green spaces with high thermal comfort, such as tree-shrub-grass composite woodland and single-layer woodland areas, are preferred in summer, and physical activities in the single-layer woodland landscape are preferred in winter. (4) Regardless of which environmental perception method is adopted, landscape preferences did not significantly differ by sex or professional background. Based on the above results, the two seasonal perception methods cannot replace each other. Therefore, the types of activities should be carefully selected to be appropriate for the specific vegetation types in green spaces and season in which they will occur, and the green space should be reasonably planned according to the thermal comfort of the vegetation types to provide a scientific basis for evaluating landscape perception and preferences in the future.